UniProtKB - Q2HJF5 (DPH6_BOVIN)
Protein
Diphthine--ammonia ligase
Gene
DPH6
Organism
Bos taurus (Bovine)
Status
Functioni
Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (EEF2) to diphthamide (By similarity).By similarity
Catalytic activityi
- ATP + diphthine-[translation elongation factor 2] + NH4+ = AMP + diphosphate + diphthamide-[translation elongation factor 2] + H+EC:6.3.1.14
: peptidyl-diphthamide biosynthesis Pathwayi
This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diphthine-ammonia ligase activity Source: GO_Central
GO - Biological processi
- peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-BTA-5358493, Synthesis of diphthamide-EEF2 |
UniPathwayi | UPA00559 |
Names & Taxonomyi
Protein namesi | Recommended name: Diphthine--ammonia ligase (EC:6.3.1.14)Alternative name(s): ATP-binding domain-containing protein 4 Diphthamide synthase Diphthamide synthetase Protein DPH6 homolog |
Gene namesi | Name:DPH6 Synonyms:ATPBD4 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:28181, DPH6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282396 | 1 – 267 | Diphthine--ammonia ligaseAdd BLAST | 267 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 97 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q2HJF5 |
PRIDEi | Q2HJF5 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000001364, Expressed in longissimus thoracis muscle and 19 other tissues |
Family & Domainsi
Sequence similaritiesi
Belongs to the Diphthine--ammonia ligase family.Curated
Phylogenomic databases
eggNOGi | KOG2316, Eukaryota |
GeneTreei | ENSGT00420000029820 |
HOGENOMi | CLU_010289_0_1_1 |
InParanoidi | Q2HJF5 |
OMAi | LDESWLG |
OrthoDBi | 1126213at2759 |
TreeFami | TF313566 |
Family and domain databases
CDDi | cd01994, Alpha_ANH_like_IV, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002761, Diphthami_syn_dom IPR030662, DPH6/MJ0570 IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR12196, PTHR12196, 1 hit |
Pfami | View protein in Pfam PF01902, Diphthami_syn_2, 1 hit |
PIRSFi | PIRSF039123, Diphthamide_synthase, 1 hit |
TIGRFAMsi | TIGR00290, MJ0570_dom, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2HJF5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVAALISGG KDSCYNMMQC VAAGHQIVAL ANLRPAENQV GSDELDSYMY
60 70 80 90 100
QTVGHHAIDL YAEAMALPLY RRTIRGKSVD TGPVYTKCEG DEVEDLYELL
110 120 130 140 150
KLVKEKEEVE GISVGAILSD YQRVRVENVC KRLNLQPLAY LWQRNQEDLL
160 170 180 190 200
QEMISSNIQA IIIKVAALGL DPDKHLGKPL DQMEPYLLEL SKKYGVHVCG
210 220 230 240 250
EGGEYETFTL DCPLFKKKII VDSSEVVTHS ADAFAPVAYL RFLELHLEDK
260
VSPVPDNCRT SNDIHNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC105462 mRNA Translation: AAI05463.1 |
RefSeqi | NP_001070484.1, NM_001077016.1 |
Genome annotation databases
Ensembli | ENSBTAT00000055382; ENSBTAP00000051676; ENSBTAG00000001364 |
GeneIDi | 767944 |
KEGGi | bta:767944 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC105462 mRNA Translation: AAI05463.1 |
RefSeqi | NP_001070484.1, NM_001077016.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000051676 |
Proteomic databases
PaxDbi | Q2HJF5 |
PRIDEi | Q2HJF5 |
Genome annotation databases
Ensembli | ENSBTAT00000055382; ENSBTAP00000051676; ENSBTAG00000001364 |
GeneIDi | 767944 |
KEGGi | bta:767944 |
Organism-specific databases
CTDi | 89978 |
VGNCi | VGNC:28181, DPH6 |
Phylogenomic databases
eggNOGi | KOG2316, Eukaryota |
GeneTreei | ENSGT00420000029820 |
HOGENOMi | CLU_010289_0_1_1 |
InParanoidi | Q2HJF5 |
OMAi | LDESWLG |
OrthoDBi | 1126213at2759 |
TreeFami | TF313566 |
Enzyme and pathway databases
UniPathwayi | UPA00559 |
Reactomei | R-BTA-5358493, Synthesis of diphthamide-EEF2 |
Gene expression databases
Bgeei | ENSBTAG00000001364, Expressed in longissimus thoracis muscle and 19 other tissues |
Family and domain databases
CDDi | cd01994, Alpha_ANH_like_IV, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002761, Diphthami_syn_dom IPR030662, DPH6/MJ0570 IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR12196, PTHR12196, 1 hit |
Pfami | View protein in Pfam PF01902, Diphthami_syn_2, 1 hit |
PIRSFi | PIRSF039123, Diphthamide_synthase, 1 hit |
TIGRFAMsi | TIGR00290, MJ0570_dom, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPH6_BOVIN | |
Accessioni | Q2HJF5Primary (citable) accession number: Q2HJF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | March 21, 2006 | |
Last modified: | April 7, 2021 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families