UniProtKB - Q2G0Q0 (PDXT_STAA8)
Protein
Pyridoxal 5'-phosphate synthase subunit PdxT
Gene
pdxT
Organism
Staphylococcus aureus (strain NCTC 8325 / PS 47)
Status
Functioni
Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation
Catalytic activityi
- aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H+ + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphateUniRule annotationEC:4.3.3.6UniRule annotation
- EC:3.5.1.2UniRule annotation
: pyridoxal 5'-phosphate biosynthesis Pathwayi
This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 75 | NucleophileUniRule annotation | 1 | |
Binding sitei | 101 | L-glutamineUniRule annotation | 1 | |
Active sitei | 165 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 167 | Charge relay systemUniRule annotation | 1 |
GO - Molecular functioni
- glutaminase activity Source: UniProtKB-UniRule
- pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity Source: UniProtKB-UniRule
GO - Biological processi
- glutamine catabolic process Source: UniProtKB-UniRule
- pyridoxal phosphate biosynthetic process Source: GO_Central
- pyridoxine metabolic process Source: GO_Central
- vitamin B6 biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Lyase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
UniPathwayi | UPA00245 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdxTUniRule annotation Ordered Locus Names:SAOUHSC_00500 |
Organismi | Staphylococcus aureus (strain NCTC 8325 / PS 47) |
Taxonomic identifieri | 93061 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- glutaminase complex Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000255838 | 1 – 186 | Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST | 186 |
Interactioni
Subunit structurei
In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 1280.SAXN108_0573 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 46 – 48 | L-glutamine bindingUniRule annotation | 3 | |
Regioni | 129 – 130 | L-glutamine bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the glutaminase PdxT/SNO family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | COG0311, Bacteria |
HOGENOMi | CLU_069674_2_0_9 |
OMAi | PVYGTCA |
Family and domain databases
CDDi | cd01749, GATase1_PB, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01615, PdxT, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR002161, PdxT/SNO IPR021196, PdxT/SNO_CS |
PANTHERi | PTHR31559, PTHR31559, 1 hit |
Pfami | View protein in Pfam PF01174, SNO, 1 hit |
PIRSFi | PIRSF005639, Glut_amidoT_SNO, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR03800, PLP_synth_Pdx2, 1 hit |
PROSITEi | View protein in PROSITE PS01236, PDXT_SNO_1, 1 hit PS51130, PDXT_SNO_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2G0Q0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIGVLALQG AVREHIRHIE LSGHEGIAVK KVEQLEEIEG LILPGGESTT
60 70 80 90 100
LRRLMNLYGF KEALQNSTLP MFGTCAGLIV LAQDIVGEEG YLNKLNITVQ
110 120 130 140 150
RNSFGRQVDS FETELDIKGI ATDIEGVFIR APHIEKVGQG VDILCKVNEK
160 170 180
IVAVQQGKYL GVSFHPELTD DYRVTDYFIN HIVKKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000253 Genomic DNA Translation: ABD29649.1 |
RefSeqi | WP_000690439.1, NZ_LS483365.1 YP_499073.1, NC_007795.1 |
Genome annotation databases
EnsemblBacteriai | ABD29649; ABD29649; SAOUHSC_00500 |
GeneIDi | 3920412 45573791 |
KEGGi | sao:SAOUHSC_00500 |
PATRICi | fig|93061.5.peg.447 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000253 Genomic DNA Translation: ABD29649.1 |
RefSeqi | WP_000690439.1, NZ_LS483365.1 YP_499073.1, NC_007795.1 |
3D structure databases
SMRi | Q2G0Q0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1280.SAXN108_0573 |
Genome annotation databases
EnsemblBacteriai | ABD29649; ABD29649; SAOUHSC_00500 |
GeneIDi | 3920412 45573791 |
KEGGi | sao:SAOUHSC_00500 |
PATRICi | fig|93061.5.peg.447 |
Phylogenomic databases
eggNOGi | COG0311, Bacteria |
HOGENOMi | CLU_069674_2_0_9 |
OMAi | PVYGTCA |
Enzyme and pathway databases
UniPathwayi | UPA00245 |
Miscellaneous databases
PROi | PR:Q2G0Q0 |
Family and domain databases
CDDi | cd01749, GATase1_PB, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01615, PdxT, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR002161, PdxT/SNO IPR021196, PdxT/SNO_CS |
PANTHERi | PTHR31559, PTHR31559, 1 hit |
Pfami | View protein in Pfam PF01174, SNO, 1 hit |
PIRSFi | PIRSF005639, Glut_amidoT_SNO, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR03800, PLP_synth_Pdx2, 1 hit |
PROSITEi | View protein in PROSITE PS01236, PDXT_SNO_1, 1 hit PS51130, PDXT_SNO_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDXT_STAA8 | |
Accessioni | Q2G0Q0Primary (citable) accession number: Q2G0Q0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | March 21, 2006 | |
Last modified: | December 2, 2020 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families