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UniProtKB - Q2FZW6 (DLTA_STAA8)
Protein
D-alanine--D-alanyl carrier protein ligase
Gene
dltA
Organism
Staphylococcus aureus (strain NCTC 8325 / PS 47)
Status
Functioni
Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
UniRule annotationCatalytic activityi
- ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + D-alanyl-[D-alanyl-carrier protein] + diphosphateUniRule annotationEC:6.2.1.54UniRule annotation
: lipoteichoic acid biosynthesis Pathwayi
This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 189 | D-alanineUniRule annotation | 1 | |
Binding sitei | 293 | D-alanine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 365 | ATPUniRule annotation | 1 | |
Binding sitei | 473 | ATPUniRule annotation | 1 | |
Binding sitei | 473 | D-alanineUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 144 – 145 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 284 – 289 | ATPUniRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- D-alanine [D-alanyl carrier protein] ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- lipoteichoic acid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00556 |
Names & Taxonomyi
Protein namesi | Recommended name: D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.54UniRule annotation)Short name: DCLUniRule annotation Alternative name(s): D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation D-alanine-activating enzymeUniRule annotation Short name: DAEUniRule annotation |
Gene namesi | Name:dltAUniRule annotation Ordered Locus Names:SAOUHSC_00869 |
Organismi | Staphylococcus aureus (strain NCTC 8325 / PS 47) |
Taxonomic identifieri | 93061 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289546 | 1 – 485 | D-alanine--D-alanyl carrier protein ligaseAdd BLAST | 485 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1020, Bacteria |
HOGENOMi | CLU_000022_2_12_9 |
OMAi | VMDLYPC |
Family and domain databases
CDDi | cd05945, DltA, 1 hit |
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
HAMAPi | MF_00593, DltA, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR010072, DltA IPR044507, DltA-like |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit TIGR01734, D-ala-DACP-lig, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2FZW6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP
60 70 80 90 100
MILFGHMSPY MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF
110 120 130 140 150
NTTDESFESL EGEVFTIEDI KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE
160 170 180 190 200
PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW LNQAPFSFDL SVMAIYPCLA
210 220 230 240 250
SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM CLLLPTLNEE
260 270 280 290 300
QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI
310 320 330 340 350
TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE
360 370 380 390 400
VFNFDDGIRT YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL
410 420 430 440 450
RQSEFVKEAI VVPVYKNDKV IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR
460 470 480
LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF101234 Genomic DNA Translation: AAD21957.1 CP000253 Genomic DNA Translation: ABD29994.1 |
RefSeqi | WP_000129653.1, NZ_LS483365.1 YP_499422.1, NC_007795.1 |
Genome annotation databases
EnsemblBacteriai | ABD29994; ABD29994; SAOUHSC_00869 |
GeneIDi | 3919216 |
KEGGi | sao:SAOUHSC_00869 |
PATRICi | fig|93061.5.peg.789 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF101234 Genomic DNA Translation: AAD21957.1 CP000253 Genomic DNA Translation: ABD29994.1 |
RefSeqi | WP_000129653.1, NZ_LS483365.1 YP_499422.1, NC_007795.1 |
3D structure databases
AlphaFoldDBi | Q2FZW6 |
SMRi | Q2FZW6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1280.SAXN108_0927 |
Genome annotation databases
EnsemblBacteriai | ABD29994; ABD29994; SAOUHSC_00869 |
GeneIDi | 3919216 |
KEGGi | sao:SAOUHSC_00869 |
PATRICi | fig|93061.5.peg.789 |
Phylogenomic databases
eggNOGi | COG1020, Bacteria |
HOGENOMi | CLU_000022_2_12_9 |
OMAi | VMDLYPC |
Enzyme and pathway databases
UniPathwayi | UPA00556 |
Miscellaneous databases
PROi | PR:Q2FZW6 |
Family and domain databases
CDDi | cd05945, DltA, 1 hit |
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
HAMAPi | MF_00593, DltA, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR010072, DltA IPR044507, DltA-like |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit TIGR01734, D-ala-DACP-lig, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DLTA_STAA8 | |
Accessioni | Q2FZW6Primary (citable) accession number: Q2FZW6 Secondary accession number(s): P68877, Q53661, Q9S673 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | March 21, 2006 | |
Last modified: | May 25, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families