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Entry version 94 (02 Jun 2021)
Sequence version 1 (21 Mar 2006)
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Protein

DNA topoisomerase 3

Gene

topB

Organism
Staphylococcus aureus (strain NCTC 8325 / PS 47)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation EC:5.6.2.1

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi8Magnesium; catalyticUniRule annotation1
Metal bindingi104Magnesium; catalyticUniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei305O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.6.2.1UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:SAOUHSC_02517
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStaphylococcus aureus (strain NCTC 8325 / PS 47)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri93061 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000008816 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002863681 – 711DNA topoisomerase 3Add BLAST711

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei60Interaction with DNAUniRule annotation1
Sitei167Interaction with DNAUniRule annotation1
Sitei175Interaction with DNAUniRule annotation1
Sitei307Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
1280.SAXN108_2503

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q2FW03

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 135ToprimUniRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni186 – 191Interaction with DNAUniRule annotation6
Regioni691 – 711DisorderedSequence analysisAdd BLAST21

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0550, Bacteria
COG0551, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_002929_5_2_9

Identification of Orthologs from Complete Genome Data

More...
OMAi
TYPRVDT

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00186, TOP1Ac, 1 hit
cd03362, TOPRIM_TopoIA_TopoIII, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00953, Topoisom_3_prok, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR005738, TopoIII
IPR006171, TOPRIM_domain
IPR034144, TOPRIM_TopoIII

The PANTHER Classification System

More...
PANTHERi
PTHR11390, PTHR11390, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00417, PRTPISMRASEI

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56712, SSF56712, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01056, topB, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q2FW03-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSLILAEKP SVARDIADAL QINQKRNGYF ENNQYIVTWA LGHLVTNATP
60 70 80 90 100
EQYDKNLKEW RLEDLPIIPK YMKTVVIGKT SKQFKTVKAL ILDNKVKDII
110 120 130 140 150
IATDAGREGE LVARLILDKV GNKKPIRRLW ISSVTKKAIQ QGFKNLKDGR
160 170 180 190 200
QYNDLYYAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVNT
210 220 230 240 250
RQQEINQFKP QQYFTLSLTV KGFDFQLESN QRYTNKETLE QMVNNLKNVD
260 270 280 290 300
GKIKSVATKH KKSYPQSLYN LTDLQQDMYR RYKIGPKETL NTLQSLYERH
310 320 330 340 350
KVVTYPRTDS NYLTTDMVDT MKERIQVTMA TTYKDQARPL MSKTFSSKMS
360 370 380 390 400
IFNNQKVSDH HAIIPTEVRP VMSDLSNREL KLYDMIVERF LEALMPPHEY
410 420 430 440 450
DAITVTLEVA GHTFVLKENV TTVLGFKSIR QGESITEMQQ PFSEGDEVKI
460 470 480 490 500
SKTNIREHET TPPEYFNEGS LLKAMENPQN FIQLKDKKYA QTLKQTGGIG
510 520 530 540 550
TVATRADIID KLFNMNAIES RDGKIKVTSK GKQILELAPE ELTSPLLTAQ
560 570 580 590 600
WEEKLLLIER GKYQAKTFIN EMKDFTKDVV NGIKNSDRKY KHDNLTTTEC
610 620 630 640 650
PTCGKFMIKV KTKNGQMLVC QDPSCKTKKN VQRKTNARCP NCKKKLTLFG
660 670 680 690 700
KGKEAVYRCV CGHSETQAHM DQRMKSKSSG KVSRKEMKKY MNKNEGLDNN
710
PFKDALKNLN L
Length:711
Mass (Da):81,552
Last modified:March 21, 2006 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE83A8F21CFE6918A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CP000253 Genomic DNA Translation: ABD31533.1

NCBI Reference Sequences

More...
RefSeqi
WP_000838485.1, NZ_LS483365.1
YP_500982.1, NC_007795.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ABD31533; ABD31533; SAOUHSC_02517

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
3920891

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sao:SAOUHSC_02517

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|93061.5.peg.2271

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA Translation: ABD31533.1
RefSeqiWP_000838485.1, NZ_LS483365.1
YP_500982.1, NC_007795.1

3D structure databases

SMRiQ2FW03
ModBaseiSearch...

Protein-protein interaction databases

STRINGi1280.SAXN108_2503

Genome annotation databases

EnsemblBacteriaiABD31533; ABD31533; SAOUHSC_02517
GeneIDi3920891
KEGGisao:SAOUHSC_02517
PATRICifig|93061.5.peg.2271

Phylogenomic databases

eggNOGiCOG0550, Bacteria
COG0551, Bacteria
HOGENOMiCLU_002929_5_2_9
OMAiTYPRVDT

Family and domain databases

CDDicd00186, TOP1Ac, 1 hit
cd03362, TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit
HAMAPiMF_00953, Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR005738, TopoIII
IPR006171, TOPRIM_domain
IPR034144, TOPRIM_TopoIII
PANTHERiPTHR11390, PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit
PRINTSiPR00417, PRTPISMRASEI
SMARTiView protein in SMART
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit
SUPFAMiSSF56712, SSF56712, 1 hit
TIGRFAMsiTIGR01056, topB, 1 hit
PROSITEiView protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOP3_STAA8
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q2FW03
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 21, 2006
Last modified: June 2, 2021
This is version 94 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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