UniProtKB - Q2FEF1 (IDI2_STAA3)
Protein
Isopentenyl-diphosphate delta-isomerase
Gene
fni
Organism
Staphylococcus aureus (strain USA300)
Status
Functioni
Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).UniRule annotation
Catalytic activityi
- EC:5.3.3.2UniRule annotation
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | FMN; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 124 | FMNUniRule annotation | 1 | |
Binding sitei | 154 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 155 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 186 | FMNUniRule annotation | 1 | |
Binding sitei | 211 | FMNUniRule annotation | 1 | |
Binding sitei | 216 | FMN; via amide nitrogenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 65 – 67 | FMNUniRule annotation | 3 | |
Nucleotide bindingi | 262 – 264 | FMNUniRule annotation | 3 | |
Nucleotide bindingi | 283 – 284 | FMNUniRule annotation | 2 |
GO - Molecular functioni
- FMN binding Source: UniProtKB-UniRule
- isopentenyl-diphosphate delta-isomerase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- NADPH binding Source: UniProtKB-UniRule
- oxidoreductase activity Source: InterPro
GO - Biological processi
- isoprenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Isomerase |
Biological process | Isoprene biosynthesis |
Ligand | Flavoprotein, FMN, Magnesium, Metal-binding, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Isopentenyl-diphosphate delta-isomeraseUniRule annotation (EC:5.3.3.2UniRule annotation)Short name: IPP isomeraseUniRule annotation Alternative name(s): Isopentenyl diphosphate:dimethylallyl diphosphate isomeraseUniRule annotation Isopentenyl pyrophosphate isomeraseUniRule annotation Type 2 isopentenyl diphosphate isomeraseUniRule annotation Short name: IDI-2UniRule annotation |
Gene namesi | Name:fniUniRule annotation Ordered Locus Names:SAUSA300_2292 |
Organismi | Staphylococcus aureus (strain USA300) |
Taxonomic identifieri | 367830 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000048458 | 1 – 349 | Isopentenyl-diphosphate delta-isomeraseAdd BLAST | 349 |
Proteomic databases
PRIDEi | Q2FEF1 |
Interactioni
Subunit structurei
Homooctamer. Dimer of tetramers.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 10 | Substrate bindingUniRule annotation | 2 | |
Regioni | 95 – 97 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the IPP isomerase type 2 family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_065515_0_0_9 |
OMAi | WDWGIPT |
Family and domain databases
CDDi | cd02811, IDI-2_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00354, Idi_2, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000262, FMN-dep_DH IPR011179, IPdP_isomerase |
PANTHERi | PTHR43665, PTHR43665, 1 hit |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF003314, IPP_isomerase, 1 hit |
TIGRFAMsi | TIGR02151, IPP_isom_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q2FEF1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDFQREQRK NEHVEIAMAQ SDAMHSDFDK MRFVHHSIPS INVNDIDLTS
60 70 80 90 100
QTPDLTMAYP VYINAMTGGS EWTKNINEKL AVVARETGLA MAVGSTHAAL
110 120 130 140 150
RNPRMAETFT IARKMNPEGM IFSNVGADVP VEKALEAVEL LEAQALQIHV
160 170 180 190 200
NSPQELVMPE GNREFVTWLD NIASIVSRVS VPVIIKEVGF GMSKELMHDL
210 220 230 240 250
QQIGVKYVDV SGKGGTNFVD IENERRANKD MDYLSSWGQS TVESLLETTA
260 270 280 290 300
YQSEISVFAS GGLRTPLDAI KSLALGAKAT GMSRPFLNQV ENNGIAHTVA
310 320 330 340
YVESFIEHMK SIMTMLDAKN IDDLTQKQIV FSPEILSWIE QRNLNIHRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000255 Genomic DNA Translation: ABD20739.1 |
RefSeqi | WP_001279381.1, NZ_CP027476.1 |
Genome annotation databases
EnsemblBacteriai | ABD20739; ABD20739; SAUSA300_2292 |
KEGGi | saa:SAUSA300_2292 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000255 Genomic DNA Translation: ABD20739.1 |
RefSeqi | WP_001279381.1, NZ_CP027476.1 |
3D structure databases
SMRi | Q2FEF1 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q2FEF1 |
Genome annotation databases
EnsemblBacteriai | ABD20739; ABD20739; SAUSA300_2292 |
KEGGi | saa:SAUSA300_2292 |
Phylogenomic databases
HOGENOMi | CLU_065515_0_0_9 |
OMAi | WDWGIPT |
Family and domain databases
CDDi | cd02811, IDI-2_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00354, Idi_2, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000262, FMN-dep_DH IPR011179, IPdP_isomerase |
PANTHERi | PTHR43665, PTHR43665, 1 hit |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF003314, IPP_isomerase, 1 hit |
TIGRFAMsi | TIGR02151, IPP_isom_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IDI2_STAA3 | |
Accessioni | Q2FEF1Primary (citable) accession number: Q2FEF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | March 21, 2006 | |
Last modified: | December 2, 2020 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |