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Protein

MHC class I polypeptide-related sequence B

Gene

MICB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.3 Publications

GO - Molecular functioni

  • natural killer cell lectin-like receptor binding Source: UniProtKB

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • gamma-delta T cell activation Source: UniProtKB
  • immune response-activating cell surface receptor signaling pathway Source: UniProtKB
  • natural killer cell mediated cytotoxicity Source: GO_Central
  • negative regulation of defense response to virus by host Source: UniProtKB
  • regulation of immune response Source: Reactome
  • response to heat Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
  • response to retinoic acid Source: UniProtKB
  • T cell mediated cytotoxicity Source: GO_Central
  • viral process Source: UniProtKB-KW

Keywordsi

Biological processAdaptive immunity, Cytolysis, Host-virus interaction, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Names & Taxonomyi

Protein namesi
Recommended name:
MHC class I polypeptide-related sequence B
Short name:
MIC-B
Gene namesi
Name:MICBImported
Synonyms:PERB11.2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204516.9
HGNCiHGNC:7091 MICB
MIMi602436 gene
neXtProtiNX_Q29980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 309ExtracellularSequence analysisAdd BLAST287
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Topological domaini331 – 383CytoplasmicSequence analysisAdd BLAST53

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Rheumatoid arthritis (RA)1 Publication
Disease susceptibility is associated with variations affecting the gene represented in this entry. The MICB*004 allele is associated with rheumatoid arthritis.
Disease descriptionAn inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
See also OMIM:180300
Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk.

Organism-specific databases

DisGeNETi4277
MIMi180300 phenotype
PharmGKBiPA30812

Polymorphism and mutation databases

BioMutaiMICB
DMDMi74706857

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000034141823 – 383MHC class I polypeptide-related sequence BSequence analysisAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi119 ↔ 187PROSITE-ProRule annotation1 Publication
Glycosylationi164N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi225 ↔ 282PROSITE-ProRule annotation1 Publication
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Proteolytically cleaved and released from the cell surface of tumor cells.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ29980
PaxDbiQ29980
PeptideAtlasiQ29980
PRIDEiQ29980
ProteomicsDBi61282
61283 [Q29980-3]

PTM databases

iPTMnetiQ29980
PhosphoSitePlusiQ29980
SwissPalmiQ29980

Expressioni

Tissue specificityi

Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue.3 Publications

Inductioni

By heat shock, oxidative stress, retinoic acid, IFN-alpha and the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine. Induction by IFN-alpha is impaired in patients with chronic hepatitis C virus infection. Down-regulated by human cytomegalovirus UL112 microRNA during viral infection which leads to decreased binding of KLRK1/NKG2D and reduced killing by natural killer cells.6 Publications

Gene expression databases

BgeeiENSG00000204516
ExpressionAtlasiQ29980 baseline and differential
GenevisibleiQ29980 HS

Organism-specific databases

HPAiHPA064618

Interactioni

Subunit structurei

Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICB while HCST acts as an adapter molecule which enables signal transduction. Receptor-ligand interaction induces clustering of both proteins in ordered structures called immune synapses and also leads to their intercellular transfer. This is associated with a reduction in the cytotoxicity of KLRK1-expressing cells.By similarity4 Publications
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 glycoprotein UL16; this interaction causes sequestration of MICB in the endoplasmic reticulum and increases resistance to KLRK1-mediated cytotoxicity.1 Publication

GO - Molecular functioni

  • natural killer cell lectin-like receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110424, 45 interactors
CORUMiQ29980
STRINGi9606.ENSP00000252229

Structurei

Secondary structure

1383
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 37Combined sources12
Beta strandi46 – 51Combined sources6
Beta strandi54 – 60Combined sources7
Turni61 – 64Combined sources4
Beta strandi65 – 68Combined sources4
Helixi71 – 75Combined sources5
Helixi80 – 101Combined sources22
Beta strandi104 – 106Combined sources3
Beta strandi109 – 121Combined sources13
Beta strandi123 – 125Combined sources3
Beta strandi127 – 135Combined sources9
Beta strandi138 – 144Combined sources7
Turni145 – 147Combined sources3
Helixi156 – 170Combined sources15
Helixi176 – 197Combined sources22
Helixi201 – 203Combined sources3
Beta strandi208 – 214Combined sources7
Beta strandi218 – 233Combined sources16
Beta strandi236 – 241Combined sources6
Beta strandi250 – 253Combined sources4
Beta strandi264 – 273Combined sources10
Helixi277 – 279Combined sources3
Beta strandi280 – 286Combined sources7
Beta strandi289 – 294Combined sources6

3D structure databases

ProteinModelPortaliQ29980
SMRiQ29980
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ29980

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 298Ig-like C1-typeSequence analysisAdd BLAST92

Sequence similaritiesi

Belongs to the MHC class I family. MIC subfamily.Sequence analysis

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J8TZ Eukaryota
ENOG41118IQ LUCA
HOGENOMiHOG000231246
HOVERGENiHBG106364
InParanoidiQ29980
KOiK07985
OrthoDBiEOG091G0ANY
PhylomeDBiQ29980
TreeFamiTF342166

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.30.500.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003597 Ig_C1-set
IPR011161 MHC_I-like_Ag-recog
IPR037055 MHC_I-like_Ag-recog_sf
IPR011162 MHC_I/II-like_Ag-recog
PfamiView protein in Pfam
PF07654 C1-set, 1 hit
PF00129 MHC_I, 1 hit
SMARTiView protein in SMART
SM00407 IGc1, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF54452 SSF54452, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q29980-1) [UniParc]FASTAAdd to basket
Also known as: MICB11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLGRVLLFL AVAFPFAPPA AAAEPHSLRY NLMVLSQDES VQSGFLAEGH
60 70 80 90 100
LDGQPFLRYD RQKRRAKPQG QWAEDVLGAK TWDTETEDLT ENGQDLRRTL
110 120 130 140 150
THIKDQKGGL HSLQEIRVCE IHEDSSTRGS RHFYYDGELF LSQNLETQES
160 170 180 190 200
TVPQSSRAQT LAMNVTNFWK EDAMKTKTHY RAMQADCLQK LQRYLKSGVA
210 220 230 240 250
IRRTVPPMVN VTCSEVSEGN ITVTCRASSF YPRNITLTWR QDGVSLSHNT
260 270 280 290 300
QQWGDVLPDG NGTYQTWVAT RIRQGEEQRF TCYMEHSGNH GTHPVPSGKV
310 320 330 340 350
LVLQSQRTDF PYVSAAMPCF VIIIILCVPC CKKKTSAAEG PELVSLQVLD
360 370 380
QHPVGTGDHR DAAQLGFQPL MSATGSTGST EGA
Length:383
Mass (Da):42,646
Last modified:November 1, 1996 - v1
Checksum:i4291DAA55583F9DA
GO
Isoform 21 Publication (identifier: Q29980-2) [UniParc]FASTAAdd to basket
Also known as: MICB21 Publication, ex3-del

The sequence of this isoform differs from the canonical sequence as follows:
     109-151: Missing.

Show »
Length:340
Mass (Da):37,651
Checksum:i3910DFA1B654C555
GO
Isoform 31 Publication (identifier: Q29980-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-41: EPHSLRYNLMVLSQDESV → VEMGFHRVSQDGLDLLTS
     42-383: Missing.

Note: A GC to AG nucleotide substitution in intron 1 generates a splice junction which gives rise to an additional exon between exons 1 and 2. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:41
Mass (Da):4,287
Checksum:iF092DA8237AFA871
GO

Polymorphismi

The following alleles of MICB are known: MICB*001, MICB*002, MICB*003, MICB*004, MICB*005, MICB*006, MICB*007, MICB*008, MICB*009N, MICB*010, MICB*011, MICB*012, MICB*013, MICB*014, MICB*015, MICB*016, MICB*018, MICB*019, MICB*020, MICB*021N and MICB*022. MICB*009N and MICB*021N are null alleles which are not expressed. The sequence shown is that of MICB*001.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04406839E → G in allele MICB*002, allele MICB*003, allele MICB*004, allele MICB*005, allele MICB*006, allele MICB*007, allele MICB*008, allele MICB*010, allele MICB*011, allele MICB*012, allele MICB*013, allele MICB*014, allele MICB*015, allele MICB*016, allele MICB*018, allele MICB*019, allele MICB*020 and allele MICB*022. 2 PublicationsCorresponds to variant dbSNP:rs45578846Ensembl.1
Natural variantiVAR_04406968P → H in allele MICB*011. Corresponds to variant dbSNP:rs45583740Ensembl.1
Natural variantiVAR_04407075D → N in allele MICB*004 and allele MICB*020. Corresponds to variant dbSNP:rs3131639Ensembl.1
Natural variantiVAR_04407180K → E in allele MICB*002, allele MICB*007, allele MICB*008, allele MICB*014, allele MICB*015, allele MICB*016, allele MICB*019 and allele MICB*022. 2 PublicationsCorresponds to variant dbSNP:rs1065075Ensembl.1
Natural variantiVAR_04407288D → G in allele MICB*022. Corresponds to variant dbSNP:rs45486091Ensembl.1
Natural variantiVAR_044073105D → G in allele MICB*012. Corresponds to variant dbSNP:rs45502297Ensembl.1
Natural variantiVAR_044074121I → M in allele MICB*008. Corresponds to variant dbSNP:rs3134900Ensembl.1
Natural variantiVAR_059527136D → H. Corresponds to variant dbSNP:rs1051788Ensembl.1
Natural variantiVAR_044075136D → N in allele MICB*002, allele MICB*007, allele MICB*008, allele MICB*014, allele MICB*015, allele MICB*018, allele MICB*020 and allele MICB*022. 1 PublicationCorresponds to variant dbSNP:rs1051788Ensembl.1
Natural variantiVAR_044076212T → I in allele MICB*003. Corresponds to variant dbSNP:rs41293883Ensembl.1
Natural variantiVAR_044077215E → K in allele MICB*006 and allele MICB*015. Corresponds to variant dbSNP:rs45624537Ensembl.1
Natural variantiVAR_044078279R → K in allele MICB*007. Corresponds to variant dbSNP:rs45587032Ensembl.1
Natural variantiVAR_044079291G → S in allele MICB*013, allele MICB*014, allele MICB*015 and allele MICB*016. Corresponds to variant dbSNP:rs41273040Ensembl.1
Natural variantiVAR_044080300V → A in allele MICB*002, allele MICB*003, allele MICB*004, allele MICB*005, allele MICB*006, allele MICB*007, allele MICB*008, allele MICB*010, allele MICB*011, allele MICB*012, allele MICB*013, allele MICB*014 and allele MICB*015. 2 PublicationsCorresponds to variant dbSNP:rs45470602Ensembl.1
Natural variantiVAR_044081383A → T in allele MICB*003, allele MICB*004, allele MICB*010 and allele MICB*011. Corresponds to variant dbSNP:rs1065076Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05280124 – 41EPHSL…QDESV → VEMGFHRVSQDGLDLLTS in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_05280242 – 383Missing in isoform 3. 1 PublicationAdd BLAST342
Alternative sequenceiVSP_055246109 – 151Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91625 mRNA Translation: CAA62823.1
U65416 Genomic DNA Translation: AAB42011.1
AY885251 mRNA No translation available.
EF051579 Genomic DNA Translation: ABO16470.1
EF051580 Genomic DNA Translation: ABO16471.1
AK314228 mRNA Translation: BAG36899.1
BA000025 Genomic DNA Translation: BAB63307.1
AL662866 Genomic DNA Translation: CAI17555.1
AL663061 Genomic DNA Translation: CAI18082.1
BX001040 Genomic DNA Translation: CAI18629.1
BX005439 Genomic DNA Translation: CAI18747.1
CR753820 Genomic DNA Translation: CAQ07170.1
CR753864 Genomic DNA Translation: CAQ10628.1
CR788288 Genomic DNA Translation: CAQ09224.1
BC044218 mRNA Translation: AAH44218.1
U69978 Genomic DNA Translation: AAB51802.1
U95729 mRNA Translation: AAB71642.1
U95730 mRNA Translation: AAB71643.1
U95731 mRNA Translation: AAB71644.1
U95732 mRNA Translation: AAB71645.1
U95733 mRNA Translation: AAB71646.1
U95734 mRNA Translation: AAB71647.1
AJ563706 Genomic DNA Translation: CAD91554.1
AF021221 mRNA Translation: AAC39846.1
AF021222 mRNA Translation: AAC39847.1
AF021223 mRNA Translation: AAC39848.1
AF021224 mRNA Translation: AAC39849.1
AF021225 mRNA Translation: AAC39850.1
AF021226 mRNA Translation: AAC39851.1
AB003609 Genomic DNA Translation: BAA23476.1
AB003610 Genomic DNA Translation: BAA23477.1
AB003611 Genomic DNA Translation: BAA23478.1
AB003612 Genomic DNA Translation: BAA23479.1
AB003613 Genomic DNA Translation: BAA23480.1
AB003614 Genomic DNA Translation: BAA23481.1
AB003615 Genomic DNA Translation: BAA23482.1
AB003616 Genomic DNA Translation: BAA23483.1
AB003617 Genomic DNA Translation: BAA23484.1
AJ251156, AJ251157 Genomic DNA Translation: CAB72101.1
AJ251158, AJ251159 Genomic DNA Translation: CAB72097.1
AJ251160, AJ251161 Genomic DNA Translation: CAB72098.1
AJ606906 Genomic DNA Translation: CAE54932.1
AJ606907 Genomic DNA Translation: CAE54933.1
AJ606908 Genomic DNA Translation: CAE54934.1
AJ606909 Genomic DNA Translation: CAE54935.1
AJ606910 Genomic DNA Translation: CAE54936.1
AJ606911 Genomic DNA Translation: CAE54937.1
AJ606912 Genomic DNA Translation: CAE54938.1
AJ606913 Genomic DNA Translation: CAE54939.1
AJ606914 Genomic DNA Translation: CAE54940.1
AJ606915 Genomic DNA Translation: CAE54941.1
AJ606916 Genomic DNA Translation: CAE54942.1
AJ606917 Genomic DNA Translation: CAE54943.1
AJ606918 Genomic DNA Translation: CAE54944.1
AJ606919 Genomic DNA Translation: CAE54945.1
AJ606920 Genomic DNA Translation: CAE54946.1
AJ606921 Genomic DNA Translation: CAE54947.1
AJ606922 Genomic DNA Translation: CAE54948.1
AJ606923 Genomic DNA Translation: CAE54949.1
AJ606929 Genomic DNA Translation: CAE54955.1
AJ606930 Genomic DNA Translation: CAE54956.1
AJ606931 Genomic DNA Translation: CAE54957.1
RefSeqiNP_001276089.1, NM_001289160.1
NP_001276090.1, NM_001289161.1
NP_005922.2, NM_005931.4
UniGeneiHs.731446

Genome annotation databases

EnsembliENST00000399150; ENSP00000382103; ENSG00000204516
ENST00000427115; ENSP00000395391; ENSG00000204516
ENST00000428416; ENSP00000398412; ENSG00000206449
ENST00000436531; ENSP00000409414; ENSG00000238289
ENST00000436655; ENSP00000402484; ENSG00000238289
ENST00000438954; ENSP00000398212; ENSG00000234218
ENST00000443156; ENSP00000393355; ENSG00000227772
ENST00000546706; ENSP00000449672; ENSG00000231179
ENST00000551608; ENSP00000447696; ENSG00000224378
GeneIDi4277
KEGGihsa:4277
UCSCiuc003nto.6 human [Q29980-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMICB_HUMAN
AccessioniPrimary (citable) accession number: Q29980
Secondary accession number(s): A2AC57
, A6NP85, B0UZ10, B2RAK2, O14499, O14500, O19798, O19799, O19800, O19801, O19802, O19803, O78099, O78100, O78101, O78102, O78103, O78104, P79525, P79541, Q5GR31, Q5GR37, Q5GR41, Q5GR42, Q5GR43, Q5GR44, Q5GR46, Q5GR48, Q5RIY6, Q5SSK1, Q5ST25, Q7JK51, Q7YQ89, Q861E6, Q9MY18, Q9MY19, Q9MY20, Q9UBH4, Q9UBZ8, Q9UEJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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