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Protein

Prostacyclin synthase

Gene

PTGIS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).2 Publications

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106SubstrateBy similarity1
Binding sitei112Substrate; via amide nitrogenBy similarity1
Binding sitei287SubstrateBy similarity1
Binding sitei382SubstrateBy similarity1
Metal bindingi441Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-BTA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-BTA-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-BTA-197264 Nicotinamide salvaging
R-BTA-211979 Eicosanoids
R-BTA-211994 Sterols are 12-hydroxylated by CYP8B1
R-BTA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase1 Publication (EC:5.3.99.42 Publications)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:PTGIS
Synonyms:CYP8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Organism-specific databases

VGNCiVGNC:33509 PTGIS

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 20HelicalSequence analysisAdd BLAST20

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519091 – 500Prostacyclin synthaseAdd BLAST500

Proteomic databases

PaxDbiQ29626
PRIDEiQ29626

Expressioni

Gene expression databases

BgeeiENSBTAG00000017537

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023313

Structurei

3D structure databases

ProteinModelPortaliQ29626
SMRiQ29626
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni358 – 359Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00550000074551
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiQ29626
KOiK01831
OMAiVFHTFRQ
OrthoDBiEOG091G0767
TreeFamiTF105090

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR027286 PTGIS
PANTHERiPTHR24306:SF4 PTHR24306:SF4, 1 hit
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PIRSF500628 PTGIS, 1 hit
PRINTSiPR00465 EP450IV
SUPFAMiSSF48264 SSF48264, 1 hit

Sequencei

Sequence statusi: Complete.

Q29626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAVVFGLL AALLLLLLLT RRRTRRPGEP PLDLGSIPWL GHALEFGKDA
60 70 80 90 100
AGFLTRMKEK HGDIFTVLVG GRHVTVLLDP HSYDAVVWEP RSRLDFHAYA
110 120 130 140 150
VFLMERIFDV QLPHYNPGDE KSKMKPTLLH KELQALTDAM YTNLRTVLLG
160 170 180 190 200
DTVEAGSGWH EMGLLEFSYG FLLRAGYLTQ YGVEAPPHTQ ESQAQDRVHS
210 220 230 240 250
ADVFHTFRQL DLLLPKLARG SLSAGDKDRV GKVKGRLWKL LSPTRLASRA
260 270 280 290 300
HRSRWLESYL LHLEEMGVSE EMQARALVLQ LWATQGNMGP AAFWLLLFLL
310 320 330 340 350
KNPEALAAVR GELETVLLGA EQPISQMTTL PQKVLDSMPV LDSVLSESLR
360 370 380 390 400
LTAAPFITRE VVADLALPMA DGREFSLRRG DRLLLFPFLS PQKDPEIYTD
410 420 430 440 450
PEVFKYNRFL NPDGSEKKDF YKDGKRLKNY SLPWGAGHNQ CLGKGYAVNS
460 470 480 490 500
IKQFVFLVLT QFDLELITPD VDIPEFDLSR YGFGLMQPEH DVPVRYRIRP
Length:500
Mass (Da):56,629
Last modified:November 1, 1996 - v1
Checksum:i3728EEFEAE852403
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192S → N in AAA53674 (PubMed:8074709).Curated1
Sequence conflicti353A → P in AAA53674 (PubMed:8074709).Curated1
Sequence conflicti407N → T in AAA53674 (PubMed:8074709).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30718 mRNA Translation: BAA06383.1
L34208 mRNA Translation: AAA53674.1
BC120087 mRNA Translation: AAI20088.1
S67757 mRNA Translation: AAB29680.1
PIRiA53658
RefSeqiNP_776869.1, NM_174444.1
UniGeneiBt.5247

Genome annotation databases

EnsembliENSBTAT00000023313; ENSBTAP00000023313; ENSBTAG00000017537
GeneIDi282021
KEGGibta:282021

Similar proteinsi

Entry informationi

Entry nameiPTGIS_BOVIN
AccessioniPrimary (citable) accession number: Q29626
Secondary accession number(s): Q0P5G0, Q28841
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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