UniProtKB - Q29513 (GNMT_RABIT)
Protein
Glycine N-methyltransferase
Gene
GNMT
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Catalytic activityi
- EC:2.1.1.20
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 20 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 29 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 32 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 39 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 63 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 84 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 137 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 139 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 176 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 220 | SubstratePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- folic acid binding Source: UniProtKB-KW
- glycine binding Source: UniProtKB
- glycine N-methyltransferase activity Source: UniProtKB
GO - Biological processi
- S-adenosylmethionine metabolic process Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | Folate-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
SABIO-RKi | Q29513 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GNMT |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Other locations
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087527 | ‹1 – 292 | Glycine N-methyltransferaseAdd BLAST | ›292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | PhosphoserineBy similarity | 1 | |
Modified residuei | 32 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 44 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 190 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 195 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 200 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q29513 |
Expressioni
Tissue specificityi
Abundant in liver.
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000003341 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 116 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG410IF1C Eukaryota ENOG410ZWXN LUCA |
HOGENOMi | HOG000276537 |
InParanoidi | Q29513 |
Family and domain databases
InterProi | View protein in InterPro IPR014369 Gly/Sar_N_MeTrfase IPR041698 Methyltransf_25 IPR029063 SAM-dependent_MTases |
PANTHERi | PTHR16458 PTHR16458, 1 hit |
Pfami | View protein in Pfam PF13649 Methyltransf_25, 1 hit |
PIRSFi | PIRSF000385 Gly_N-mtase, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51600 SAM_GNMT, 1 hit |
i Sequence
Sequence statusi: Fragment.
Q29513-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL
60 70 80 90 100
LRQHGCRRVL DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR
110 120 130 140 150
RHEPAFDKWV IEEANWMTLD KDVPQPAGGG FDAVICLGNS FAHLPDCKGD
160 170 180 190 200
QSEHRQALKN IASMVRTGGL LVIDHRNYDH ILSTLCAPGK NIYYKSDLTK
210 220 230 240 250
DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL SYYPHCLAPF
260 270 280 290
TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13307 mRNA Translation: BAA02567.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13307 mRNA Translation: BAA02567.1 |
3D structure databases
SMRi | Q29513 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000003341 |
Proteomic databases
PRIDEi | Q29513 |
Phylogenomic databases
eggNOGi | ENOG410IF1C Eukaryota ENOG410ZWXN LUCA |
HOGENOMi | HOG000276537 |
InParanoidi | Q29513 |
Enzyme and pathway databases
SABIO-RKi | Q29513 |
Family and domain databases
InterProi | View protein in InterPro IPR014369 Gly/Sar_N_MeTrfase IPR041698 Methyltransf_25 IPR029063 SAM-dependent_MTases |
PANTHERi | PTHR16458 PTHR16458, 1 hit |
Pfami | View protein in Pfam PF13649 Methyltransf_25, 1 hit |
PIRSFi | PIRSF000385 Gly_N-mtase, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51600 SAM_GNMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNMT_RABIT | |
Accessioni | Q29513Primary (citable) accession number: Q29513 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | September 18, 2019 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families