UniProtKB - Q29449 (AT8A1_BOVIN)
Probable phospholipid-transporting ATPase IA
ATP8A1
Functioni
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids (By similarity).
Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS) (By similarity).
The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane (By similarity).
Acts as aminophospholipid translocase at the plasma membrane in neuronal cells (By similarity).
By similarityCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).By similarity EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 409 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 409 | MagnesiumBy similarity | 1 | |
Metal bindingi | 411 | MagnesiumBy similarity | 1 | |
Binding sitei | 534 | ATPBy similarity | 1 | |
Metal bindingi | 786 | MagnesiumBy similarity | 1 | |
Metal bindingi | 790 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 409 – 411 | ATPBy similarity | 3 | |
Nucleotide bindingi | 726 – 733 | ATPSequence analysis | 8 | |
Nucleotide bindingi | 789 – 790 | ATPBy similarity | 2 | |
Nucleotide bindingi | 1080 – 1087 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- magnesium ion binding Source: InterPro
- phosphatidylserine flippase activity Source: UniProtKB
- phosphatidylserine floppase activity Source: RHEA
GO - Biological processi
- aminophospholipid translocation Source: UniProtKB
- phospholipid translocation Source: GO_Central
Keywordsi
Molecular function | Translocase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 7.6.2.1, 908 |
Protein family/group databases
TCDBi | 3.A.3.8.1, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Probable phospholipid-transporting ATPase IACurated (EC:7.6.2.1By similarity)Alternative name(s): ATPase class I type 8A member 1 Chromaffin granule ATPase II |
Gene namesi | Name:ATP8A1By similarity |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Plasma membrane
- Cell membrane By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- chromaffin granule membrane By similarity; Multi-pass membrane protein By similarity
- Cytoplasmic granule By similarity
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in cytoplasmic punctate structures and localizes to the plasma membrane (By similarity). Localizes to plasma membranes of red blood cells (By similarity).By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Golgi apparatus
- trans-Golgi network Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- chromaffin granule membrane Source: UniProtKB-SubCell
- cytoplasmic vesicle Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- organelle membrane Source: UniProtKB
- phospholipid-translocating ATPase complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 65 | CytoplasmicSequence analysisAdd BLAST | 65 | |
Transmembranei | 66 – 86 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 87 – 92 | ExtracellularSequence analysis | 6 | |
Transmembranei | 93 – 115 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 116 – 297 | CytoplasmicSequence analysisAdd BLAST | 182 | |
Transmembranei | 298 – 319 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 320 – 344 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 345 – 366 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 367 – 842 | CytoplasmicSequence analysisAdd BLAST | 476 | |
Transmembranei | 843 – 863 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 864 – 875 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 876 – 895 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 896 – 925 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 926 – 947 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 948 – 961 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 962 – 984 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 985 – 990 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 991 – 1011 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1012 – 1029 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 1030 – 1055 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 1056 – 1149 | CytoplasmicSequence analysisAdd BLAST | 94 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046359 | 1 – 1149 | Probable phospholipid-transporting ATPase IAAdd BLAST | 1149 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 25 | PhosphoserineBy similarity | 1 | |
Modified residuei | 28 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 29 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1111 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q29449 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit.
Interacts with TMEM30A to form a flippase complex; this complex forms an intermediate phosphoenzyme.
Interacts with TMEM30B; this interaction is reported conflictingly.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000014818 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q29449 |
OrthoDBi | 587717at2759 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPTMRRTVSE IRSRAEGYEK TDDVSEKTSL ADQEEIRTIF INQPQLTKFC
60 70 80 90 100
NNHVSTAKYN IITFLPRFLY SQFRRAANSF FLFIALLQQI PDVSPTGRYT
110 120 130 140 150
TLVPLLFILA VAAIKEIIED IKRHKADNAV NKKQTQVLRN GAWEIVHWEK
160 170 180 190 200
VNVGDIVIIK GKEYIPADTV LLSSSEPQAM CYIETSNLDG ETNLKIRQGL
210 220 230 240 250
PATSDIKDID SLMRLSGRIE CESPNRHLYD FVGNIRLDGR STVPLGADQI
260 270 280 290 300
LLRGAQLRNT QWVHGIVVYT GHDTKLMQNS TSPPLKLSNV ERITNVQILI
310 320 330 340 350
LFCILIAMSL VCSVGSAIWN RRHSGRDWYL NLNYGGANNF GLNFLTFIIL
360 370 380 390 400
FNNLIPISLL VTLEVVKFTQ AYFINWDLDM HYEPTDTAAM ARTSNLNVEL
410 420 430 440 450
GQVKYIFSDK TGTLTCNVMQ FKKCTIAGVA YGQNSQFGDE KTFSDSSLLE
460 470 480 490 500
NLQNNHPTAP IICEFLTMMA VCHTAVPERE GDKIIYQAAS PDEGALVRAA
510 520 530 540 550
KQLNFVFTGR TPDSVIIDSL GQEERYELLN VLEFTSARKR MSVIVRTPSG
560 570 580 590 600
KLRLYCKGAD TVIYDRLAET SKYKEITLKH LEQFATEGLR TLCFAVAEIS
610 620 630 640 650
ESDFQEWRAV YHRASTSVQN RLLKLEESYE LIEKNLQLLG ATAIEDKLQD
660 670 680 690 700
QVPETIETLM KADIKIWILT GDKQETAINI GHSCKLRRKN MGMIVINEGS
710 720 730 740 750
LDGTRETLSR HCTTLGDALR KENDFALIID GKTLKYALTF GVRQYFLDLA
760 770 780 790 800
LSCKAVICCR VSPLQKSEVV EMVKKQVKVI TLAIGDGAND VSMIQTAHVG
810 820 830 840 850
VGISGNEGLQ AANSSDYSIA QFKYLKNLLM VHGAWNYNRG SKCILYCFYK
860 870 880 890 900
NIVLYIIEIW FAFVNGFSGQ ILFERWCIGL YNVMFTAMPP LTLGIFERSC
910 920 930 940 950
RKEYMLKYPE LYKTSQNALD FNTKVFWVHC LNGLFHSVIL FWFPLKALQY
960 970 980 990 1000
GTVFENGRTS DYLLLGNFVY TFVVITVCLK AGLETSYWTW FSHIAIWGSI
1010 1020 1030 1040 1050
ALWVVFFGIY SSLWPAVPMA PDMSGEAAML FSSGVFWMGL LFIPVASLLL
1060 1070 1080 1090 1100
DVVYKVIKRT AFKTLVDEVQ ELEAKSQDPG AVVLGKSLTE RAQLLKNVFK
1110 1120 1130 1140
KNHVNLYRSE SLQQNLLHGY AFSQDENGIV SQSEVIRAYD TTKQRPDEW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51100 mRNA Translation: AAD03352.1 |
PIRi | T18515 |
RefSeqi | NP_777263.1, NM_174838.2 |
Genome annotation databases
GeneIDi | 317692 |
KEGGi | bta:317692 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51100 mRNA Translation: AAD03352.1 |
PIRi | T18515 |
RefSeqi | NP_777263.1, NM_174838.2 |
3D structure databases
AlphaFoldDBi | Q29449 |
SMRi | Q29449 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000014818 |
Protein family/group databases
TCDBi | 3.A.3.8.1, the p-type atpase (p-atpase) superfamily |
Proteomic databases
PRIDEi | Q29449 |
Genome annotation databases
GeneIDi | 317692 |
KEGGi | bta:317692 |
Organism-specific databases
CTDi | 10396 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q29449 |
OrthoDBi | 587717at2759 |
Enzyme and pathway databases
BRENDAi | 7.6.2.1, 908 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT8A1_BOVIN | |
Accessioni | Q29449Primary (citable) accession number: Q29449 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families