UniProtKB - Q29371 (TPIS_PIG)
Protein
Triosephosphate isomerase
Gene
TPI1
Organism
Sus scrofa (Pig)
Status
Functioni
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.By similarity
It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.By similarity
Catalytic activityi
: glycolysis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.PROSITE-ProRule annotationProteins known to be involved in this subpathway in this organism are:
- Triosephosphate isomerase, Triosephosphate isomerase (TPI1), Triosephosphate isomerase (TPI1), Triosephosphate isomerase (TPI1), Triosephosphate isomerase (TPI1), Triosephosphate isomerase, Triosephosphate isomerase (TPI1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Pathwayi: gluconeogenesis
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 14 | SubstratePROSITE-ProRule annotation | 1 | |
Active sitei | 96 | ElectrophilePROSITE-ProRule annotation | 1 | |
Active sitei | 166 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- methylglyoxal synthase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- triose-phosphate isomerase activity Source: UniProtKB
GO - Biological processi
- gluconeogenesis Source: GO_Central
- glyceraldehyde-3-phosphate biosynthetic process Source: UniProtKB
- glycerol catabolic process Source: GO_Central
- glycolytic process Source: GO_Central
- methylglyoxal biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase, Lyase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TPI1 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000090118 | 2 – 248 | Triosephosphate isomeraseAdd BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 68 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 106 | PhosphoserineBy similarity | 1 | |
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Modified residuei | 149 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 156 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 156 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 173 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 194 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 194 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 194 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 209 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 212 | PhosphoserineBy similarity | 1 | |
Modified residuei | 214 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 223 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q29371 |
PeptideAtlasi | Q29371 |
PRIDEi | Q29371 |
Interactioni
Subunit structurei
Homodimer.
PROSITE-ProRule annotationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000000730 |
Family & Domainsi
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.Curated
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
InParanoidi | Q29371 |
OrthoDBi | 1272577at2759 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q29371-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPARKFFVG GNWKMNGRKN NLGELINTLN AAKLPADTEV VCAPPTAYID
60 70 80 90 100
FARQKLDPKI AVAAQNCYKV ANGAFTGEIG PGMIKDLGAT WVVLGHSERR
110 120 130 140 150
HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV
160 170 180 190 200
IADNVKDWNK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKTHVPEA
210 220 230 240
VAHSTRIIYG GSVTGATCKE LASQPDVDGF RVSGASLKPE FVDIINAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 | G → S in CAA23243 (PubMed:8672129).Curated | 1 | |
Sequence conflicti | 85 | K → R in CAA23243 (PubMed:8672129).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ176425 mRNA Translation: ABA02206.1 F14774 mRNA Translation: CAA23243.1 |
RefSeqi | NP_001032228.1, NM_001037151.1 |
Genome annotation databases
GeneIDi | 100157582 |
KEGGi | ssc:100157582 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ176425 mRNA Translation: ABA02206.1 F14774 mRNA Translation: CAA23243.1 |
RefSeqi | NP_001032228.1, NM_001037151.1 |
3D structure databases
SMRi | Q29371 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000000730 |
Proteomic databases
PaxDbi | Q29371 |
PeptideAtlasi | Q29371 |
PRIDEi | Q29371 |
Genome annotation databases
GeneIDi | 100157582 |
KEGGi | ssc:100157582 |
Organism-specific databases
CTDi | 7167 |
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
InParanoidi | Q29371 |
OrthoDBi | 1272577at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_PIG | |
Accessioni | Q29371Primary (citable) accession number: Q29371 Secondary accession number(s): Q3S2V8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families