UniProtKB - Q28IU1 (DHB12_XENTR)
Very-long-chain 3-oxoacyl-CoA reductase
hsd17b12
Functioni
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
By similarityCatalytic activityi
- a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP+ = a very-long-chain 3-oxoacyl-CoA + H+ + NADPHBy similarityEC:1.1.1.330By similarity
- EC:1.1.1.62By similarity
- EC:1.1.1.62By similarity
- (8Z,11Z,14Z)-3-oxoeicosatrienoyl-CoA + H+ + NADPH = (3R)-hydroxy-(8Z,11Z,14Z)-eicosatrienoyl-CoA + NADP+By similarity
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Pathwayi: estrogen biosynthesis
This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 196 | SubstrateBy similarity | 1 | |
Active sitei | 209 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 56 – 85 | NADPBy similarityAdd BLAST | 30 |
GO - Molecular functioni
- 17-beta-hydroxysteroid dehydrogenase (NAD+) activity Source: RHEA
- 17-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: RHEA
- 3-oxo-arachidoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-behenoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-cerotoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-lignoceroyl-CoA reductase activity Source: UniProtKB-EC
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- estrogen biosynthetic process Source: UniProtKB-UniPathway
- fatty acid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
Reactomei | R-XTR-193048, Androgen biosynthesis R-XTR-75876, Synthesis of very long-chain fatty acyl-CoAs |
UniPathwayi | UPA00094 UPA00769 |
Names & Taxonomyi
Protein namesi | Recommended name: Very-long-chain 3-oxoacyl-CoA reductaseCurated (EC:1.1.1.330By similarity)Alternative name(s): 17-beta-hydroxysteroid dehydrogenase 12By similarity Short name: 17-beta-HSD 12By similarity 3-ketoacyl-CoA reductaseBy similarity Short name: KARBy similarity Estradiol 17-beta-dehydrogenase 12By similarity (EC:1.1.1.62By similarity) |
Gene namesi | Name:hsd17b12 ORF Names:TNeu053h21.1 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-945229, hsd17b12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 283 – 303 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248375 | 1 – 320 | Very-long-chain 3-oxoacyl-CoA reductaseAdd BLAST | 320 |
Proteomic databases
PaxDbi | Q28IU1 |
Expressioni
Gene expression databases
Bgeei | ENSXETG00000022229, Expressed in embryo and 15 other tissues |
ExpressionAtlasi | Q28IU1, baseline |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1014, Eukaryota |
GeneTreei | ENSGT00940000154860 |
HOGENOMi | CLU_010194_38_0_1 |
InParanoidi | Q28IU1 |
OMAi | HLEQRTT |
OrthoDBi | 895581at2759 |
PhylomeDBi | Q28IU1 |
TreeFami | TF314591 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MATESLAEVP VPGCNCFWYL GVVAAVWWGL RAAWCLLDGA RVWVLGSGAQ
60 70 80 90 100
VGPRIGKWAV VTGATDGIGK AYAEELAKRG MNIVLISRSP EKLEEVAKQI
110 120 130 140 150
KEKFKVETKI IAADFGKPTE IYGRIESGLR DLEIGVLVNN VGVSYEHPEY
160 170 180 190 200
FLEIPDLENT LDKMININIT SVCQMTRLVL PGMLGRGRGV ILNISSASGM
210 220 230 240 250
YPVPLLTVYS ATKAFVDFFS RGLQAEYRSK GVTVQSVLPF YVATKLAKIR
260 270 280 290 300
KPTWDKPSPE TYVQSALNTV GLQTQTNGYL PHAIMGWIST SLVPVSTAIS
310 320
LGMKMNKGLR ARFLKRAKQK
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB6ID06 | B6ID06_XENTR | Non-specific serine/threonine prote... | csnk1g3 hsd17b12 | 416 | Annotation score: | ||
A0A6I8PY01 | A0A6I8PY01_XENTR | Non-specific serine/threonine prote... | csnk1g3 | 448 | Annotation score: | ||
A0A6I8R6G6 | A0A6I8R6G6_XENTR | Non-specific serine/threonine prote... | csnk1g3 | 456 | Annotation score: | ||
A0A6I8QRW6 | A0A6I8QRW6_XENTR | Non-specific serine/threonine prote... | csnk1g3 | 414 | Annotation score: | ||
A0A6I8RUE1 | A0A6I8RUE1_XENTR | Non-specific serine/threonine prote... | csnk1g3 | 439 | Annotation score: | ||
A0A6I8SGS2 | A0A6I8SGS2_XENTR | Non-specific serine/threonine prote... | csnk1g3 | 411 | Annotation score: | ||
A0A6I8ST21 | A0A6I8ST21_XENTR | Casein kinase 1 gamma 3 | csnk1g3 | 385 | Annotation score: | ||
A0A6I8RBQ3 | A0A6I8RBQ3_XENTR | Very-long-chain 3-oxoacyl-CoA reduc... | hsd17b12 | 314 | Annotation score: | ||
A0A6I8QRP8 | A0A6I8QRP8_XENTR | Very-long-chain 3-oxoacyl-CoA reduc... | hsd17b12 | 298 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR760225 mRNA Translation: CAJ83357.1 |
RefSeqi | NP_001017234.1, NM_001017234.2 |
Genome annotation databases
Ensembli | ENSXETT00000048094; ENSXETP00000048094; ENSXETG00000022229 |
GeneIDi | 549988 |
KEGGi | xtr:549988 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR760225 mRNA Translation: CAJ83357.1 |
RefSeqi | NP_001017234.1, NM_001017234.2 |
3D structure databases
SMRi | Q28IU1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000048094 |
Proteomic databases
PaxDbi | Q28IU1 |
Genome annotation databases
Ensembli | ENSXETT00000048094; ENSXETP00000048094; ENSXETG00000022229 |
GeneIDi | 549988 |
KEGGi | xtr:549988 |
Organism-specific databases
CTDi | 51144 |
Xenbasei | XB-GENE-945229, hsd17b12 |
Phylogenomic databases
eggNOGi | KOG1014, Eukaryota |
GeneTreei | ENSGT00940000154860 |
HOGENOMi | CLU_010194_38_0_1 |
InParanoidi | Q28IU1 |
OMAi | HLEQRTT |
OrthoDBi | 895581at2759 |
PhylomeDBi | Q28IU1 |
TreeFami | TF314591 |
Enzyme and pathway databases
UniPathwayi | UPA00094 UPA00769 |
Reactomei | R-XTR-193048, Androgen biosynthesis R-XTR-75876, Synthesis of very long-chain fatty acyl-CoAs |
Gene expression databases
Bgeei | ENSXETG00000022229, Expressed in embryo and 15 other tissues |
ExpressionAtlasi | Q28IU1, baseline |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHB12_XENTR | |
Accessioni | Q28IU1Primary (citable) accession number: Q28IU1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | April 4, 2006 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families