UniProtKB - Q28GQ2 (MECR_XENTR)
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
mecr
Functioni
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro (By similarity).
Functions in pronephros development, regulating late differentiation of all pronephric tubule segments (PubMed:18472403).
By similarity1 PublicationCatalytic activityi
- EC:1.3.1.104By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 68 | Proton donorBy similarity | 1 | |
Binding sitei | 141 | NADPBy similarity | 1 | |
Binding sitei | 345 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 167 – 170 | NADPBy similarity | 4 | |
Nucleotide bindingi | 190 – 192 | NADPBy similarity | 3 | |
Nucleotide bindingi | 259 – 262 | NADPBy similarity | 4 | |
Nucleotide bindingi | 284 – 286 | NADPBy similarity | 3 |
GO - Molecular functioni
- trans-2-enoyl-CoA reductase (NADPH) activity Source: GO_Central
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
- fatty acid metabolic process Source: GO_Central
- pronephric nephron tubule development Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | NADP |
Enzyme and pathway databases
Reactomei | R-XTR-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Names & Taxonomyi
Protein namesi | Recommended name: Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104)Alternative name(s): 2-enoyl thioester reductase |
Gene namesi | Name:mecr ORF Names:TEgg103l11.1 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-1007523, mecr |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 12 | MitochondrionSequence analysisAdd BLAST | 12 | |
ChainiPRO_0000391692 | 13 – 350 | Enoyl-[acyl-carrier-protein] reductase, mitochondrialSequence analysisAdd BLAST | 338 |
Proteomic databases
PaxDbi | Q28GQ2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSXETG00000014371, Expressed in mesonephros and 25 other tissues |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000031439 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0025, Eukaryota |
GeneTreei | ENSGT00940000156592 |
HOGENOMi | CLU_026673_17_1_1 |
InParanoidi | Q28GQ2 |
OMAi | RNSKHTE |
OrthoDBi | 1269870at2759 |
PhylomeDBi | Q28GQ2 |
TreeFami | TF312886 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MWLGLRLFHR PFSSLAARGL VYEKHGEPLQ VLRLKNVNIT HPADNEVRVK
60 70 80 90 100
MLAAPINPSD INMVQGTYAL LPQLPAVGGN EGVGVVVEIG RHVSSMRPGD
110 120 130 140 150
WVVPVDAGLG TWCTEAVFSE DSLVRVPSDI PVAGAATVSV NPCTAYRLLS
160 170 180 190 200
DFETLRPGDT IIQNASNSGV GQAVIQIATS LGITTINVVR DREDLSSLIQ
210 220 230 240 250
RLRDLGADHV ITEEQLRKPE MKDLFKNCPR PRLALNCVGG KSTTEMLRHL
260 270 280 290 300
DYGGTMVTYG GMSKQPVTVP VSALIFKNVK LCGFWVTQWK KERAQTDREE
310 320 330 340 350
IVKMIRDLCD LIRRGKLVPP PSTQRPLEDF SRALQDSQTP FLSRKQILIM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8J0SCM1 | A0A8J0SCM1_XENTR | enoyl-[acyl-carrier-protein] reduct... | mecr cgi-63, fasn2b, nrbf1 | 300 | Annotation score: | ||
A0A8J0SFM8 | A0A8J0SFM8_XENTR | enoyl-[acyl-carrier-protein] reduct... | mecr cgi-63, fasn2b, nrbf1 | 325 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR761275 mRNA Translation: CAJ83709.1 |
RefSeqi | NP_001016371.1, NM_001016371.2 |
Genome annotation databases
GeneIDi | 549125 |
KEGGi | xtr:549125 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR761275 mRNA Translation: CAJ83709.1 |
RefSeqi | NP_001016371.1, NM_001016371.2 |
3D structure databases
AlphaFoldDBi | Q28GQ2 |
SMRi | Q28GQ2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000031439 |
Proteomic databases
PaxDbi | Q28GQ2 |
Genome annotation databases
GeneIDi | 549125 |
KEGGi | xtr:549125 |
Organism-specific databases
CTDi | 51102 |
Xenbasei | XB-GENE-1007523, mecr |
Phylogenomic databases
eggNOGi | KOG0025, Eukaryota |
GeneTreei | ENSGT00940000156592 |
HOGENOMi | CLU_026673_17_1_1 |
InParanoidi | Q28GQ2 |
OMAi | RNSKHTE |
OrthoDBi | 1269870at2759 |
PhylomeDBi | Q28GQ2 |
TreeFami | TF312886 |
Enzyme and pathway databases
Reactomei | R-XTR-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Gene expression databases
Bgeei | ENSXETG00000014371, Expressed in mesonephros and 25 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MECR_XENTR | |
Accessioni | Q28GQ2Primary (citable) accession number: Q28GQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 2, 2010 |
Last sequence update: | April 4, 2006 | |
Last modified: | May 25, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families