UniProtKB - Q28F67 (AATM_XENTR)
Protein
Aspartate aminotransferase, mitochondrial
Gene
got2
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism.By similarity
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 62 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 159 | SubstrateBy similarity | 1 | |
Binding sitei | 212 | SubstrateBy similarity | 1 | |
Binding sitei | 404 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate metabolic process Source: UniProtKB
- biosynthetic process Source: InterPro
- glutamate metabolic process Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-XTR-389661, Glyoxylate metabolism and glycine degradation R-XTR-70263, Gluconeogenesis R-XTR-8963693, Aspartate and asparagine metabolism R-XTR-8964539, Glutamate and glutamine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)Short name: mAspAT Alternative name(s): Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Transaminase A |
Gene namesi | Name:got2 ORF Names:TGas123d19.1 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | MitochondrionBy similarityAdd BLAST | 26 | |
ChainiPRO_0000327598 | 27 – 427 | Aspartate aminotransferase, mitochondrialAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | Q28F67 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000034675 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_0_1 |
InParanoidi | Q28F67 |
OrthoDBi | 1104596at2759 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q28F67-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLKSRLLV GVARCQPCLA AVQGRASSWW SHVEMGPPDP ILGVTEAFKR
60 70 80 90 100
DTNSKKMNLG VGAYRDDNGK PYVLSSVRKA EAQLASKNLD KEYLPIGGLA
110 120 130 140 150
EFARASAQLA LGENCDAIQS GRFITVQTIS GTGSLRVGAN FLQRFYKYSR
160 170 180 190 200
DVYLPKPSWG NHTPIFRDAG LEVKGYRYYD PKTCGFDFTG ALDDISKIPE
210 220 230 240 250
QSIILFHACA HNPTGVDPKQ EQWKELAALI KSRRLFPFFD MAYQGFASGD
260 270 280 290 300
TNRDAWAVRH FIQEGINVVL SQSYAKNMGL YGERVGAFTV VCSDAEEAKR
310 320 330 340 350
VESQLKILIR PMYSNPPLNG ARIAAAILTQ PDLRKEWLQE VKGMANRIIS
360 370 380 390 400
MREQLVSNLK KEGSIHNWQH ISDQIGMFCF TGLRPEQVER LIKEFSIYMT
410 420
KDGRISVAGV TSANNGYLAH AIHQVTK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A6I8QZK0 | A0A6I8QZK0_XENTR | Aspartate aminotransferase | got2 | 357 | Annotation score: | ||
F6YBV5 | F6YBV5_XENTR | Aspartate aminotransferase, mitocho... | got2 | 411 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR762138 mRNA Translation: CAJ83961.1 |
RefSeqi | NP_001016933.1, NM_001016933.2 |
Genome annotation databases
Ensembli | ENSXETT00000100393; ENSXETP00000087856; ENSXETG00000015896 |
GeneIDi | 549687 |
KEGGi | xtr:549687 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR762138 mRNA Translation: CAJ83961.1 |
RefSeqi | NP_001016933.1, NM_001016933.2 |
3D structure databases
SMRi | Q28F67 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000034675 |
Proteomic databases
PaxDbi | Q28F67 |
Genome annotation databases
Ensembli | ENSXETT00000100393; ENSXETP00000087856; ENSXETG00000015896 |
GeneIDi | 549687 |
KEGGi | xtr:549687 |
Organism-specific databases
CTDi | 2806 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_0_1 |
InParanoidi | Q28F67 |
OrthoDBi | 1104596at2759 |
Enzyme and pathway databases
Reactomei | R-XTR-389661, Glyoxylate metabolism and glycine degradation R-XTR-70263, Gluconeogenesis R-XTR-8963693, Aspartate and asparagine metabolism R-XTR-8964539, Glutamate and glutamine metabolism |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATM_XENTR | |
Accessioni | Q28F67Primary (citable) accession number: Q28F67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 4, 2006 | |
Last modified: | December 2, 2020 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families