UniProtKB - Q28E61 (NSUN2_XENTR)
RNA cytosine-C(5)-methyltransferase NSUN2
nsun2
Functioni
RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C5-methylation of mitochondrial tRNAs. Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay. Cytosine C5-methylation of mRNAs also regulates mRNA export. Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
By similarityCatalytic activityi
- cytidine48 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine48 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine49 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine49 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine50 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine50 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine34 in tRNA precursor + S-adenosyl-L-methionine = 5-methylcytidine34 in tRNA precursor + H+ + S-adenosyl-L-homocysteineBy similarityEC:2.1.1.203By similarityThis reaction proceeds in the forwardBy similarity direction.
- a cytidine in mRNA + S-adenosyl-L-methionine = a 5-methylcytidine in mRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 217 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 244 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 270 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 323 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- methyltransferase activity Source: GO_Central
- mRNA (cytidine-5-)-methyltransferase activity Source: UniProtKB
- tRNA (cytosine-5-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: GO_Central
GO - Biological processi
- mRNA methylation Source: UniProtKB
- regulation of mRNA export from nucleus Source: UniProtKB
- regulation of stem cell differentiation Source: UniProtKB
- RNA methylation Source: GO_Central
- tRNA methylation Source: UniProtKB
- tRNA stabilization Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: RNA cytosine-C(5)-methyltransferase NSUN2Curated (EC:2.1.1.-By similarity)Alternative name(s): |
Gene namesi | Name:nsun2By similarity ORF Names:TEgg048n02.11 Publication |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-962623, nsun2 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Nucleus
- nucleolus By similarity
Extracellular region or secreted
- extracellular exosome By similarity
Cytoskeleton
- spindle By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- spindle Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289227 | 1 – 798 | RNA cytosine-C(5)-methyltransferase NSUN2Add BLAST | 798 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q28E61 |
PRIDEi | Q28E61 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 186 – 192 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 7 | |
Regioni | 476 – 499 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 723 – 798 | DisorderedSequence analysisAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 30 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 485 – 499 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 723 – 751 | Basic and acidic residuesSequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2198, Eukaryota |
GeneTreei | ENSGT00940000153665 |
InParanoidi | Q28E61 |
OrthoDBi | 911098at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR023270, RCMT_NCL1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22808, PTHR22808, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY PR02011, RCMTNCL1 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGRRNRRNRQ RHQRSTEQRS PAEEEQRRKA REQAAWECGY PEIIKENKLF
60 70 80 90 100
EHYYQELKIV PDGEWDKFMA SLREPLPATI RITGYKSHAK EILHCLKEKY
110 120 130 140 150
FKELQDIEVD GQKIEAPQPL SWYPEELAWH TNLSRKIIRK SPELEKFHQF
160 170 180 190 200
LVNETESGNI SRQEAVSMIP PVLLKVQPHH KILDMCAAPG SKTAQIIEML
210 220 230 240 250
HADMNVPFPE GFVIANDVDN KRCYLLVHQA KRLNSPCIMV VNHDASSIPR
260 270 280 290 300
LLVENNGSRE VLYYDRILCD VPCSGDGTLR KNIDVWKKWT TLNSLQLHGL
310 320 330 340 350
QIRIATRGVE QLAEGGRMVY STCSLNPVED EAVIASLLDK SEGSLELADV
360 370 380 390 400
ASEIPGLKWM PGITQWKVMT KEGHWYEKWE DIPTSRHTQI RPTMFPPKDE
410 420 430 440 450
EKLKSMNLNR CMRILPHHQN TGGFFVAVLI KKAPMPWNKR QPKLQRRPPV
460 470 480 490 500
SACDASIAVA PELVKAVTEN SAGMADEPAV DTENGETKPC TNQSDSSKTD
510 520 530 540 550
IVCCPPPSKK MKLFGFKEDP FVFVSEDDPI FDPIQTFYAL DPSFPKKNLL
560 570 580 590 600
TRTQEGKKRQ LYMVSKELRN VLLHNSEKMK VINTGIKVLC RNNDGEQYGC
610 620 630 640 650
AYRLAQEGIY TLYPFINARI VTVSIEDIKV LLTQENPFLS KFSKETQKQA
660 670 680 690 700
NNFDMGSIVL KYEPDPQEPE TLQCPIVLCG WRGKTSIRSF VPKNERLHYL
710 720 730 740 750
RMMGVEVFKE KAEVLEKKPV EGKACDEEHI DEKMDIDGAK EESKELSGNE
760 770 780 790
SGDDEDPKEE DVIDRGVLEH VALKNTSAIP ASVEDQAEDA SVSKESVD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A6I8QL71 | A0A6I8QL71_XENTR | NOL1/NOP2/Sun domain family member ... | nsun2 | 775 | Annotation score: | ||
A0A6I8SLZ4 | A0A6I8SLZ4_XENTR | NOL1/NOP2/Sun domain family member ... | nsun2 | 771 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR848434 mRNA Translation: CAJ83692.1 |
RefSeqi | NP_001015962.1, NM_001015962.2 XP_012820119.1, XM_012964665.2 XP_012820120.1, XM_012964666.2 |
Genome annotation databases
Ensembli | ENSXETT00000062642; ENSXETP00000059424; ENSXETG00000001732 |
GeneIDi | 548716 |
KEGGi | xtr:548716 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR848434 mRNA Translation: CAJ83692.1 |
RefSeqi | NP_001015962.1, NM_001015962.2 XP_012820119.1, XM_012964665.2 XP_012820120.1, XM_012964666.2 |
3D structure databases
SMRi | Q28E61 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000059424 |
Proteomic databases
PaxDbi | Q28E61 |
PRIDEi | Q28E61 |
Genome annotation databases
Ensembli | ENSXETT00000062642; ENSXETP00000059424; ENSXETG00000001732 |
GeneIDi | 548716 |
KEGGi | xtr:548716 |
Organism-specific databases
CTDi | 54888 |
Xenbasei | XB-GENE-962623, nsun2 |
Phylogenomic databases
eggNOGi | KOG2198, Eukaryota |
GeneTreei | ENSGT00940000153665 |
InParanoidi | Q28E61 |
OrthoDBi | 911098at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR023270, RCMT_NCL1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22808, PTHR22808, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY PR02011, RCMTNCL1 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NSUN2_XENTR | |
Accessioni | Q28E61Primary (citable) accession number: Q28E61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | April 4, 2006 | |
Last modified: | February 23, 2022 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families