UniProtKB - Q28901 (F263_BOVIN)
Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Gene
PFKFB3
Organism
Bos taurus (Bovine)
Status
Functioni
Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 99 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 125 | Sequence analysis | 1 | |
Binding sitei | 127 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 133 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 155 | Sequence analysis | 1 | |
Binding sitei | 169 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 191 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 195 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 254 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 254 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 255 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 261 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 261 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 267 | Fructose 2,6-bisphosphate; via amide nitrogenBy similarity | 1 | |
Active sitei | 324 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 335 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 353 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 364 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 389 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 390 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 426 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 42 – 50 | ATPBy similarity | 9 | |
Nucleotide bindingi | 164 – 169 | ATPBy similarity | 6 | |
Nucleotide bindingi | 346 – 349 | ATPBy similarity | 4 | |
Nucleotide bindingi | 390 – 394 | ATPBy similarity | 5 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- fructose-2,6-bisphosphate 2-phosphatase activity Source: GO_Central
GO - Biological processi
- fructose 2,6-bisphosphate metabolic process Source: GO_Central
- fructose metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Short name: 6PF-2-K/Fru-2,6-P2ase 3 Short name: PFK/FBPase 3 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme Including the following 2 domains: Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
Gene namesi | Name:PFKFB3 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000179967 | 1 – ›463 | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3Add BLAST | ›463 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 462 | Phosphoserine; by AMPK and PKABy similarity | 1 |
Post-translational modificationi
Phosphorylation by AMPK stimulates activity.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q28901 |
PRIDEi | Q28901 |
Expressioni
Tissue specificityi
Brain.
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000048148 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 246 | 6-phosphofructo-2-kinaseAdd BLAST | 246 | |
Regioni | 247 – ›463 | Fructose-2,6-bisphosphataseAdd BLAST | ›217 |
Sequence similaritiesi
In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
InParanoidi | Q28901 |
OMAi | DMLAWFR |
OrthoDBi | 392001at2759 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
i Sequence
Sequence statusi: Fragment.
Q28901-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLELTQSRV QKIWIPVDHR PSLPRTCGPK LTNSPTVIVM VGLPARGKTY
60 70 80 90 100
ISKKLTRYLN WIGVPTKVFN LGEYRRDGVK QYSSYNFFRP DNEEAMKVRK
110 120 130 140 150
QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF PKENDFKVFF
160 170 180 190 200
IESVCDDPTV VASNIMEVKI SSPDYKDCNS RENAMDDFMK RINCYEASYQ
210 220 230 240 250
PLDPDNDDRD LSLIKVIDVG QRFLVNRVQD HIQRRIVYYL MNIHWQPRTI
260 270 280 290 300
YLCRHGESKH NLQGKIGGDS GLSSRGRKFA NALSKFVEEQ NLKDLKVWTS
310 320 330 340 350
QLKSTIQTAE ALQLPYEQWK ALNEIDAGVC EEMTYEEIKD TYPEEYALAE
360 370 380 390 400
ADKYYYRYPT GESYQDLVQR LEPVIMELER QENVLVICHQ AVCVCLLAYF
410 420 430 440 450
LDKSAEEMPY LKCPLHAVLK LTPIAYGCRV ESIYLNVESV STHRERSEDA
460
KKGPNPLMRS NSH
Sequence cautioni
The sequence AAB34145 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 463 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S77845 mRNA Translation: AAB34145.2 Different initiation. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S77845 mRNA Translation: AAB34145.2 Different initiation. |
3D structure databases
SMRi | Q28901 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000048148 |
Proteomic databases
PaxDbi | Q28901 |
PRIDEi | Q28901 |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
InParanoidi | Q28901 |
OMAi | DMLAWFR |
OrthoDBi | 392001at2759 |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 908 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F263_BOVIN | |
Accessioni | Q28901Primary (citable) accession number: Q28901 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families