UniProtKB - Q28719 (PTGR1_RABIT)
Prostaglandin reductase 1
PTGR1
Functioni
NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15-oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15-oxo-PGF2-alpha (By similarity).
Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (By similarity).
Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity).
Plays a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (By similarity).
May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity).
By similarityCatalytic activityi
- This reaction proceeds in the backwardBy similarity direction.
- EC:1.3.1.48By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 6-trans-leukotriene B4 + NADP+ = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + H+ + NADPHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + NADP+ = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + H+ + NADPHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:1.3.1.74By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 178 | NADPBy similarity | 1 | |
Binding sitei | 193 | NADPBy similarity | 1 | |
Binding sitei | 217 | NADPBy similarity | 1 | |
Binding sitei | 321 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 152 – 155 | NADPBy similarity | 4 | |
Nucleotide bindingi | 239 – 245 | NADPBy similarity | 7 | |
Nucleotide bindingi | 270 – 272 | NADPBy similarity | 3 |
GO - Molecular functioni
- 13-lipoxin reductase activity Source: UniProtKB
- 13-prostaglandin reductase activity Source: UniProtKB
- 15-oxoprostaglandin 13-oxidase activity Source: UniProtKB-EC
- 2-alkenal reductase (NADP+) activity Source: UniProtKB
- leukotriene B4 12-hydroxy dehydrogenase activity Source: UniProtKB
GO - Biological processi
- leukotriene B4 metabolic process Source: UniProtKB
- lipoxin A4 metabolic process Source: UniProtKB
- prostaglandin metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism, Prostaglandin metabolism |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Prostaglandin reductase 1Short name: PRG-1 Alternative name(s): 15-oxoprostaglandin 13-reductase (EC:1.3.1.48By similarity) ADRAB-F Dithiolethione-inducible gene 1 proteinBy similarity Short name: D3T-inducible gene 1 proteinBy similarity Short name: DIG-1By similarity Leukotriene B4 12-hydroxydehydrogenaseBy similarity NAD(P)H-dependent alkenal/one oxidoreductaseBy similarity (EC:1.3.1.74By similarity) |
Gene namesi | Name:PTGR1 Synonyms:LTB4DH |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218068 | 1 – 349 | Prostaglandin reductase 1Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 20 | PhosphoserineBy similarity | 1 | |
Modified residuei | 178 | N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity | 1 | |
Modified residuei | 178 | N6-acetyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, Hydroxylation, PhosphoproteinProteomic databases
PRIDEi | Q28719 |
Interactioni
Subunit structurei
Monomer or homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000004219 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1196, Eukaryota |
InParanoidi | Q28719 |
OrthoDBi | 884151at2759 |
Family and domain databases
CDDi | cd08294, leukotriene_B4_DH_like, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR041694, ADH_N_2 IPR011032, GroES-like_sf IPR045010, MDR_fam IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER IPR014190, PTGR1 |
PANTHERi | PTHR43205, PTHR43205, 1 hit |
Pfami | View protein in Pfam PF16884, ADH_N_2, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02825, B4_12hDH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVRAKNWTLK KHFHGHPTDS DFELKTVELP PLNNGEVLLE ALFLSVDPYM
60 70 80 90 100
RLGSKRLKEG DTMMGQQVAR VVESKNPAWP VGTLVLAHSG WASHSISDGQ
110 120 130 140 150
QLEKLLTEWP DTLPLSLALG TVGMPGITAY FGLLEICGAK SGDTVLVNAA
160 170 180 190 200
AGAVGAVVGQ IAKIKGCRVV GAAGSEEKVD YLKKIGFDFA FNYKTVKSLE
210 220 230 240 250
ETLKKAAPDG YDCYFDNVGG EFSNTVIRQM KKFGRVAICG AISMYNSTGQ
260 270 280 290 300
LPPGPSPESV LYQEIRMEGF IFNRWKGEVG QKALKELLTW VLEGKIQYRE
310 320 330 340
FVIEGFENMP AAFMRMLKGE NVGKARSESL KSGTCKPGDH PHDLIFPIT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34285 mRNA Translation: CAA84039.1 |
PIRi | S47093 |
RefSeqi | NP_001076171.1, NM_001082702.1 |
Genome annotation databases
GeneIDi | 100009440 |
KEGGi | ocu:100009440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34285 mRNA Translation: CAA84039.1 |
PIRi | S47093 |
RefSeqi | NP_001076171.1, NM_001082702.1 |
3D structure databases
SMRi | Q28719 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000004219 |
Proteomic databases
PRIDEi | Q28719 |
Genome annotation databases
GeneIDi | 100009440 |
KEGGi | ocu:100009440 |
Organism-specific databases
CTDi | 22949 |
Phylogenomic databases
eggNOGi | KOG1196, Eukaryota |
InParanoidi | Q28719 |
OrthoDBi | 884151at2759 |
Family and domain databases
CDDi | cd08294, leukotriene_B4_DH_like, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR041694, ADH_N_2 IPR011032, GroES-like_sf IPR045010, MDR_fam IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER IPR014190, PTGR1 |
PANTHERi | PTHR43205, PTHR43205, 1 hit |
Pfami | View protein in Pfam PF16884, ADH_N_2, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02825, B4_12hDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTGR1_RABIT | |
Accessioni | Q28719Primary (citable) accession number: Q28719 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families