UniProtKB - Q28685 (DAG1_RABIT)
Dystroglycan 1
DAG1
Functioni
The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
By similarityExtracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells (By similarity).
Also acts as a receptor for laminin LAMA5 (By similarity).
By similarityTransmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity (By similarity).
By similarityGO - Molecular functioni
- actin binding Source: Ensembl
- alpha-actinin binding Source: Ensembl
- calcium ion binding Source: InterPro
- dystroglycan binding Source: Ensembl
- laminin-1 binding Source: UniProtKB
- SH2 domain binding Source: Ensembl
- structural constituent of muscle Source: Ensembl
- tubulin binding Source: Ensembl
- vinculin binding Source: Ensembl
- virus receptor activity Source: Ensembl
GO - Biological processi
- basement membrane organization Source: Ensembl
- branching involved in salivary gland morphogenesis Source: Ensembl
- commissural neuron axon guidance Source: Ensembl
- epithelial tube branching involved in lung morphogenesis Source: Ensembl
- intracellular signal transduction Source: CACAO
- membrane protein ectodomain proteolysis Source: Ensembl
- microtubule anchoring Source: Ensembl
- morphogenesis of an epithelial sheet Source: Ensembl
- myelination in peripheral nervous system Source: Ensembl
- negative regulation of cell migration Source: Ensembl
- negative regulation of MAPK cascade Source: Ensembl
- negative regulation of protein kinase B signaling Source: Ensembl
- nerve maturation Source: Ensembl
- positive regulation of basement membrane assembly involved in embryonic body morphogenesis Source: Ensembl
- regulation of epithelial to mesenchymal transition Source: Ensembl
- regulation of gastrulation Source: Ensembl
- regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: Ensembl
- regulation of synapse organization Source: Ensembl
- retrograde trans-synaptic signaling by trans-synaptic protein complex Source: Ensembl
Protein family/group databases
MEROPSi | S72.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Dystroglycan 1By similarityAlternative name(s): Dystroglycan1 Publication Dystrophin-associated glycoprotein 1By similarity Cleaved into the following 2 chains: |
Gene namesi | Name:DAG1By similarity |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular space By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein
- sarcolemma By similarity
- postsynaptic cell membrane By similarity
Cytoskeleton
Nucleus
Note: The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3 (By similarity). Localizes to neuromuscular junctions (NMJs) in the presence of ANK2 (By similarity). Colocalizes with ERM proteins in Schwann-cell microvilli (By similarity). In peripheral nerves, localizes to the Schwann cell membrane.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: UniProtKB-SubCell
Plasma Membrane
- dystroglycan complex Source: Ensembl
- dystrophin-associated glycoprotein complex Source: UniProtKB
- external side of plasma membrane Source: Ensembl
- plasma membrane Source: Reactome
- postsynaptic membrane Source: UniProtKB-SubCell
- sarcolemma Source: UniProtKB-SubCell
Other locations
- basement membrane Source: Ensembl
- cell-cell junction Source: Ensembl
- contractile ring Source: Ensembl
- cytoplasm Source: UniProtKB-KW
- filopodium Source: Ensembl
- focal adhesion Source: Ensembl
- GABA-ergic synapse Source: Ensembl
- integral component of membrane Source: UniProtKB-KW
- lamellipodium Source: Ensembl
- membrane raft Source: Ensembl
- node of Ranvier Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 654 – 749 | ExtracellularAdd BLAST | 96 | |
Transmembranei | 750 – 775 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 776 – 895 | CytoplasmicSequence analysisAdd BLAST | 120 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Secreted, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | Sequence analysisAdd BLAST | 29 | |
ChainiPRO_0000021069 | 30 – 653 | Alpha-dystroglycanAdd BLAST | 624 | |
ChainiPRO_0000021070 | 654 – 895 | Beta-dystroglycanAdd BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 141 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 182 ↔ 264 | By similarity | ||
Glycosylationi | 317 | O-linked (Man6P...) threonineBy similarity | 1 | |
Glycosylationi | 319 | O-linked (Man6P...) threonineBy similarity | 1 | |
Glycosylationi | 379 | O-linked (Man6P...) threonineBy similarity | 1 | |
Glycosylationi | 641 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 649 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 661 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 669 ↔ 713 | By similarity | ||
Modified residuei | 790 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 892 | Phosphotyrosine; by SRCBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 653 – 654 | Cleavage; by autolysisBy similarity | 2 | |
Sitei | 715 – 716 | Cleavage; by MMP9By similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PRIDEi | Q28685 |
PTM databases
GlyConnecti | 40, 4 O-Linked glycans |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSOCUG00000003439, Expressed in upper lobe of left lung and 18 other tissues |
Interactioni
Subunit structurei
Monomer. Heterodimer of alpha- and beta-dystroglycan subunits which are the central components of the dystrophin-glycoprotein complex. This complex then can form a dystrophin-associated glycoprotein complex (DGC) which is composed of three subcomplexes: a cytoplasmic complex comprised of DMD (or UTRN), DTNA and a number of syntrophins, such as SNTB1, SNTB2, SNTG1 and SNTG2, the transmembrane dystroglycan complex, and the sarcoglycan-sarcospan complex.
Interacts (via the N-terminal of alphaDAG1) with LARGE1; the interaction enhances laminin binding.
Interacts with SGCD.
Interacts with AGR2 and AGR3.
Interacts (betaDAG1) with DMD; the interaction is inhibited by phosphorylation on the PPXY motif.
Interacts (betaDAG1, via its PPXY motif) with UTRN (via its WWW and ZZ domains); the interaction is inhibited by phosphorylation on the PPXY motif.
Interacts (betaDAG1, via its phosphorylated PPXY motif) with the SH2 domain-containing proteins, FYN, CSK, NCK and SHC.
Interacts (betaDAG1) with CAV3 (via a central WW-like domain); the interaction disrupts the binding of DMD. BetaDAG1 directly interacts with ANK3, but not with ANK2; this interaction does not interfere with DMD-binding and is required for retention at costameres (By similarity).
Identified in a dystroglycan complex that contains at least PRX, DRP2, UTRN, DMD and DAG1 (By similarity).
Interacts with POMGNT1 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- actin binding Source: Ensembl
- alpha-actinin binding Source: Ensembl
- dystroglycan binding Source: Ensembl
- laminin-1 binding Source: UniProtKB
- SH2 domain binding Source: Ensembl
- tubulin binding Source: Ensembl
- vinculin binding Source: Ensembl
Protein-protein interaction databases
CORUMi | Q28685 |
DIPi | DIP-679N |
IntActi | Q28685, 2 interactors |
MINTi | Q28685 |
STRINGi | 9986.ENSOCUP00000002981 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 603 – 712 | Peptidase S72Add BLAST | 110 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 408 | Required for laminin recognitionAdd BLAST | 379 | |
Regioni | 49 – 71 | O-glycosylated at one siteBy similarityAdd BLAST | 23 | |
Regioni | 316 – 485 | Mucin-like domainBy similarityAdd BLAST | 170 | |
Regioni | 348 – 369 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 413 – 499 | DisorderedSequence analysisAdd BLAST | 87 | |
Regioni | 463 – 485 | O-glycosylated at seven sites with GalNAcBy similarityAdd BLAST | 23 | |
Regioni | 724 – 747 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 819 – 895 | Required for interaction with CAV3By similarityAdd BLAST | 77 | |
Regioni | 823 – 895 | DisorderedSequence analysisAdd BLAST | 73 | |
Regioni | 880 – 895 | Required for binding DMD and UTRNBy similarityAdd BLAST | 16 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 776 – 782 | Nuclear localization signalBy similarity | 7 | |
Motifi | 889 – 892 | PPXY motifBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 348 – 363 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 416 – 449 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 468 – 490 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 858 – 873 | Pro residuesSequence analysisAdd BLAST | 16 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3781, Eukaryota |
GeneTreei | ENSGT00390000008429 |
HOGENOMi | CLU_007629_2_0_1 |
InParanoidi | Q28685 |
OMAi | LHRMQSF |
OrthoDBi | 163609at2759 |
TreeFami | TF328370 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits 3.30.70.1040, 1 hit |
InterProi | View protein in InterPro IPR027468, Alpha-dystroglycan_domain_2 IPR041631, Alpha_DG1_N2 IPR006644, Cadg IPR015919, Cadherin-like_sf IPR008465, DAG1_C IPR013783, Ig-like_fold IPR030398, SEA_DG_dom |
Pfami | View protein in Pfam PF18424, a_DG1_N2, 1 hit PF05454, DAG1, 1 hit |
SMARTi | View protein in SMART SM00736, CADG, 2 hits |
SUPFAMi | SSF111006, SSF111006, 1 hit SSF49313, SSF49313, 2 hits |
PROSITEi | View protein in PROSITE PS51699, SEA_DG, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MRMSVGLSLL LPLWGRTFLL LLCVAVAQSH WPSEPSEAVR DWENQLEASM
60 70 80 90 100
HSVLSDLHEA LPTVVGIPDG TAVVGRSFRV TIPTDLIGSS GEVIKVSTAG
110 120 130 140 150
KEVLPSWLHW DPQSHTLEGL PLDTDKGVHY ISVSAAQLDA NGSHIPQTSS
160 170 180 190 200
VFSIEVYPED HSEPQSVRAA SPDLGEAAAS ACAAEEPVTV LTVILDADLT
210 220 230 240 250
KMTPKQRIDL LHRMQSFSEV ELHNMKLVPV VNNRLFDMSA FMAGPGNAKK
260 270 280 290 300
VVENGALLSW KLGCSLNQNS VPDIRGVEAP AREGTMSAQL GYPVVGWHIA
310 320 330 340 350
NKKPPLPKRI RRQIHATPTP VTAIGPPTTA IQEPPSRIVP TPTSPAIAPP
360 370 380 390 400
TETMAPPVRD PVPGKPTVTT RTRGAIIQTP TLGPIQPTRV SDAGTVVSGQ
410 420 430 440 450
IRATVTIPGY VEPTAVATPP TTTTKKPRVS TPKPATPSTD SSATTTRRPT
460 470 480 490 500
KKPRTPRPVP RVTTKAPITR LETASPPTRI RTTTSGVPRG GEPNQRPELK
510 520 530 540 550
NHIDRVDAWV GTYFEVKIPS DTFYDKEDTT TDKLKLTLKL REQQLVGEKS
560 570 580 590 600
WVQFNSNSQL MYGLPDSSHV GKHEYFMHAT DKGGLSAVDA FEIHVHKRPQ
610 620 630 640 650
GDKAPARFKA KFVGDPAPVV NDIHKKIALV KKLAFAFGDR NCSTVTLQNI
660 670 680 690 700
TRGSIVVEWT NNTLPLEPCP KEQITGLSRR IAEDNGQPRP AFTNALEPDF
710 720 730 740 750
KATSIAITGS GSCRHLQFIP VAPPGIPSSV TPPTEVPDRD PEKSSEDDVY
760 770 780 790 800
LHTVIPAVVV AAILLIAGII AMICYRKKRK GKLTLEDQAT FIKKGVPIIF
810 820 830 840 850
ADELDDSKPP PSSSMPLILQ EEKAPLPPPE YPSQSVPETT PLNQDTVGEY
860 870 880 890
TPLRDEDPNA PPYQPPPPFT APMEGKGSRP KNMTPYRSPP PYVPP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64393 mRNA Translation: CAA45732.1 |
PIRi | PN0662 PN0663 S20582 |
RefSeqi | NP_001095170.1, NM_001101700.1 XP_017199133.1, XM_017343644.1 XP_017199134.1, XM_017343645.1 |
Genome annotation databases
Ensembli | ENSOCUT00000003437; ENSOCUP00000002981; ENSOCUG00000003439 |
GeneIDi | 100009278 |
KEGGi | ocu:100009278 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64393 mRNA Translation: CAA45732.1 |
PIRi | PN0662 PN0663 S20582 |
RefSeqi | NP_001095170.1, NM_001101700.1 XP_017199133.1, XM_017343644.1 XP_017199134.1, XM_017343645.1 |
3D structure databases
SMRi | Q28685 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q28685 |
DIPi | DIP-679N |
IntActi | Q28685, 2 interactors |
MINTi | Q28685 |
STRINGi | 9986.ENSOCUP00000002981 |
Protein family/group databases
MEROPSi | S72.001 |
PTM databases
GlyConnecti | 40, 4 O-Linked glycans |
Proteomic databases
PRIDEi | Q28685 |
Genome annotation databases
Ensembli | ENSOCUT00000003437; ENSOCUP00000002981; ENSOCUG00000003439 |
GeneIDi | 100009278 |
KEGGi | ocu:100009278 |
Organism-specific databases
CTDi | 1605 |
Phylogenomic databases
eggNOGi | KOG3781, Eukaryota |
GeneTreei | ENSGT00390000008429 |
HOGENOMi | CLU_007629_2_0_1 |
InParanoidi | Q28685 |
OMAi | LHRMQSF |
OrthoDBi | 163609at2759 |
TreeFami | TF328370 |
Gene expression databases
Bgeei | ENSOCUG00000003439, Expressed in upper lobe of left lung and 18 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits 3.30.70.1040, 1 hit |
InterProi | View protein in InterPro IPR027468, Alpha-dystroglycan_domain_2 IPR041631, Alpha_DG1_N2 IPR006644, Cadg IPR015919, Cadherin-like_sf IPR008465, DAG1_C IPR013783, Ig-like_fold IPR030398, SEA_DG_dom |
Pfami | View protein in Pfam PF18424, a_DG1_N2, 1 hit PF05454, DAG1, 1 hit |
SMARTi | View protein in SMART SM00736, CADG, 2 hits |
SUPFAMi | SSF111006, SSF111006, 1 hit SSF49313, SSF49313, 2 hits |
PROSITEi | View protein in PROSITE PS51699, SEA_DG, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DAG1_RABIT | |
Accessioni | Q28685Primary (citable) accession number: Q28685 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |