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Protein

Aggrecan core protein

Gene

ACAN

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2184Calcium 1By similarity1
Metal bindingi2188Calcium 1By similarity1
Metal bindingi2188Calcium 3By similarity1
Metal bindingi2208Calcium 2By similarity1
Metal bindingi2210Calcium 2By similarity1
Metal bindingi2211Calcium 1By similarity1
Metal bindingi2217Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi2217Calcium 2By similarity1
Metal bindingi2218Calcium 1By similarity1
Metal bindingi2218Calcium 3By similarity1
Metal bindingi2231Calcium 2By similarity1
Metal bindingi2232Calcium 2By similarity1
Metal bindingi2232Calcium 2; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aggrecan core protein
Alternative name(s):
Cartilage-specific proteoglycan core protein
Short name:
CSPCP
Gene namesi
Name:ACAN
Synonyms:AGC1
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001750317 – 2333Aggrecan core proteinAdd BLAST2317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 133By similarity
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi175 ↔ 246By similarity
Disulfide bondi199 ↔ 220By similarity
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi273 ↔ 348By similarity
Disulfide bondi297 ↔ 318By similarity
Glycosylationi333N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi371O-linked (Xyl...) (keratan sulfate) threonineBy similarity1
Glycosylationi376O-linked (Xyl...) (keratan sulfate) threonineBy similarity1
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi444N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi518 ↔ 589By similarity
Disulfide bondi542 ↔ 563By similarity
Disulfide bondi616 ↔ 691By similarity
Glycosylationi620N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi640 ↔ 661By similarity
Glycosylationi676N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi747N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi2085 ↔ 2096By similarity
Disulfide bondi2090 ↔ 2105By similarity
Disulfide bondi2107 ↔ 2116By similarity
Disulfide bondi2123 ↔ 2134By similarity
Disulfide bondi2151 ↔ 2243By similarity
Disulfide bondi2219 ↔ 2235By similarity
Disulfide bondi2250 ↔ 2293By similarity
Disulfide bondi2279 ↔ 2306By similarity

Post-translational modificationi

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiQ28343
PRIDEiQ28343

Interactioni

Subunit structurei

Interacts with FBLN1 and COMP.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000016949

Structurei

3D structure databases

ProteinModelPortaliQ28343
SMRiQ28343
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 147Ig-like V-typeAdd BLAST114
Domaini153 – 248Link 1PROSITE-ProRule annotationAdd BLAST96
Domaini253 – 350Link 2PROSITE-ProRule annotationAdd BLAST98
Domaini496 – 591Link 3PROSITE-ProRule annotationAdd BLAST96
Domaini597 – 693Link 4PROSITE-ProRule annotationAdd BLAST97
Domaini2081 – 2117EGF-like; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini2130 – 2245C-type lectinPROSITE-ProRule annotationAdd BLAST116
Domaini2248 – 2308SushiPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 140G1-AAdd BLAST93
Regioni152 – 247G1-BAdd BLAST96
Regioni253 – 349G1-B'Add BLAST97
Regioni495 – 589G2-BAdd BLAST95
Regioni596 – 691G2-B'Add BLAST96
Regioni694 – 816KSAdd BLAST123
Regioni819 – 1394CS-1Add BLAST576
Regioni1395 – 2079CS-2Add BLAST685
Regioni2080 – 2333G3Add BLAST254

Domaini

Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IJP2 Eukaryota
ENOG410XRES LUCA
HOGENOMiHOG000168421
HOVERGENiHBG007982
InParanoidiQ28343

Family and domain databases

CDDicd00033 CCP, 1 hit
cd03588 CLECT_CSPGs, 1 hit
Gene3Di2.60.40.10, 1 hit
3.10.100.10, 5 hits
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033987 CSPG_CTLD
IPR016187 CTDL_fold
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR000538 Link_dom
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00059 Lectin_C, 1 hit
PF00084 Sushi, 1 hit
PF07686 V-set, 1 hit
PF00193 Xlink, 4 hits
PRINTSiPR01265 LINKMODULE
SMARTiView protein in SMART
SM00032 CCP, 1 hit
SM00034 CLECT, 1 hit
SM00181 EGF, 1 hit
SM00179 EGF_CA, 1 hit
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SM00445 LINK, 4 hits
SUPFAMiSSF48726 SSF48726, 1 hit
SSF56436 SSF56436, 5 hits
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit
PS01241 LINK_1, 4 hits
PS50963 LINK_2, 4 hits
PS50923 SUSHI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28343-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTLLWVFVT LRVITAASSE ETSDHDNSLS VSIPEPSPMR VLLGSSLTIP
60 70 80 90 100
CYFIDPMHPV TTAPSTAPLA PRIKWSRITK EKEVVLLVAT EGQVRINSAY
110 120 130 140 150
QDKVSLPNYP AIPSDATLEI QNLRSNDSGI YRCEVMHGIE DSEATLEVVV
160 170 180 190 200
KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD
210 220 230 240 250
AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE
260 270 280 290 300
MEGEVLYATS PEKFTFQEAA NECRRLGARL ATTGQLYLAW QGGMDMCSAG
310 320 330 340 350
WLADRSVRYP ISKARPNCGG NLLGVRTVYL HANQTGYPDP SSRYDAICYT
360 370 380 390 400
GEDFVDIPEN FFGVGGEEDI TIQTVTWPDV ELPLPRNITE GEARGNVILT
410 420 430 440 450
VKPIFDLSPT APEPEEPFTF VPEPEKPFTF ATDVGVTAFP EAENRTGEAT
460 470 480 490 500
RPWGVPEEST PGPAFTAFTS EDHVVQVTAV PGAAEVPGQP RLPGGVVFHY
510 520 530 540 550
RPGSARYSLT FEEAQQACLR TGAVIASPEQ LQAAYEAGYE QCDAGWLQDQ
560 570 580 590 600
TVRYPIVSPR TPCVGDKDSS PGVRTYGVRP PSETYDVYCY VDKLEGEVFF
610 620 630 640 650
ITRLEQFTFQ EALAFCESHN ATLASTGQLY AAWRQGLDKC YAGWLSDGSL
660 670 680 690 700
RYPIVTPRPS CGGDKPGVRT VYLYPNQTGL PDPLSRHHVF CFRGVSGVPS
710 720 730 740 750
PGEEEGGTPT PSVVEDWIPT QVGPVVPSVP MGEETTAILD FTIEPENQTE
760 770 780 790 800
WEPAYSPAGT SPLPGIPPTW PPTSTATEES TEGPSGTEVP SVSEEPSPSE
810 820 830 840 850
EPFPWEELST LSPPGPSGTE LPGSGEASGV PEVSGDFTGS GEVSGHPDSS
860 870 880 890 900
GQLSGESASG LPSEDLDSSG LTSAVGSGLA SGDEDRITLS SIPKVEGEGL
910 920 930 940 950
ETSASGVEDL SGLPSGREGL ETSTSGVGDL SGLPSGEGLE VSASGVEDLS
960 970 980 990 1000
GLPSGEGPET STSGVGDLSR LPSGEGPEVS ASGVGDLSGL PSGREGLETS
1010 1020 1030 1040 1050
TSGVEDLSGL PSGEGPEAST SGVGDLSRLP SGEGPEVSAS GVEDLSGLPS
1060 1070 1080 1090 1100
GEGLEASASG VGDLSGLPSG EGPEASASGV GDLSRLPSGE GPEVSASGVE
1110 1120 1130 1140 1150
DLSGLSSGES PEASASGVGD LSGLPSGREG LETSASGVGD LSGLPSGEGQ
1160 1170 1180 1190 1200
EASASGVEDL SRLPSGEGPE ASASGVGELS GLPSGREGLE TSASGVGDLS
1210 1220 1230 1240 1250
GLPSGEGPEA FASGVEDLSI LPSGEGPEAS ASGVGDLSGL PSGREGLETS
1260 1270 1280 1290 1300
TSGVGDLSGL PSGREGLETS TSGVGDLSGL PSGEGPEASA SGIGDISGLP
1310 1320 1330 1340 1350
SGREGLETSS SGVEDHPETS ASGVEDLSGL PSGVEGHPET SASGVEDLSD
1360 1370 1380 1390 1400
LSSGGEGLET SASGAEDLSG FPSGKEDLIG SASGALDFGR IPSGTLGSGQ
1410 1420 1430 1440 1450
APEASSLPSG FSGEYSGVDF GSGPISGLPD FSGLPSGFPT ISLVDTTLVE
1460 1470 1480 1490 1500
VITTTSASEL EGRGTIGISG AGETSGLPVS ELDISGAVSG LPSGAELSGQ
1510 1520 1530 1540 1550
ASGSPDMSGE TSGFFGVSGQ PSGFPDISGG TSGLFEVSGQ PSGFSGETSG
1560 1570 1580 1590 1600
VTELSGLYSG QPDVSGEASG VPSGSGQPFG MTDLSGETSG VPDISGQPSG
1610 1620 1630 1640 1650
LPEFSGTTSG IPDLVSSTMS GSGESSGITF VDTSLVEVTP TTFKEKKRLG
1660 1670 1680 1690 1700
SVELSGLPSG EVDLSGASGT MDISGQSSGA TDSSGLTSHL PKFSGLPSGA
1710 1720 1730 1740 1750
AEVSGESSGA EVGSSLPSGT YEGSGNFHPA FPTVFLVDRT LVESVTQAPT
1760 1770 1780 1790 1800
AQEAGEGPSG ILELSGAHSG APDVSGDHSG SLDLSGMQSG LVEPSGEPSS
1810 1820 1830 1840 1850
TPYFSGDFSG TMDVTGEPST AMSASGEASG LLEVTLITSE FVEGVTEPTV
1860 1870 1880 1890 1900
SQELAQRPPV THTPQLFESS GEASASGEIS GATPAFPGSG LEASSVPESS
1910 1920 1930 1940 1950
SETSDFPERA VGVSAAPEAS GGASGAPDVS EATSTFPEAD VEGASGLGVS
1960 1970 1980 1990 2000
GGTSAFPEAP REGSATPEVQ EEPTTSYDVG REALGWPSAT PTASGDRIEV
2010 2020 2030 2040 2050
SGDLSGHTSG LDVVISTSVP ESEWIQQTQR PAEAHLEIEA SSPLHSGEET
2060 2070 2080 2090 2100
QTAETATSPT DDASIPTSPS GTDESAPAIP DIDECLSSPC LNGATCVDAI
2110 2120 2130 2140 2150
DSFTCLCLPS YRGDLCEIDQ ELCEEGWTKF QGHCYRYFPD RESWVDAESR
2160 2170 2180 2190 2200
CRAQQSHLSS IVTPEEQEFV NNNAQDYQWI GLNDRTIEGD FRWSDGHSLQ
2210 2220 2230 2240 2250
FENWRPNQPD NFFVSGEDCV VMIWHEKGEW NDVPCNYYLP FTCKKGTVAC
2260 2270 2280 2290 2300
GDPPVVEHAR TFGQKKDRYE INSLVRYQCT EGFVQRHVPT IRCQPSGHWE
2310 2320 2330
KPRITCTDPS TYKRRLQKRS SRAPRRSRPS TAH
Length:2,333
Mass (Da):240,576
Last modified:January 11, 2001 - v2
Checksum:i8B9ED78F3508B596
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65989 mRNA Translation: AAB06238.2
S74662 mRNA Translation: AAC60527.1
L07054 mRNA No translation available.
PIRiI46998
UniGeneiCfa.3605

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65989 mRNA Translation: AAB06238.2
S74662 mRNA Translation: AAC60527.1
L07054 mRNA No translation available.
PIRiI46998
UniGeneiCfa.3605

3D structure databases

ProteinModelPortaliQ28343
SMRiQ28343
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000016949

Proteomic databases

PaxDbiQ28343
PRIDEiQ28343

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IJP2 Eukaryota
ENOG410XRES LUCA
HOGENOMiHOG000168421
HOVERGENiHBG007982
InParanoidiQ28343

Family and domain databases

CDDicd00033 CCP, 1 hit
cd03588 CLECT_CSPGs, 1 hit
Gene3Di2.60.40.10, 1 hit
3.10.100.10, 5 hits
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033987 CSPG_CTLD
IPR016187 CTDL_fold
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR000538 Link_dom
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00059 Lectin_C, 1 hit
PF00084 Sushi, 1 hit
PF07686 V-set, 1 hit
PF00193 Xlink, 4 hits
PRINTSiPR01265 LINKMODULE
SMARTiView protein in SMART
SM00032 CCP, 1 hit
SM00034 CLECT, 1 hit
SM00181 EGF, 1 hit
SM00179 EGF_CA, 1 hit
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SM00445 LINK, 4 hits
SUPFAMiSSF48726 SSF48726, 1 hit
SSF56436 SSF56436, 5 hits
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit
PS01241 LINK_1, 4 hits
PS50963 LINK_2, 4 hits
PS50923 SUSHI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGCA_CANLF
AccessioniPrimary (citable) accession number: Q28343
Secondary accession number(s): Q28310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: June 20, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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