UniProtKB - Q28300 (GNAT1_CANLF)
Protein
Guanine nucleotide-binding protein G(t) subunit alpha-1
Gene
GNAT1
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | MagnesiumBy similarity | 1 | |
Binding sitei | 146 | GTPBy similarity | 1 | |
Metal bindingi | 177 | MagnesiumBy similarity | 1 | |
Binding sitei | 199 | GTP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 322 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | GTPBy similarity | 8 | |
Nucleotide bindingi | 171 – 177 | GTPBy similarity | 7 | |
Nucleotide bindingi | 265 – 268 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell population proliferation Source: Ensembl
- cellular response to electrical stimulus Source: Ensembl
- detection of light stimulus involved in visual perception Source: Ensembl
- eye photoreceptor cell development Source: Ensembl
- G protein-coupled adenosine receptor signaling pathway Source: GO_Central
- phototransduction, visible light Source: Ensembl
- response to light stimulus Source: AgBase
- retina development in camera-type eye Source: Ensembl
- sensory perception of umami taste Source: Ensembl
Keywordsi
Molecular function | Transducer |
Biological process | Sensory transduction, Vision |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-CFA-112043, PLC beta mediated events R-CFA-202040, G-protein activation R-CFA-2485179, Activation of the phototransduction cascade R-CFA-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-CFA-418594, G alpha (i) signalling events R-CFA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(t) subunit alpha-1Alternative name(s): Transducin alpha-1 chain1 Publication |
Gene namesi | Name:GNAT1 |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:41307, GNAT1 |
Subcellular locationi
Other locations
- photoreceptor outer segment By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
- Photoreceptor inner segment By similarity
Note: Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.By similarity
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
Other locations
- membrane Source: AgBase
- photoreceptor connecting cilium Source: Ensembl
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: UniProtKB
Keywords - Cellular componenti
Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000203736 | 2 – 350 | Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 142 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
PaxDbi | Q28300 |
Expressioni
Tissue specificityi
Rod.
Gene expression databases
Bgeei | ENSCAFG00000010764, Expressed in liver and 23 other tissues |
Interactioni
Subunit structurei
Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Interacts with RHO.
Interacts with RGS9 and PDE6G (By similarity).
Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).
By similarityGO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000015846 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 350 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 31 – 44 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 169 – 177 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 192 – 201 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 261 – 268 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 320 – 325 | G5 motifPROSITE-ProRule annotation | 6 | |
Regioni | 340 – 350 | Interaction with RHOBy similarityAdd BLAST | 11 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000160395 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | Q28300 |
OMAi | ECLEFIV |
OrthoDBi | 754573at2759 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q28300-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFERAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69597 mRNA Translation: CAA93443.1 U65376 mRNA Translation: AAB39560.1 AJ506760 Genomic DNA Translation: CAD45009.1 |
RefSeqi | NP_001003068.1, NM_001003068.3 XP_013977200.1, XM_014121725.1 |
Genome annotation databases
Ensembli | ENSCAFT00000017125; ENSCAFP00000015846; ENSCAFG00000010764 ENSCAFT00030022907; ENSCAFP00030019983; ENSCAFG00030012361 |
GeneIDi | 403613 |
KEGGi | cfa:403613 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69597 mRNA Translation: CAA93443.1 U65376 mRNA Translation: AAB39560.1 AJ506760 Genomic DNA Translation: CAD45009.1 |
RefSeqi | NP_001003068.1, NM_001003068.3 XP_013977200.1, XM_014121725.1 |
3D structure databases
SMRi | Q28300 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000015846 |
Proteomic databases
PaxDbi | Q28300 |
Genome annotation databases
Ensembli | ENSCAFT00000017125; ENSCAFP00000015846; ENSCAFG00000010764 ENSCAFT00030022907; ENSCAFP00030019983; ENSCAFG00030012361 |
GeneIDi | 403613 |
KEGGi | cfa:403613 |
Organism-specific databases
CTDi | 2779 |
VGNCi | VGNC:41307, GNAT1 |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000160395 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | Q28300 |
OMAi | ECLEFIV |
OrthoDBi | 754573at2759 |
TreeFami | TF300673 |
Enzyme and pathway databases
Reactomei | R-CFA-112043, PLC beta mediated events R-CFA-202040, G-protein activation R-CFA-2485179, Activation of the phototransduction cascade R-CFA-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-CFA-418594, G alpha (i) signalling events R-CFA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Gene expression databases
Bgeei | ENSCAFG00000010764, Expressed in liver and 23 other tissues |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAT1_CANLF | |
Accessioni | Q28300Primary (citable) accession number: Q28300 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families