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Protein

Glial fibrillary acidic protein

Gene

GFAP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Glial fibrillary acidic protein
Short name:
GFAP
Gene namesi
Name:GFAP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Organism-specific databases

VGNCiVGNC:29323 GFAP

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638041 – 428Glial fibrillary acidic proteinAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphothreonine; by AURKB and ROCK1By similarity1
Modified residuei12Omega-N-methylarginineBy similarity1
Modified residuei13Phosphoserine; by AURKB and ROCK1By similarity1
Modified residuei26CitrullineBy similarity1
Modified residuei32CitrullineBy similarity1
Modified residuei34Phosphoserine; by AURKB and ROCK1By similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei146PhosphothreonineBy similarity1
Modified residuei266CitrullineBy similarity1
Modified residuei319PhosphoserineBy similarity1
Modified residuei379PhosphothreonineBy similarity1
Modified residuei381PhosphoserineBy similarity1
Modified residuei402CitrullineBy similarity1
Modified residuei412CitrullineBy similarity1

Post-translational modificationi

Phosphorylated by PKN1.By similarity

Keywords - PTMi

Citrullination, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ28115
PeptideAtlasiQ28115
PRIDEiQ28115

PTM databases

iPTMnetiQ28115

Expressioni

Gene expression databases

BgeeiENSBTAG00000013534 Expressed in 7 organ(s), highest expression level in prefrontal cortex

Interactioni

Subunit structurei

Interacts with SYNM.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PASKQ96RG22EBI-907866,EBI-1042651From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ28115, 2 interactors
MINTiQ28115
STRINGi9913.ENSBTAP00000017997

Structurei

3D structure databases

ProteinModelPortaliQ28115
SMRiQ28115
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 373IF rodPROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 68HeadAdd BLAST68
Regioni69 – 100Coil 1AAdd BLAST32
Regioni101 – 111Linker 1Add BLAST11
Regioni112 – 210Coil 1BAdd BLAST99
Regioni211 – 226Linker 12Add BLAST16
Regioni227 – 248Coil 2AAdd BLAST22
Regioni249 – 252Linker 24
Regioni253 – 373Coil 2BAdd BLAST121
Regioni374 – 428TailAdd BLAST55

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJXF Eukaryota
ENOG410Y9QE LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiQ28115
KOiK05640
OMAiAQQVHVD
OrthoDBiEOG091G12MK
TreeFamiTF330122

Family and domain databases

InterProiView protein in InterPro
IPR027701 GFAP
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF41 PTHR23239:SF41, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q28115-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERRRVTSAT RRSYVSSSEM VVGGRRLGPG TRLSLARMPP PLPARVDFSL
60 70 80 90 100
AGALNSGFKE TRASERAEMM ELNDRFASYI EKVRFLEQQN KALAAELNQL
110 120 130 140 150
RAKEPTKLAD VYQAELRELR LRLDQLTANS ARLEVERDNL AQDLGTLRQK
160 170 180 190 200
LQDETNQRLE AENNLAAYRQ EADEATLARL DLERKIESLE EEIRFLRKIH
210 220 230 240 250
EEEVRELQEQ LAQQQVHVEM DVAKPDLTAA LREIRTQYEA VASSNMHEAE
260 270 280 290 300
EWYRSKFADL NDAAARNAEL LRQAKHEAND YRRQLQALTC DLESLRGTNE
310 320 330 340 350
SLERQMREQE ERHAREAASY QEALARLEEE GQSLKDEMAR HLQEYQDLLN
360 370 380 390 400
VKLALDIEIA TYRKLLEGEE NRITIPVQTF SNLQIRETSL DTKSVSEGHL
410 420
KRNIVVKTVE MRDGEVIKES KQEHKDVM
Length:428
Mass (Da):49,512
Last modified:April 3, 2007 - v2
Checksum:i8DCCDF3E848BAD05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265A → R in CAA69422 (PubMed:10571109).Curated1
Sequence conflicti265A → R in AAO18221 (Ref. 3) Curated1
Sequence conflicti271L → V in CAA69422 (PubMed:10571109).Curated1
Sequence conflicti271L → V in AAO18221 (Ref. 3) Curated1
Sequence conflicti311 – 312ER → DA in CAA69422 (PubMed:10571109).Curated2
Sequence conflicti311 – 312ER → DA in AAO18221 (Ref. 3) Curated2
Sequence conflicti372R → Q in AAO18221 (Ref. 3) Curated1
Sequence conflicti421K → P in CAA69422 (PubMed:10571109).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA Translation: CAA69422.1
BC119892 mRNA Translation: AAI19893.1
AY174179 Genomic DNA Translation: AAO18221.1 Different termination.
L19867 Genomic DNA Translation: AAA51413.1
PIRiPC2280
RefSeqiNP_776490.2, NM_174065.2
UniGeneiBt.21127

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534
GeneIDi281189
KEGGibta:281189

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA Translation: CAA69422.1
BC119892 mRNA Translation: AAI19893.1
AY174179 Genomic DNA Translation: AAO18221.1 Different termination.
L19867 Genomic DNA Translation: AAA51413.1
PIRiPC2280
RefSeqiNP_776490.2, NM_174065.2
UniGeneiBt.21127

3D structure databases

ProteinModelPortaliQ28115
SMRiQ28115
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ28115, 2 interactors
MINTiQ28115
STRINGi9913.ENSBTAP00000017997

PTM databases

iPTMnetiQ28115

Proteomic databases

PaxDbiQ28115
PeptideAtlasiQ28115
PRIDEiQ28115

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534
GeneIDi281189
KEGGibta:281189

Organism-specific databases

CTDi2670
VGNCiVGNC:29323 GFAP

Phylogenomic databases

eggNOGiENOG410IJXF Eukaryota
ENOG410Y9QE LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiQ28115
KOiK05640
OMAiAQQVHVD
OrthoDBiEOG091G12MK
TreeFamiTF330122

Gene expression databases

BgeeiENSBTAG00000013534 Expressed in 7 organ(s), highest expression level in prefrontal cortex

Family and domain databases

InterProiView protein in InterPro
IPR027701 GFAP
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF41 PTHR23239:SF41, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGFAP_BOVIN
AccessioniPrimary (citable) accession number: Q28115
Secondary accession number(s): Q0P5L4, Q866S9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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