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Protein

Aspartyl/asparaginyl beta-hydroxylase

Gene

ASPH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.1 Publication

Catalytic activityi

Peptide L-aspartate + 2-oxoglutarate + O2 = peptide 3-hydroxy-L-aspartate + succinate + CO2.2 Publications

Cofactori

Fe cation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6212-oxoglutarateBy similarity1
Binding sitei6642-oxoglutarateBy similarity1
Metal bindingi675IronBy similarity1
Metal bindingi721IronBy similarity1
Binding sitei7312-oxoglutarateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi108 – 120By similarityAdd BLAST13

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • peptide-aspartate beta-dioxygenase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandCalcium, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-2672351 Stimuli-sensing channels
R-BTA-5578775 Ion homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/asparaginyl beta-hydroxylase (EC:1.14.11.161 Publication)
Alternative name(s):
Aspartate beta-hydroxylase
Short name:
ASP beta-hydroxylase
Peptide-aspartate beta-dioxygenase
Gene namesi
Name:ASPH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Organism-specific databases

VGNCiVGNC:26219 ASPH

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 56CytoplasmicSequence analysisAdd BLAST56
Transmembranei57 – 77Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini78 – 754LumenalSequence analysisAdd BLAST677

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000647051 – 754Aspartyl/asparaginyl beta-hydroxylaseAdd BLAST754

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi637 ↔ 644By similarity
Glycosylationi702N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Might be processed to the 56 kDa (AA 289-754) or 52 kDa (AA 311-754) forms in the lumen of the endoplasmic reticulum.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ28056
PeptideAtlasiQ28056
PRIDEiQ28056

Expressioni

Gene expression databases

BgeeiENSBTAG00000026283
ExpressionAtlasiQ28056 baseline and differential

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021267

Structurei

3D structure databases

ProteinModelPortaliQ28056
SMRiQ28056
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati337 – 370TPR 1Add BLAST34
Repeati378 – 411TPR 2Add BLAST34
Repeati450 – 483TPR 3Add BLAST34
Repeati485 – 517TPR 4Add BLAST33
Repeati521 – 553TPR 5Add BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni684 – 6862-oxoglutarate bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 12Poly-Gly4
Compositional biasi14 – 21Poly-Ser8
Compositional biasi311 – 352Lys-richAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal-anchor, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3696 Eukaryota
COG3555 LUCA
GeneTreeiENSGT00530000063281
HOGENOMiHOG000231625
HOVERGENiHBG004290
InParanoidiQ28056
KOiK00476
OMAiRNENACK
OrthoDBiEOG091G0H5R
TreeFamiTF312799

Family and domain databases

Gene3Di1.25.40.10, 2 hits
2.60.120.330, 1 hit
InterProiView protein in InterPro
IPR007943 Asp-B-hydro/Triadin_dom
IPR007803 Asp/Arg/Pro-Hydrxlase
IPR039038 ASPH
IPR027443 IPNS-like
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR12366 PTHR12366, 1 hit
PfamiView protein in Pfam
PF05279 Asp-B-Hydro_N, 1 hit
PF05118 Asp_Arg_Hydrox, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 2 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 2 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q28056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRKNAKGG GGNSSSSSSG SPTGCTSGGS SSPGARRETK QGGLKNGRKG
60 70 80 90 100
GLSGSSFFTW FMVIALLGVW TSVAVVWFDL VDYEEVLAKA KDFRYNLSEV
110 120 130 140 150
LQGKLGIYDA DGDGDFDVDD AKVLLGLKEK PAPKPTVPPE EADMYPWLED
160 170 180 190 200
QVLESPGRQN IEDEVYEQVQ SLDETVYSEP GENLPQEPEG PAEELQPDDH
210 220 230 240 250
VFVGSDADDR YEPMGTGAVH EETEDSYHIE ETASPAYSQD MEDMMYEQEN
260 270 280 290 300
PDSSEPVVVD DAERTYQETD DVTYRDYDEQ DHAVDNSNTI LEEPHMPPAE
310 320 330 340 350
EQQEVPPETN KKADEPGKKG KVKKKKPKLL NKFDKTIKAE LDAAEKLRKR
360 370 380 390 400
GKIEEAVNAF EELVRKYPQS PGARYGKAQC EDDLAEKRRS NEILRRAIET
410 420 430 440 450
YQEAASLPDA PTDLVKLSLK RRSDRQQFLG HMRGSLLTLQ KLVQLFPDDT
460 470 480 490 500
ALKNDLGVGY LLIGDNDSAK KVYEEVLSVT PNDGFAKVHY GFILKAQNKI
510 520 530 540 550
AESIPYLKEG IESGDPGTDD GRFYFHLGDA MQRVGNKEAY RWYELGHQRG
560 570 580 590 600
HFASVWQRSL YNVQGLKAQP WWTPKETGYT ELVKSLERNW KLIRDEGLAA
610 620 630 640 650
MDRTHGLFLP EDENLREKGD WSQFTLWQQG RKNENACKGA PKTCSLLDKF
660 670 680 690 700
PETTGCRRGQ IKYSIMHPGT HVWPHTGPTN CRLRMHLGLV IPKEGCKIRC
710 720 730 740 750
ANETRTWEEG KVLIFDDSFE HEVWQDAASF RLIFIVDVWH PELTPHQRRS

LPAI
Length:754
Mass (Da):84,999
Last modified:November 1, 1997 - v1
Checksum:i369593A1F0B558C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91213 mRNA Translation: AAA03563.1
PIRiA42969 BABOH
RefSeqiNP_777182.1, NM_174757.2
UniGeneiBt.46037

Genome annotation databases

EnsembliENSBTAT00000021267; ENSBTAP00000021267; ENSBTAG00000026283
GeneIDi286771
KEGGibta:286771

Similar proteinsi

Entry informationi

Entry nameiASPH_BOVIN
AccessioniPrimary (citable) accession number: Q28056
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 18, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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