UniProtKB - Q27ID4 (TERT_BOVIN)
Telomerase reverse transcriptase
TERT
Functioni
Catalytic activityi
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)PROSITE-ProRule annotationEC:2.7.7.49PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 169 | Required for optimal binding of telomeric ssDNA and incorporation of nucleotides at the second position of the templateBy similarity | 1 | |
Metal bindingi | 705 | Magnesium; catalyticPROSITE-ProRule annotation | 1 | |
Sitei | 860 | Required for nucleotide incorporation and primer extension rateBy similarity | 1 | |
Metal bindingi | 861 | Magnesium; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 862 | Magnesium; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- telomerase activity Source: UniProtKB
- telomerase RNA reverse transcriptase activity Source: InterPro
GO - Biological processi
- telomere maintenance via telomerase Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Ribonucleoprotein, RNA-directed DNA polymerase, Transferase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Telomerase reverse transcriptase (EC:2.7.7.49)Alternative name(s): Telomerase catalytic subunit |
Gene namesi | Name:TERT |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- nucleolus By similarity
- nucleoplasm By similarity
- Nucleus
- PML body By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
Note: Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-700. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT (By similarity).By similarity
Nucleus
- nucleolus Source: UniProtKB
- PML body Source: UniProtKB-SubCell
- telomerase holoenzyme complex Source: UniProtKB
Other locations
- chromosome, telomeric region Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chromosome, Cytoplasm, Nucleus, TelomerePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245166 | 1 – 1125 | Telomerase reverse transcriptaseAdd BLAST | 1125 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 231 | Phosphoserine; by PKB/AKT1By similarity | 1 | |
Modified residuei | 450 | Phosphoserine; by DYRK2By similarity | 1 | |
Modified residuei | 700 | Phosphotyrosine; by SRC-type Tyr-kinasesBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q27ID4 |
PRIDEi | Q27ID4 |
Interactioni
Subunit structurei
Catalytic component of the telomerase holoenzyme complex composed of one molecule of TERT, one molecule of WRAP53/TCAB1, two molecules of H/ACA ribonucleoprotein complex subunits DKC1, NOP10, NHP2 and GAR1, and a telomerase RNA template component (TERC). The telomerase holoenzyme complex is associated with TEP1, SMG6/EST1A and POT1. The molecular chaperone HSP90/P23 complex is required for correct assembly and stabilization of the active telomerase.
Interacts directly with HSP90A and PTGES3.
Interacts with HSPA1A; the interaction occurs in the absence of TERC and dissociates once the complex has formed.
Interacts with RAN; the interaction promotes nuclear export of TERT.
Interacts with XPO1.
Interacts with PTPN11; the interaction retains TERT in the nucleus.
Interacts with NCL (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains); the interaction is important for nucleolar localization of TERT (By similarity).
Interacts with SMARCA4 (via the bromodomain); the interaction regulates Wnt-mediated signaling (By similarity).
Interacts with MCRS1 (isoform MCRS2); the interaction inhibits in vitro telomerase activity (By similarity).
Interacts with PIF1; the interaction has no effect on the elongation activity of TERT (By similarity).
Interacts with PML; the interaction recruits TERT to PML bodies and inhibits telomerase activity (By similarity).
Interacts with GNL3L (By similarity).
Interacts with isoform 1 and isoform 2 of NVL (By similarity).
Interacts with DHX36 (By similarity).
Interacts with ATF7 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016685 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 598 – 928 | Reverse transcriptasePROSITE-ProRule annotationAdd BLAST | 331 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 234 | RNA-interacting domain 1By similarityAdd BLAST | 234 | |
Regioni | 58 – 199 | GQ motifBy similarityAdd BLAST | 142 | |
Regioni | 137 – 141 | Required for regulating specificity for telomeric DNA and for processivity for primer elongationBy similarity | 5 | |
Regioni | 235 – 312 | LinkerBy similarityAdd BLAST | 78 | |
Regioni | 290 – 531 | Required for oligomerizationBy similarityAdd BLAST | 242 | |
Regioni | 313 – 543 | RNA-interacting domain 2By similarityAdd BLAST | 231 | |
Regioni | 364 – 514 | QFP motifBy similarityAdd BLAST | 151 | |
Regioni | 385 – 405 | CP motifBy similarityAdd BLAST | 21 | |
Regioni | 907 – 921 | Required for oligomerizationBy similarityAdd BLAST | 15 | |
Regioni | 923 – 927 | Primer grip sequenceBy similarity | 5 | |
Regioni | 929 – 1125 | CTEBy similarityAdd BLAST | 197 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 226 – 244 | Bipartite nuclear localization signalBy similarityAdd BLAST | 19 | |
Motifi | 316 – 321 | TFLY; involved in RNA bindingBy similarity | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 180 – 185 | Poly-Ala | 6 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1005, Eukaryota |
InParanoidi | Q27ID4 |
OrthoDBi | 1297956at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR000477, RT_dom IPR021891, Telomerase_RBD IPR003545, Telomerase_RT |
PANTHERi | PTHR12066, PTHR12066, 1 hit |
Pfami | View protein in Pfam PF00078, RVT_1, 1 hit PF12009, Telomerase_RBD, 1 hit |
PRINTSi | PR01365, TELOMERASERT |
SMARTi | View protein in SMART SM00975, Telomerase_RBD, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50878, RT_POL, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPRAPRCRAV RALLRASYRQ VLPLAAFVRR LRPQGHRLVR RGDPAAFRAL
60 70 80 90 100
VAQCLVCVPW DAQPPPAAPS FRQVSCLKEL VARVVQRLCE RGARNVLAFG
110 120 130 140 150
FTLLAGARGG PPVAFTTSVR SYLPNTVTDT LRGSGAWGLL LHRVGDDVLT
160 170 180 190 200
HLLSRCALYL LVPPTCAYQV CGPPLYDLRA AAAAARRPTR QVGGTRAGFG
210 220 230 240 250
LPRPASSNGG HGEAEGLLEA RAQGARRRRS SARGRLPPAK RPRRGLEPGR
260 270 280 290 300
DLEGQVARSP PRVVTPTRDA AEAKSRKGDV PGPCRLFPGG ERGVGSASWR
310 320 330 340 350
LSPSEGEPGA GACAETKRFL YCSGGGEQLR RSFLLCSLPP SLAGARTLVE
360 370 380 390 400
TIFLDSKPGP PGAPRRPRRL PARYWQMRPL FRKLLGNHAR SPYGALLRAH
410 420 430 440 450
CPLPASAPRA GPDHQKCPGV GGCPSERPAA APEGEANSGR LVQLLRQHSS
460 470 480 490 500
PWQVYGLLRA CLRRLVPAGL WGSRHNERRF LRNVKKLLSL GKHGRLSQQE
510 520 530 540 550
LTWKMKVQDC AWLRASPGAR CVPAAEHRQR EAVLGRFLHW LMGAYVVELL
560 570 580 590 600
RSFFYVTETT FQKNRLFFFR KRIWSQLQRL GVRQHLDRVR LRELSEAEVR
610 620 630 640 650
QHQEARPALL TSRLRFVPKP GGLRPIVNVG CVEGAPAPPR DKKVQHLSSR
660 670 680 690 700
VKTLFAVLNY ERARRPGLLG ASVLGMDDIH RAWRAFVLPL RARGPAPPLY
710 720 730 740 750
FVKVDVVGAY DALPQDKLAE VIANVLQPQE NTYCVRHCAM VRTARGRMRK
760 770 780 790 800
SFKRHVSTFS DFQPYLRQLV EHLQAMGSLR DAVVIEQSCS LNEPGSSLFN
810 820 830 840 850
LFLHLVRSHV IRIGGRSYIQ CQGIPQGSIL STLLCSFCYG DMENKLFPGV
860 870 880 890 900
QQDGVLLRLV DDFLLVTPHL TRARDFLRTL VRGVPEYGCQ VNLRKTVVNF
910 920 930 940 950
PVEPGALGGA APLQLPAHCL FPWCGLLLDT RTLEVHGDHS SYARTSIRAS
960 970 980 990 1000
LTFTQGFKPG RNMRRKLLAV LQLKCHGLFL DLQVNSLQTV FTNVYKIFLL
1010 1020 1030 1040 1050
QAYRFHACVL QLPFSQPVRS SPAFFLQVIA DTASRGYALL KARNAGASLG
1060 1070 1080 1090 1100
ARGAAGLFPS EAAQWLCLHA FLLKLARHRV TYSRLLGALR TARARLHRQL
1110 1120
PGPTRAALEA AADPALTADF KTILD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ399842 Genomic DNA Translation: ABD61652.2 |
RefSeqi | NP_001039707.1, NM_001046242.1 |
Genome annotation databases
GeneIDi | 518884 |
KEGGi | bta:518884 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ399842 Genomic DNA Translation: ABD61652.2 |
RefSeqi | NP_001039707.1, NM_001046242.1 |
3D structure databases
SMRi | Q27ID4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016685 |
Proteomic databases
PaxDbi | Q27ID4 |
PRIDEi | Q27ID4 |
Genome annotation databases
GeneIDi | 518884 |
KEGGi | bta:518884 |
Organism-specific databases
CTDi | 7015 |
Phylogenomic databases
eggNOGi | KOG1005, Eukaryota |
InParanoidi | Q27ID4 |
OrthoDBi | 1297956at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR000477, RT_dom IPR021891, Telomerase_RBD IPR003545, Telomerase_RT |
PANTHERi | PTHR12066, PTHR12066, 1 hit |
Pfami | View protein in Pfam PF00078, RVT_1, 1 hit PF12009, Telomerase_RBD, 1 hit |
PRINTSi | PR01365, TELOMERASERT |
SMARTi | View protein in SMART SM00975, Telomerase_RBD, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50878, RT_POL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TERT_BOVIN | |
Accessioni | Q27ID4Primary (citable) accession number: Q27ID4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | May 2, 2006 | |
Last modified: | December 2, 2020 | |
This is version 81 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families