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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Regulated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi200Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi623 – 654FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi767 – 778FADBy similarityAdd BLAST12
Nucleotide bindingi903 – 913FADBy similarityAdd BLAST11
Nucleotide bindingi978 – 996NADPBy similarityAdd BLAST19
Nucleotide bindingi1076 – 1091NADPBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandCalcium, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Short name:
NOSII
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001709261 – 1156Nitric oxide synthase, inducibleAdd BLAST1156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei575PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ27995
PRIDEiQ27995

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity). Interacts with GAPDH. Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009062

Structurei

3D structure databases

ProteinModelPortaliQ27995
SMRiQ27995
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini539 – 677Flavodoxin-likePROSITE-ProRule annotationAdd BLAST139
Domaini730 – 970FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST241

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni509 – 529Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
COG4362 LUCA
HOGENOMiHOG000220884
HOVERGENiHBG000159
InParanoidiQ27995
KOiK13241

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR012144 NOS_euk
IPR004030 NOS_N
IPR036119 NOS_N_sf
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF02898 NO_synthase, 1 hit
PIRSFiPIRSF000333 NOS, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF56512 SSF56512, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
PS60001 NOS, 1 hit

Sequencei

Sequence statusi: Complete.

Q27995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG
60 70 80 90 100
KHGNESPQPL TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL
110 120 130 140 150
HQKAKGDLSC KSKSCLASIM NPKSLTIGPR DKPTPPDELL PQAIEFVNQY
160 170 180 190 200
YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC
210 220 230 240 250
IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI RSAITVFPQR
260 270 280 290 300
SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG
310 320 330 340 350
RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP
360 370 380 390 400
AVANMLLEVG GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG
410 420 430 440 450
LETHKVASLW KDRAVVEINV AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE
460 470 480 490 500
YRSRGGCPAD WIWLVPPISG SITPVFHQEM LNYVLSPFYY YQVEPWKTHV
510 520 530 540 550
WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT ILFATETGRS
560 570 580 590 600
ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG
610 620 630 640 650
NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA
660 670 680 690 700
SQLAPTGEGD ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS
710 720 730 740 750
NVTWDPQHYR LVQDSEPLDL NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR
760 770 780 790 800
TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ PALVQGILER VVDGPAPHQP
810 820 830 840 850
VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ LLLRKLAQLA
860 870 880 890 900
TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL
910 920 930 940 950
PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS
960 970 980 990 1000
LKPQDPVPCF VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA
1010 1020 1030 1040 1050
EHKGLQGGRM TLVFGCRRPE EDHLYWEEML EMARKGVLHE VHTAYSRLPD
1060 1070 1080 1090 1100
QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY VCGDVRMARD VARTLKQLMA
1110 1120 1130 1140 1150
TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG AAGLPSNPRA

PGAHRS
Length:1,156
Mass (Da):131,208
Last modified:November 28, 2006 - v3
Checksum:i52A52C984462F692
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236N → S in AAC48470 (PubMed:7536776).Curated1
Sequence conflicti1068 – 1069VL → AF in AAC48479 (PubMed:7558036).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ676956 mRNA Translation: ABG74910.1
U14640 mRNA Translation: AAC48470.2
U18331 mRNA Translation: AAC48479.1
PIRiI46074
RefSeqiNP_001070267.1, NM_001076799.1
UniGeneiBt.23126

Genome annotation databases

GeneIDi282876
KEGGibta:282876

Similar proteinsi

Entry informationi

Entry nameiNOS2_BOVIN
AccessioniPrimary (citable) accession number: Q27995
Secondary accession number(s): Q06Q84, Q27985
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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