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UniProtKB - Q27974 (AUXI_BOVIN)
Protein
Putative tyrosine-protein phosphatase auxilin
Gene
DNAJC6
Organism
Bos taurus (Bovine)
Status
Functioni
Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles (PubMed:15502813).
Plays a role in clathrin-mediated endocytosis in neurons (By similarity).
By similarity1 PublicationCatalytic activityi
- EC:3.1.3.48
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 161 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- clathrin binding Source: CAFA
- clathrin heavy chain binding Source: CAFA
- protein domain specific binding Source: CAFA
- protein tyrosine phosphatase activity Source: UniProtKB-EC
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- clathrin coat disassembly Source: CAFA
- clathrin-dependent endocytosis Source: GO_Central
- synaptic vesicle uncoating Source: GO_Central
Keywordsi
Molecular function | Chaperone, Hydrolase, Protein phosphatase |
Names & Taxonomyi
Protein namesi | Recommended name: Putative tyrosine-protein phosphatase auxilin1 Publication (EC:3.1.3.48)Alternative name(s): DnaJ homolog subfamily C member 6By similarity |
Gene namesi | Name:DNAJC6By similarity |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
- postsynaptic density Source: GO_Central
- presynapse Source: GOC
- vesicle Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 847 | K → C: Strongly reduces interaction with HSPA8. 1 Publication | 1 | |
Mutagenesisi | 849 | K → C: Slightly reduces interaction with HSPA8. 1 Publication | 1 | |
Mutagenesisi | 876 | D → A: Loss of interaction with HSPA8. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215902 | 1 – 910 | Putative tyrosine-protein phosphatase auxilinAdd BLAST | 910 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 109 | PhosphoserineBy similarity | 1 | |
Modified residuei | 450 | PhosphoserineBy similarity | 1 | |
Modified residuei | 453 | PhosphoserineBy similarity | 1 | |
Modified residuei | 560 | PhosphoserineBy similarity | 1 | |
Modified residuei | 567 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.1 Publication
Target for coat-associated casein kinase II in vitro.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q27974 |
PRIDEi | Q27974 |
Expressioni
Tissue specificityi
Brain.1 Publication
Interactioni
Subunit structurei
Interacts with HSPA8/HSC70.
Interacts with clathrin heavy chains.
1 PublicationGO - Molecular functioni
- clathrin binding Source: CAFA
- clathrin heavy chain binding Source: CAFA
- protein domain specific binding Source: CAFA
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
DIPi | DIP-41952N |
ELMi | Q27974 |
IntActi | Q27974, 1 interactor |
MINTi | Q27974 |
STRINGi | 9913.ENSBTAP00000041032 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q27974 |
SMRi | Q27974 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q27974 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 33 – 36 | 1 | 4 | |
Repeati | 37 – 40 | 2 | 4 | |
Repeati | 41 – 44 | 3 | 4 | |
Domaini | 52 – 219 | Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST | 168 | |
Domaini | 225 – 363 | C2 tensin-typePROSITE-ProRule annotationAdd BLAST | 139 | |
Domaini | 846 – 910 | JPROSITE-ProRule annotationAdd BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 44 | 3 X 4 AA approximate tandem repeatsAdd BLAST | 12 | |
Regioni | 448 – 772 | DisorderedSequence analysisAdd BLAST | 325 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 406 – 414 | SH3-bindingSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 450 – 466 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 503 – 517 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 541 – 571 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 591 – 629 | Polar residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 650 – 674 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 722 – 759 | Polar residuesSequence analysisAdd BLAST | 38 |
Keywords - Domaini
Repeat, SH3-bindingPhylogenomic databases
eggNOGi | KOG0431, Eukaryota KOG2283, Eukaryota |
InParanoidi | Q27974 |
OrthoDBi | 826336at2759 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
DisProti | DP00351 |
Gene3Di | 1.10.287.110, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR035892, C2_domain_sf IPR001623, DnaJ_domain IPR036869, J_dom_sf IPR029021, Prot-tyrosine_phosphatase-like IPR014020, Tensin_C2-dom IPR029023, Tensin_phosphatase IPR000387, Tyr_Pase_dom |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF10409, PTEN_C2, 1 hit |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit SM01326, PTEN_C2, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49562, SSF49562, 1 hit SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS51182, C2_TENSIN, 1 hit PS50076, DNAJ_2, 1 hit PS51181, PPASE_TENSIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q27974-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSSGASSPD MEPSYGGGLF DMVKGGAGRL FSNLKDNLKD TLKDTSSRVI
60 70 80 90 100
QSVTSYTKGD LDFTYVTSRI IVMSFPLDSV DIGFRNQVDD IRSFLDSRHL
110 120 130 140 150
DHYTVYNLSP KSYRTAKFHS RVSECSWPIR QAPSLHNLFA VCRNMYNWLL
160 170 180 190 200
QNPKNVCVVH CLDGRAASSI LVGAMFIFCN LYSTPGPAVR LLYAKRPGIG
210 220 230 240 250
LSPSHRRYLG YMCDLLADKP YRPHFKPLTI KSITVSPVPF FNKQRNGCRP
260 270 280 290 300
YCDVLIGETK IYTTCADFER MKEYRVQDGK IFIPLSITVQ GDVVVSMYHL
310 320 330 340 350
RSTIGSRLQA KVTNTQIFQL QFHTGFIPLD TTVLKFTKPE LDACDVPEKY
360 370 380 390 400
PQLFQVTLDV ELQPHDKVME LTPPWEHYCT KDVNPSILFS SHQEHQDTLV
410 420 430 440 450
LGGQAPIDIP PDNPRHFGQG GFFSTLCWQD QKSEKSFCEE DHAALVNQES
460 470 480 490 500
EQSDDELLTL SSPHGNANGD KPHAARKPSK KQQEPAAPAP PEDVDLLGLE
510 520 530 540 550
GSAVSKNFSS PAAPPSNSEL LSDLFGGGGA AGPVQSGQSG VDDVFHPSGP
560 570 580 590 600
TSTQSTPRRS ATSTSASPTL RVGEGATFDP FGAPSKPSGQ DLLGSFLNTA
610 620 630 640 650
SASSDPFLQP TRSPSPTVHA SSTPAVNIQP DVSGAWDWHT KPGGFGMGSK
660 670 680 690 700
SAATSPTGSS HGTPTHQNKP QTLDPFADLG TLGGSSFASK PSTPTGLGGG
710 720 730 740 750
FPPLSSPQKA SPQPMGGGWQ QGGGYNWQQT QSKPQSSMPH SSPQNRPNYN
760 770 780 790 800
VSFSSMPGGQ NERGKAAANL EGKQKAADFE DLLSGQGFNA HKDKKGPRTI
810 820 830 840 850
AEMRKEEMAK EMDPEKLKIL EWIEGKERNI RALLSTMHTV LWAGETKWKP
860 870 880 890 900
VGMADLVTPE QVKKVYRKAV LVVHPDKATG QPYEQYAKMI FMELNDAWSE
910
FENQGQKPLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09237 mRNA Translation: AAA79037.1 |
PIRi | S68983 |
RefSeqi | NP_777261.1, NM_174836.2 |
Genome annotation databases
GeneIDi | 317659 |
KEGGi | bta:317659 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09237 mRNA Translation: AAA79037.1 |
PIRi | S68983 |
RefSeqi | NP_777261.1, NM_174836.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N4C | NMR | - | A | 737-910 | [»] | |
1NZ6 | X-ray | 2.50 | A/B | 810-910 | [»] | |
1XI5 | electron microscopy | 12.00 | J/K/L/M/N/O/P/Q/R | 797-910 | [»] | |
2QWN | X-ray | 2.40 | B | 812-905 | [»] | |
2QWO | X-ray | 1.70 | B | 813-904 | [»] | |
2QWP | X-ray | 1.75 | B | 813-904 | [»] | |
2QWQ | X-ray | 2.21 | B | 813-904 | [»] | |
2QWR | X-ray | 2.21 | B | 813-904 | [»] | |
3N0A | X-ray | 2.20 | A | 40-400 | [»] | |
BMRBi | Q27974 | |||||
SMRi | Q27974 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-41952N |
ELMi | Q27974 |
IntActi | Q27974, 1 interactor |
MINTi | Q27974 |
STRINGi | 9913.ENSBTAP00000041032 |
Proteomic databases
PaxDbi | Q27974 |
PRIDEi | Q27974 |
Genome annotation databases
GeneIDi | 317659 |
KEGGi | bta:317659 |
Organism-specific databases
CTDi | 9829 |
Phylogenomic databases
eggNOGi | KOG0431, Eukaryota KOG2283, Eukaryota |
InParanoidi | Q27974 |
OrthoDBi | 826336at2759 |
Miscellaneous databases
EvolutionaryTracei | Q27974 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
DisProti | DP00351 |
Gene3Di | 1.10.287.110, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR035892, C2_domain_sf IPR001623, DnaJ_domain IPR036869, J_dom_sf IPR029021, Prot-tyrosine_phosphatase-like IPR014020, Tensin_C2-dom IPR029023, Tensin_phosphatase IPR000387, Tyr_Pase_dom |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF10409, PTEN_C2, 1 hit |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit SM01326, PTEN_C2, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49562, SSF49562, 1 hit SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS51182, C2_TENSIN, 1 hit PS50076, DNAJ_2, 1 hit PS51181, PPASE_TENSIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AUXI_BOVIN | |
Accessioni | Q27974Primary (citable) accession number: Q27974 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references