We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q27889 (PP2B2_DROME)
Protein
Serine/threonine-protein phosphatase 2B catalytic subunit 2
Gene
Pp2B-14D
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
2 PublicationsCatalytic activityi
Cofactori
Protein has several cofactor binding sites:- Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
- Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 156 | IronBy similarity | 1 | |
Metal bindingi | 158 | IronBy similarity | 1 | |
Metal bindingi | 184 | IronBy similarity | 1 | |
Metal bindingi | 184 | ZincBy similarity | 1 | |
Metal bindingi | 216 | ZincBy similarity | 1 | |
Active sitei | 217 | Proton donorBy similarity | 1 | |
Metal bindingi | 265 | ZincBy similarity | 1 | |
Metal bindingi | 347 | ZincBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent protein serine/threonine phosphatase activity Source: FlyBase
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein phosphatase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine phosphatase activity Source: FlyBase
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- calcineurin-mediated signaling Source: GO_Central
- female meiotic nuclear division Source: FlyBase
- meiotic cell cycle Source: FlyBase
- protein dephosphorylation Source: FlyBase
- regulation of embryonic development Source: FlyBase
- sleep Source: FlyBase
- wing disc development Source: FlyBase
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase, Protein phosphatase |
Ligand | Calcium, Iron, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-2025928, Calcineurin activates NFAT R-DME-2871809, FCERI mediated Ca+2 mobilization R-DME-4086398, Ca2+ pathway R-DME-5607763, CLEC7A (Dectin-1) induces NFAT activation |
SignaLinki | Q27889 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 2B catalytic subunit 2 (EC:3.1.3.16)Alternative name(s): Calmodulin-dependent calcineurin A2 subunit |
Gene namesi | Name:Pp2B-14D ORF Names:CG9842 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011826, Pp2B-14D |
VEuPathDBi | VectorBase:FBgn0011826 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058831 | 1 – 570 | Serine/threonine-protein phosphatase 2B catalytic subunit 2Add BLAST | 570 |
Proteomic databases
PaxDbi | Q27889 |
PRIDEi | Q27889 |
Expressioni
Tissue specificityi
Expressed in CNS and PNS.1 Publication
Developmental stagei
Expressed both maternally and zygotically in embryos, larvae and adults.2 Publications
Gene expression databases
Bgeei | FBgn0011826, Expressed in female gonad and 39 other tissues |
ExpressionAtlasi | Q27889, baseline and differential |
Genevisiblei | Q27889, DM |
Interactioni
Subunit structurei
Interacts with sra in a complex that contains CanA-14F.
2 PublicationsBinary interactionsi
Q27889
With | #Exp. | IntAct |
---|---|---|
euc [Q9VE19] | 4 | EBI-132663,EBI-9936648 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 58962, 41 interactors |
DIPi | DIP-17529N |
IntActi | Q27889, 22 interactors |
STRINGi | 7227.FBpp0301546 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 88 | DisorderedSequence analysisAdd BLAST | 88 | |
Regioni | 530 – 570 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 67 | Polar residuesSequence analysisAdd BLAST | 67 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000154115 |
HOGENOMi | CLU_004962_6_0_1 |
InParanoidi | Q27889 |
OMAi | MESFCCL |
OrthoDBi | 463522at2759 |
PhylomeDBi | Q27889 |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
SUPFAMi | SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q27889-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSNNQSSSV AQAATSARTV SAGSAEATDA NSTASNNNNN SSSTAAAGNN
60 70 80 90 100
SDNSSPTTGT GTGASTGKLH GGHTAVNTKE RVVDSVPFPP SHKLTLAEVF
110 120 130 140 150
DQRTGKPNHE LLKQHFILEG RIEEAPALKI IQDGAALLRQ EKTMIDIEAP
160 170 180 190 200
VTVCGDIHGQ FYDLMKLFEV GGSPASTKYL FLGDYVDRGY FSIECVLYLW
210 220 230 240 250
SLKITYPQTL FLLRGNHECR HLTEYFTFKQ ECKIKYSERV YDACMDAFDC
260 270 280 290 300
LPLAALMNQQ FLCVHGGLSP EIHELEDIRR LDRFKEPPAF GPMCDLLWSD
310 320 330 340 350
PLEDFGNEKN SDFYTHNSVR GCSYFYSYAA CCDFLQNNNL LSIIRAHEAQ
360 370 380 390 400
DAGYRMYRKS QTTGFPSLIT IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF
410 420 430 440 450
NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM LVNVLNICSD DELMTEESEE
460 470 480 490 500
PLSDDEAALR KEVIRNKIRA IGKMARVFSV LREESESVLQ LKGLTPTGAL
510 520 530 540 550
PLGALSGGKQ SLKNAMQGFS PNHKITSFAE AKGLDAVNER MPPRRDQPPT
560 570
PSEDPNQHSQ QGGKNGAGHG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9NE01 | M9NE01_DROME | Serine/threonine-protein phosphatas... | Pp2B-14D calneur, CaN, CaNA, CanA, canA | 570 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 | Missing (PubMed:8849894).Curated | 1 | |
Sequence conflicti | 133 | D → E in CAA54807 (PubMed:8313960).Curated | 1 | |
Sequence conflicti | 175 | A → Q in CAA54807 (PubMed:8313960).Curated | 1 | |
Sequence conflicti | 175 | A → Q in AAB29893 (PubMed:8849894).Curated | 1 | |
Sequence conflicti | 323 – 333 | SYFYSYAACCD → CY in CAA54807 (PubMed:8313960).CuratedAdd BLAST | 11 | |
Sequence conflicti | 407 | Y → I in CAA54807 (PubMed:8313960).Curated | 1 | |
Sequence conflicti | 459 | L → V in CAA54807 (PubMed:8313960).Curated | 1 | |
Sequence conflicti | 491 | Missing in CAA54807 (PubMed:8313960).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77768 mRNA Translation: CAA54807.1 S68806 mRNA Translation: AAB29893.1 AE014298 Genomic DNA Translation: AAF48622.1 BT010083 mRNA Translation: AAQ22552.1 |
PIRi | S41743 S70551 |
RefSeqi | NP_001245715.1, NM_001258786.3 NP_001245716.1, NM_001258787.2 NP_523373.2, NM_078649.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0074294; FBpp0074069; FBgn0011826 FBtr0305593; FBpp0294044; FBgn0011826 FBtr0309793; FBpp0301546; FBgn0011826 |
GeneIDi | 32624 |
KEGGi | dme:Dmel_CG9842 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77768 mRNA Translation: CAA54807.1 S68806 mRNA Translation: AAB29893.1 AE014298 Genomic DNA Translation: AAF48622.1 BT010083 mRNA Translation: AAQ22552.1 |
PIRi | S41743 S70551 |
RefSeqi | NP_001245715.1, NM_001258786.3 NP_001245716.1, NM_001258787.2 NP_523373.2, NM_078649.4 |
3D structure databases
BMRBi | Q27889 |
SMRi | Q27889 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 58962, 41 interactors |
DIPi | DIP-17529N |
IntActi | Q27889, 22 interactors |
STRINGi | 7227.FBpp0301546 |
Proteomic databases
PaxDbi | Q27889 |
PRIDEi | Q27889 |
Protocols and materials databases
DNASUi | 32624 |
Genome annotation databases
EnsemblMetazoai | FBtr0074294; FBpp0074069; FBgn0011826 FBtr0305593; FBpp0294044; FBgn0011826 FBtr0309793; FBpp0301546; FBgn0011826 |
GeneIDi | 32624 |
KEGGi | dme:Dmel_CG9842 |
Organism-specific databases
CTDi | 32624 |
FlyBasei | FBgn0011826, Pp2B-14D |
VEuPathDBi | VectorBase:FBgn0011826 |
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000154115 |
HOGENOMi | CLU_004962_6_0_1 |
InParanoidi | Q27889 |
OMAi | MESFCCL |
OrthoDBi | 463522at2759 |
PhylomeDBi | Q27889 |
Enzyme and pathway databases
Reactomei | R-DME-2025928, Calcineurin activates NFAT R-DME-2871809, FCERI mediated Ca+2 mobilization R-DME-4086398, Ca2+ pathway R-DME-5607763, CLEC7A (Dectin-1) induces NFAT activation |
SignaLinki | Q27889 |
Miscellaneous databases
BioGRID-ORCSi | 32624, 0 hits in 1 CRISPR screen |
GenomeRNAii | 32624 |
PROi | PR:Q27889 |
Gene expression databases
Bgeei | FBgn0011826, Expressed in female gonad and 39 other tissues |
ExpressionAtlasi | Q27889, baseline and differential |
Genevisiblei | Q27889, DM |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
SUPFAMi | SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PP2B2_DROME | |
Accessioni | Q27889Primary (citable) accession number: Q27889 Secondary accession number(s): Q26248, Q9VXF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | August 30, 2005 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families