UniProtKB - Q27571 (NOS_DROME)
Nitric oxide synthase
Nos
Functioni
Catalyzes the conversion of L-arginine to L-citrulline producing nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7568075, PubMed:12804606).
Truncated isoforms (isoform 3-isoform 6) are able to form intracellular complexes with the full-length protein and serve as dominant negative inhibitors of the enzyme activity.
3 PublicationsCatalytic activityi
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxide1 Publication1 PublicationEC:1.14.13.391 Publication1 Publication
Cofactori
Protein has several cofactor binding sites:- hemeBy similarity
- FADBy similarityNote: Binds 1 FAD.By similarity
- FMNBy similarityNote: Binds 1 FMN.By similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 328 | Iron (heme axial ligand)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 814 – 845 | FMNPROSITE-ProRule annotationAdd BLAST | 32 | |
Nucleotide bindingi | 957 – 968 | FADBy similarityAdd BLAST | 12 | |
Nucleotide bindingi | 1100 – 1110 | FADBy similarityAdd BLAST | 11 | |
Nucleotide bindingi | 1175 – 1193 | NADPBy similarityAdd BLAST | 19 | |
Nucleotide bindingi | 1273 – 1287 | NADPBy similarityAdd BLAST | 15 |
GO - Molecular functioni
- calmodulin binding Source: FlyBase
- flavin adenine dinucleotide binding Source: GO_Central
- FMN binding Source: GO_Central
- heme binding Source: FlyBase
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- nitric-oxide synthase activity Source: FlyBase
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- arginine catabolic process Source: GO_Central
- imaginal disc development Source: FlyBase
- negative regulation of cell population proliferation Source: FlyBase
- negative regulation of DNA replication Source: FlyBase
- nervous system development Source: FlyBase
- nitric oxide biosynthetic process Source: GO_Central
- nitric oxide mediated signal transduction Source: GO_Central
- positive regulation of guanylate cyclase activity Source: GO_Central
- regulation of heart rate Source: FlyBase
- regulation of organ growth Source: FlyBase
- response to hormone Source: GO_Central
- response to lipopolysaccharide Source: GO_Central
- synapse assembly Source: FlyBase
Keywordsi
Molecular function | Calmodulin-binding, Oxidoreductase |
Ligand | FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
Reactomei | R-DME-1222556, ROS and RNS production in phagocytes R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-DME-203615, eNOS activation R-DME-203754, NOSIP mediated eNOS trafficking R-DME-392154, Nitric oxide stimulates guanylate cyclase R-DME-5218920, VEGFR2 mediated vascular permeability R-DME-5578775, Ion homeostasis R-DME-9009391, Extra-nuclear estrogen signaling R-DME-9033241, Peroxisomal protein import |
Names & Taxonomyi
Protein namesi | Recommended name: Nitric oxide synthase1 Publication (EC:1.14.13.391 Publication1 Publication)Short name: dNOS1 Publication |
Gene namesi | Name:Nos ORF Names:CG6713 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011676, Nos |
VEuPathDBi | VectorBase:FBgn0011676 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: GO_Central
Peroxisome
- peroxisome Source: FlyBase
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- vesicle membrane Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170951 | 1 – 1349 | Nitric oxide synthaseAdd BLAST | 1349 |
Proteomic databases
PaxDbi | Q27571 |
PRIDEi | Q27571 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0011676, Expressed in central nervous system and 18 other tissues |
Genevisiblei | Q27571, DM |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 60568, 5 interactors |
IntActi | Q27571, 2 interactors |
STRINGi | 7227.FBpp0099922 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 671 – 868 | Flavodoxin-likePROSITE-ProRule annotationAdd BLAST | 198 | |
Domaini | 928 – 1167 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 240 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 195 | DisorderedSequence analysisAdd BLAST | 173 | |
Regioni | 641 – 661 | Calmodulin-bindingSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 23 – 80 | Polar residuesSequence analysisAdd BLAST | 58 | |
Compositional biasi | 99 – 115 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 124 – 141 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 163 – 193 | Polar residuesSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
GeneTreei | ENSGT00940000168337 |
InParanoidi | Q27571 |
PhylomeDBi | Q27571 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit 3.90.1230.10, 1 hit 3.90.340.10, 1 hit 3.90.440.10, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR044943, NOS_dom_1 IPR044940, NOS_dom_2 IPR044944, NOS_dom_3 IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
s (7)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ
60 70 80 90 100
QNSRKIKTQA TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG
110 120 130 140 150
GLPSSSGTPL RHHKRASIST ASPPIRERRG TNTSIVVELD GSGSGSGSGG
160 170 180 190 200
GGVGVGQGAG CPPSGSCTAS GKSSRELSPS PKNQQQPRKM SQDYRSRAGS
210 220 230 240 250
FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL SCSKATCTSS
260 270 280 290 300
IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS
310 320 330 340 350
IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG
360 370 380 390 400
MFEAICNHIK YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ
410 420 430 440 450
ADGKIIGDPM NVEFTEVCTK LGWKSKGSEW DILPLVVSAN GHDPDYFDYP
460 470 480 490 500
PELILEVPLT HPKFEWFSDL GLRWYALPAV SSMLFDVGGI QFTATTFSGW
510 520 530 540 550
YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD KAVVEMNIAV
560 570 580 590 600
LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI
610 620 630 640 650
TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI
660 670 680 690 700
ARAVKFTSKL FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ
710 720 730 740 750
IYCMSDYDIS SIEHEALLIV VASTFGNGDP PENGELFSQE LYAMRVQESS
760 770 780 790 800
EHGLQDSSIG SSKSFMKASS RQEFMKLPLQ QVKRIDRWDS LRGSTSDTFT
810 820 830 840 850
EETFGPLSNV RFAVFALGSS AYPNFCAFGQ YVDNILGELG GERLLRVAYG
860 870 880 890 900
DEMCGQEQSF RKWAPEVFKL ACETFCLDPE ESLSDASLAL QNDSLTVNTV
910 920 930 940 950
RLVPSANKGS LDSSLSKYHN KKVHCCKAKA KPHNLTRLSE GAKTTMLLEI
960 970 980 990 1000
CAPGLEYEPG DHVGIFPANR TELVDGLLNR LVGVDNPDEV LQLQLLKEKQ
1010 1020 1030 1040 1050
TSNGIFKCWE PHDKIPPDTL RNLLARFFDL TTPPSRQLLT LLAGFCEDTA
1060 1070 1080 1090 1100
DKERLELLVN DSSAYEDWRH WRLPHLLDVL EEFPSCRPPA PLLLAQLTPL
1110 1120 1130 1140 1150
QPRFYSISSS PRRVSDEIHL TVAIVKYRCE DGQGDERYGV CSNYLSGLRA
1160 1170 1180 1190 1200
DDELFMFVRS ALGFHLPSDR SRPIILIGPG TGIAPFRSFW QEFQVLSDLD
1210 1220 1230 1240 1250
PTAKLPKMWL FFGCRNRDVD LYAEEKAELQ KDQILDRVFL ALSREQAIPK
1260 1270 1280 1290 1300
TYVQDLIEQE FDSLYQLIVQ ERGHIYVCGD VTMAEHVYQT IRKCIAGKEQ
1310 1320 1330 1340
KSEAEVETFL LTLRDESRYH EDIFGITLRT AEIHTKSRAT ARIRMASQP
The sequence of this isoform differs from the canonical sequence as follows:
758-1291: Missing.
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | S → A in AAC46882 (PubMed:7568075).Curated | 1 | |
Sequence conflicti | 24 | Q → QQ in AAC46882 (PubMed:7568075).Curated | 1 | |
Sequence conflicti | 105 | S → L in AAC46882 (PubMed:7568075).Curated | 1 | |
Sequence conflicti | 287 | S → C in AAC46882 (PubMed:7568075).Curated | 1 | |
Sequence conflicti | 373 | T → S in AAF25682 (PubMed:11526108).Curated | 1 | |
Sequence conflicti | 1197 | S → R in AAC46882 (PubMed:7568075).Curated | 1 | |
Sequence conflicti | 1197 | S → R in AAF25682 (PubMed:11526108).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003585 | 201 – 214 | FMHLD…SLLMR → RFFPARRPPVRAAL in isoform 3. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_003586 | 215 – 1349 | Missing in isoform 3. CuratedAdd BLAST | 1135 | |
Alternative sequenceiVSP_003587 | 417 – 521 | Missing in isoform 2. 1 PublicationAdd BLAST | 105 | |
Alternative sequenceiVSP_003590 | 701 – 746 | IYCMS…YAMRV → CPVGSVSSDPQDPVQLGLVV VLVSSTCVCKKHLPAAWQNK ISCKHL in isoform 6. CuratedAdd BLAST | 46 | |
Alternative sequenceiVSP_003588 | 701 – 704 | IYCM → VSLT in isoform 5. Curated | 4 | |
Alternative sequenceiVSP_003589 | 705 – 1349 | Missing in isoform 5. CuratedAdd BLAST | 645 | |
Alternative sequenceiVSP_003592 | 736 – 756 | LFSQE…HGLQD → VSTPPRKDHTELINGLGPAA F in isoform 4. CuratedAdd BLAST | 21 | |
Alternative sequenceiVSP_003591 | 747 – 1349 | Missing in isoform 6. CuratedAdd BLAST | 603 | |
Alternative sequenceiVSP_003593 | 757 – 1349 | Missing in isoform 4. CuratedAdd BLAST | 593 | |
Alternative sequenceiVSP_003594 | 758 – 1291 | Missing in isoform 10. CuratedAdd BLAST | 534 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25117 mRNA Translation: AAC46882.1 AF215700 , AF215691, AF215692, AF215693, AF215694, AF215695, AF215696, AF215697, AF215698, AF215699 Genomic DNA Translation: AAF25682.1 AE014134 Genomic DNA Translation: AAF53014.1 AE014134 Genomic DNA Translation: AAZ66450.1 AE014134 Genomic DNA Translation: AAZ66451.1 AE014134 Genomic DNA Translation: AAZ66452.2 Sequence problems. AE014134 Genomic DNA Translation: AAZ66453.1 AE014134 Genomic DNA Translation: AAZ66454.1 AE014134 Genomic DNA Translation: AAZ66455.1 AE014134 Genomic DNA Translation: AAZ66456.1 AE014134 Genomic DNA Translation: AAZ66457.1 BT010016 mRNA Translation: AAQ22485.1 |
PIRi | T13254 |
RefSeqi | NP_001027240.1, NM_001032069.2 [Q27571-4] NP_001027243.2, NM_001032072.2 NP_523541.2, NM_078817.4 [Q27571-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0080188; FBpp0079777; FBgn0011676 [Q27571-1] FBtr0100489; FBpp0099926; FBgn0011676 [Q27571-4] |
GeneIDi | 34495 |
KEGGi | dme:Dmel_CG6713 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25117 mRNA Translation: AAC46882.1 AF215700 , AF215691, AF215692, AF215693, AF215694, AF215695, AF215696, AF215697, AF215698, AF215699 Genomic DNA Translation: AAF25682.1 AE014134 Genomic DNA Translation: AAF53014.1 AE014134 Genomic DNA Translation: AAZ66450.1 AE014134 Genomic DNA Translation: AAZ66451.1 AE014134 Genomic DNA Translation: AAZ66452.2 Sequence problems. AE014134 Genomic DNA Translation: AAZ66453.1 AE014134 Genomic DNA Translation: AAZ66454.1 AE014134 Genomic DNA Translation: AAZ66455.1 AE014134 Genomic DNA Translation: AAZ66456.1 AE014134 Genomic DNA Translation: AAZ66457.1 BT010016 mRNA Translation: AAQ22485.1 |
PIRi | T13254 |
RefSeqi | NP_001027240.1, NM_001032069.2 [Q27571-4] NP_001027243.2, NM_001032072.2 NP_523541.2, NM_078817.4 [Q27571-1] |
3D structure databases
SMRi | Q27571 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 60568, 5 interactors |
IntActi | Q27571, 2 interactors |
STRINGi | 7227.FBpp0099922 |
Proteomic databases
PaxDbi | Q27571 |
PRIDEi | Q27571 |
Genome annotation databases
EnsemblMetazoai | FBtr0080188; FBpp0079777; FBgn0011676 [Q27571-1] FBtr0100489; FBpp0099926; FBgn0011676 [Q27571-4] |
GeneIDi | 34495 |
KEGGi | dme:Dmel_CG6713 |
Organism-specific databases
CTDi | 34495 |
FlyBasei | FBgn0011676, Nos |
VEuPathDBi | VectorBase:FBgn0011676 |
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
GeneTreei | ENSGT00940000168337 |
InParanoidi | Q27571 |
PhylomeDBi | Q27571 |
Enzyme and pathway databases
Reactomei | R-DME-1222556, ROS and RNS production in phagocytes R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-DME-203615, eNOS activation R-DME-203754, NOSIP mediated eNOS trafficking R-DME-392154, Nitric oxide stimulates guanylate cyclase R-DME-5218920, VEGFR2 mediated vascular permeability R-DME-5578775, Ion homeostasis R-DME-9009391, Extra-nuclear estrogen signaling R-DME-9033241, Peroxisomal protein import |
Miscellaneous databases
GenomeRNAii | 34495 |
PROi | PR:Q27571 |
Gene expression databases
Bgeei | FBgn0011676, Expressed in central nervous system and 18 other tissues |
Genevisiblei | Q27571, DM |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit 3.90.1230.10, 1 hit 3.90.340.10, 1 hit 3.90.440.10, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR044943, NOS_dom_1 IPR044940, NOS_dom_2 IPR044944, NOS_dom_3 IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NOS_DROME | |
Accessioni | Q27571Primary (citable) accession number: Q27571 Secondary accession number(s): A4V0K9 Q9VKP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families