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UniProtKB - Q27571 (NOS_DROME)

Entry version 187 (07 Oct 2020)
Sequence version 3 (10 Jun 2008)
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Protein

Nitric oxide synthase

Gene

Nos

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Truncated isoforms (isoform 3-isoform 6) are able to form intracellular complexes with the full-length protein and serve as dominant negative inhibitors of the enzyme activity.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Stimulated by calcium/calmodulin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi328Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi814 – 845FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi957 – 968FADBy similarityAdd BLAST12
Nucleotide bindingi1100 – 1110FADBy similarityAdd BLAST11
Nucleotide bindingi1175 – 1193NADPBy similarityAdd BLAST19
Nucleotide bindingi1273 – 1287NADPBy similarityAdd BLAST15

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-DME-1222556, ROS and RNS production in phagocytes
R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-203615, eNOS activation
R-DME-203754, NOSIP mediated eNOS trafficking
R-DME-392154, Nitric oxide stimulates guanylate cyclase
R-DME-5218920, VEGFR2 mediated vascular permeability
R-DME-5578775, Ion homeostasis
R-DME-9009391, Extra-nuclear estrogen signaling
R-DME-9033241, Peroxisomal protein import

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Alternative name(s):
dNOS
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Nos
ORF Names:CG6713
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2L

Organism-specific databases

Drosophila genome database

More...FlyBasei		FBgn0011676, Nos

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001709511 – 1349Nitric oxide synthaseAdd BLAST1349

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q27571

PRoteomics IDEntifications database

More...PRIDEi		Q27571

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Isoform 3 is expressed in larvae only. Isoform 4, isoform 5, isoform 6 and isoform 10 are expressed throughout development from embryos to adults.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		FBgn0011676, Expressed in central nervous system and 18 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q27571, DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		60568, 5 interactors

Protein interaction database and analysis system

More...IntActi		Q27571, 2 interactors

STRING: functional protein association networks

More...STRINGi		7227.FBpp0099922

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q27571

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini671 – 868Flavodoxin-likePROSITE-ProRule annotationAdd BLAST198
Domaini928 – 1167FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST240

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni641 – 661Calmodulin-bindingSequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi24 – 51Gln-richAdd BLAST28
Compositional biasi76 – 79Poly-Gly4
Compositional biasi145 – 156Poly-GlyAdd BLAST12

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1158, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000168337

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q27571

KEGG Orthology (KO)

More...KOi		K13240

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q27571

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003097, CysJ-like_FAD-binding
IPR017927, FAD-bd_FR_type
IPR001094, Flavdoxin-like
IPR008254, Flavodoxin/NO_synth
IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039, Flavoprotein-like_sf
IPR039261, FNR_nucleotide-bd
IPR023173, NADPH_Cyt_P450_Rdtase_alpha
IPR012144, NOS_euk
IPR004030, NOS_N
IPR036119, NOS_N_sf
IPR001433, OxRdtase_FAD/NAD-bd
IPR017938, Riboflavin_synthase-like_b-brl

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00667, FAD_binding_1, 1 hit
PF00258, Flavodoxin_1, 1 hit
PF00175, NAD_binding_1, 1 hit
PF02898, NO_synthase, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF000333, NOS, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00369, FLAVODOXIN
PR00371, FPNCR

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF52218, SSF52218, 1 hit
SSF52343, SSF52343, 1 hit
SSF56512, SSF56512, 1 hit
SSF63380, SSF63380, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51384, FAD_FR, 1 hit
PS50902, FLAVODOXIN_LIKE, 1 hit
PS60001, NOS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (7)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 7 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1Curated (identifier: Q27571-1) [UniParc]FASTAAdd to basket
Also known as: AImported, dNOS-1

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQHFTSIFE NLRFVTIKRS TNAQQQQQQQ QQQLQQQQQQ LQQQKAQTQQ 
        60         70         80         90        100
QNSRKIKTQA TPTLNGNGLL SGNPNGGGGD SSPSHEVDHP GGAQGAQAAG 
       110        120        130        140        150
GLPSSSGTPL RHHKRASIST ASPPIRERRG TNTSIVVELD GSGSGSGSGG 
       160        170        180        190        200
GGVGVGQGAG CPPSGSCTAS GKSSRELSPS PKNQQQPRKM SQDYRSRAGS 
       210        220        230        240        250
FMHLDDEGRS LLMRKPMRLK NIEGRPEVYD TLHCKGREIL SCSKATCTSS 
       260        270        280        290        300
IMNIGNAAVE ARKSDLILEH AKDFLEQYFT SIKRTSSTAH ETRWKQVRQS 
       310        320        330        340        350
IETTGHYQLT ETELIYGAKL AWRNSSRCIG RIQWSKLQVF DCRYVTTTSG 
       360        370        380        390        400
MFEAICNHIK YATNKGNLRS AITIFPQRTD AKHDYRIWNN QLISYAGYKQ 
       410        420        430        440        450
ADGKIIGDPM NVEFTEVCTK LGWKSKGSEW DILPLVVSAN GHDPDYFDYP 
       460        470        480        490        500
PELILEVPLT HPKFEWFSDL GLRWYALPAV SSMLFDVGGI QFTATTFSGW 
       510        520        530        540        550
YMSTEIGSRN LCDTNRRNML ETVALKMQLD TRTPTSLWKD KAVVEMNIAV 
       560        570        580        590        600
LHSYQSRNVT IVDHHTASES FMKHFENESK LRNGCPADWI WIVPPLSGSI 
       610        620        630        640        650
TPVFHQEMAL YYLKPSFEYQ DPAWRTHVWK KGRGESKGKK PRRKFNFKQI 
       660        670        680        690        700
ARAVKFTSKL FGRALSKRIK ATVLYATETG KSEQYAKQLC ELLGHAFNAQ 
       710        720        730        740        750
IYCMSDYDIS SIEHEALLIV VASTFGNGDP PENGELFSQE LYAMRVQESS 
       760        770        780        790        800
EHGLQDSSIG SSKSFMKASS RQEFMKLPLQ QVKRIDRWDS LRGSTSDTFT 
       810        820        830        840        850
EETFGPLSNV RFAVFALGSS AYPNFCAFGQ YVDNILGELG GERLLRVAYG 
       860        870        880        890        900
DEMCGQEQSF RKWAPEVFKL ACETFCLDPE ESLSDASLAL QNDSLTVNTV 
       910        920        930        940        950
RLVPSANKGS LDSSLSKYHN KKVHCCKAKA KPHNLTRLSE GAKTTMLLEI 
       960        970        980        990       1000
CAPGLEYEPG DHVGIFPANR TELVDGLLNR LVGVDNPDEV LQLQLLKEKQ 
      1010       1020       1030       1040       1050
TSNGIFKCWE PHDKIPPDTL RNLLARFFDL TTPPSRQLLT LLAGFCEDTA 
      1060       1070       1080       1090       1100
DKERLELLVN DSSAYEDWRH WRLPHLLDVL EEFPSCRPPA PLLLAQLTPL 
      1110       1120       1130       1140       1150
QPRFYSISSS PRRVSDEIHL TVAIVKYRCE DGQGDERYGV CSNYLSGLRA 
      1160       1170       1180       1190       1200
DDELFMFVRS ALGFHLPSDR SRPIILIGPG TGIAPFRSFW QEFQVLSDLD 
      1210       1220       1230       1240       1250
PTAKLPKMWL FFGCRNRDVD LYAEEKAELQ KDQILDRVFL ALSREQAIPK 
      1260       1270       1280       1290       1300
TYVQDLIEQE FDSLYQLIVQ ERGHIYVCGD VTMAEHVYQT IRKCIAGKEQ 
      1310       1320       1330       1340 
KSEAEVETFL LTLRDESRYH EDIFGITLRT AEIHTKSRAT ARIRMASQP
Length:1,349
Mass (Da):151,715
Last modified:June 10, 2008 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE19D8CBAEFAF76FC
GO
Isoform 2Curated (identifier: Q27571-2) [UniParc]FASTAAdd to basket
Also known as: dNOS-21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     417-521: Missing.

Show »
Length:1,244
Mass (Da):139,754
Checksum:iAD7AA548C03AE48F
GO
Isoform 3Curated (identifier: Q27571-3) [UniParc]FASTAAdd to basket
Also known as: dNOS-31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     201-214: FMHLDDEGRSLLMR → RFFPARRPPVRAAL
     215-1349: Missing.

Show »
Length:214
Mass (Da):22,460
Checksum:iAA970488F5BFBF76
GO
Isoform 4Curated (identifier: Q27571-4) [UniParc]FASTAAdd to basket
Also known as: B, dNOS-41 Publication, FImported

The sequence of this isoform differs from the canonical sequence as follows:
     736-756: LFSQELYAMRVQESSEHGLQD → VSTPPRKDHTELINGLGPAAF
     757-1349: Missing.

Show »
Length:756
Mass (Da):84,261
Checksum:i1ABA7993149272D0
GO
Isoform 5Curated (identifier: Q27571-5) [UniParc]FASTAAdd to basket
Also known as: dNOS-51 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     701-704: IYCM → VSLT
     705-1349: Missing.

Show »
Length:704
Mass (Da):78,690
Checksum:iEEF32F5F9BB8091E
GO
Isoform 6Curated (identifier: Q27571-6) [UniParc]FASTAAdd to basket
Also known as: dNOS-61 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     701-746: IYCMSDYDIS...FSQELYAMRV → CPVGSVSSDP...WQNKISCKHL
     747-1349: Missing.

Show »
Length:746
Mass (Da):83,144
Checksum:i8449B48E111D7132
GO
Isoform 10Curated (identifier: Q27571-7) [UniParc]FASTAAdd to basket
Also known as: dNOS-101 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     758-1291: Missing.

Show »
Length:815
Mass (Da):91,268
Checksum:i86C08065EBA6C062
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAZ66452 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti20S → A in AAC46882 (PubMed:7568075).Curated1
Sequence conflicti24Q → QQ in AAC46882 (PubMed:7568075).Curated1
Sequence conflicti105S → L in AAC46882 (PubMed:7568075).Curated1
Sequence conflicti287S → C in AAC46882 (PubMed:7568075).Curated1
Sequence conflicti373T → S in AAF25682 (PubMed:11526108).Curated1
Sequence conflicti1197S → R in AAC46882 (PubMed:7568075).Curated1
Sequence conflicti1197S → R in AAF25682 (PubMed:11526108).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_003585201 – 214FMHLD…SLLMR → RFFPARRPPVRAAL in isoform 3. CuratedAdd BLAST14
Alternative sequenceiVSP_003586215 – 1349Missing in isoform 3. CuratedAdd BLAST1135
Alternative sequenceiVSP_003587417 – 521Missing in isoform 2. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_003590701 – 746IYCMS…YAMRV → CPVGSVSSDPQDPVQLGLVV VLVSSTCVCKKHLPAAWQNK ISCKHL in isoform 6. CuratedAdd BLAST46
Alternative sequenceiVSP_003588701 – 704IYCM → VSLT in isoform 5. Curated4
Alternative sequenceiVSP_003589705 – 1349Missing in isoform 5. CuratedAdd BLAST645
Alternative sequenceiVSP_003592736 – 756LFSQE…HGLQD → VSTPPRKDHTELINGLGPAA F in isoform 4. CuratedAdd BLAST21
Alternative sequenceiVSP_003591747 – 1349Missing in isoform 6. CuratedAdd BLAST603
Alternative sequenceiVSP_003593757 – 1349Missing in isoform 4. CuratedAdd BLAST593
Alternative sequenceiVSP_003594758 – 1291Missing in isoform 10. CuratedAdd BLAST534

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U25117 mRNA Translation: AAC46882.1
AF215700 AF215699 Genomic DNA Translation: AAF25682.1
AE014134 Genomic DNA Translation: AAF53014.1
AE014134 Genomic DNA Translation: AAZ66450.1
AE014134 Genomic DNA Translation: AAZ66451.1
AE014134 Genomic DNA Translation: AAZ66452.2 Sequence problems.
AE014134 Genomic DNA Translation: AAZ66453.1
AE014134 Genomic DNA Translation: AAZ66454.1
AE014134 Genomic DNA Translation: AAZ66455.1
AE014134 Genomic DNA Translation: AAZ66456.1
AE014134 Genomic DNA Translation: AAZ66457.1
BT010016 mRNA Translation: AAQ22485.1

Protein sequence database of the Protein Information Resource

More...PIRi		T13254

NCBI Reference Sequences

More...RefSeqi		NP_001027240.1, NM_001032069.2 [Q27571-4]
NP_001027243.2, NM_001032072.2
NP_523541.2, NM_078817.4 [Q27571-1]

Genome annotation databases

Ensembl metazoan genome annotation project

More...EnsemblMetazoai		FBtr0080188; FBpp0079777; FBgn0011676 [Q27571-1]
FBtr0100489; FBpp0099926; FBgn0011676 [Q27571-4]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		34495

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		dme:Dmel_CG6713

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25117 mRNA Translation: AAC46882.1
AF215700 AF215699 Genomic DNA Translation: AAF25682.1
AE014134 Genomic DNA Translation: AAF53014.1
AE014134 Genomic DNA Translation: AAZ66450.1
AE014134 Genomic DNA Translation: AAZ66451.1
AE014134 Genomic DNA Translation: AAZ66452.2 Sequence problems.
AE014134 Genomic DNA Translation: AAZ66453.1
AE014134 Genomic DNA Translation: AAZ66454.1
AE014134 Genomic DNA Translation: AAZ66455.1
AE014134 Genomic DNA Translation: AAZ66456.1
AE014134 Genomic DNA Translation: AAZ66457.1
BT010016 mRNA Translation: AAQ22485.1
PIRiT13254
RefSeqiNP_001027240.1, NM_001032069.2 [Q27571-4]
NP_001027243.2, NM_001032072.2
NP_523541.2, NM_078817.4 [Q27571-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I86model-@217-629[»]
SMRiQ27571
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi60568, 5 interactors
IntActiQ27571, 2 interactors
STRINGi7227.FBpp0099922

Proteomic databases

PaxDbiQ27571
PRIDEiQ27571

Genome annotation databases

EnsemblMetazoaiFBtr0080188; FBpp0079777; FBgn0011676 [Q27571-1]
FBtr0100489; FBpp0099926; FBgn0011676 [Q27571-4]
GeneIDi34495
KEGGidme:Dmel_CG6713

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		34495
FlyBaseiFBgn0011676, Nos

Phylogenomic databases

eggNOGiKOG1158, Eukaryota
GeneTreeiENSGT00940000168337
InParanoidiQ27571
KOiK13240
PhylomeDBiQ27571

Enzyme and pathway databases

ReactomeiR-DME-1222556, ROS and RNS production in phagocytes
R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-203615, eNOS activation
R-DME-203754, NOSIP mediated eNOS trafficking
R-DME-392154, Nitric oxide stimulates guanylate cyclase
R-DME-5218920, VEGFR2 mediated vascular permeability
R-DME-5578775, Ion homeostasis
R-DME-9009391, Extra-nuclear estrogen signaling
R-DME-9033241, Peroxisomal protein import

Miscellaneous databases

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		34495

Protein Ontology

More...PROi		PR:Q27571

Gene expression databases

BgeeiFBgn0011676, Expressed in central nervous system and 18 other tissues
GenevisibleiQ27571, DM

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR003097, CysJ-like_FAD-binding
IPR017927, FAD-bd_FR_type
IPR001094, Flavdoxin-like
IPR008254, Flavodoxin/NO_synth
IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039, Flavoprotein-like_sf
IPR039261, FNR_nucleotide-bd
IPR023173, NADPH_Cyt_P450_Rdtase_alpha
IPR012144, NOS_euk
IPR004030, NOS_N
IPR036119, NOS_N_sf
IPR001433, OxRdtase_FAD/NAD-bd
IPR017938, Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667, FAD_binding_1, 1 hit
PF00258, Flavodoxin_1, 1 hit
PF00175, NAD_binding_1, 1 hit
PF02898, NO_synthase, 1 hit
PIRSFiPIRSF000333, NOS, 1 hit
PRINTSiPR00369, FLAVODOXIN
PR00371, FPNCR
SUPFAMiSSF52218, SSF52218, 1 hit
SSF52343, SSF52343, 1 hit
SSF56512, SSF56512, 1 hit
SSF63380, SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384, FAD_FR, 1 hit
PS50902, FLAVODOXIN_LIKE, 1 hit
PS60001, NOS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNOS_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q27571
Secondary accession number(s): A4V0K9
, A4V0L0, Q4ABG3, Q4ABG4, Q4ABG5, Q4ABG6, Q4ABG7, Q7YU33, Q9U096, Q9VKP8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 10, 2008
Last modified: October 7, 2020
This is version 187 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
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