UniProtKB - Q24572 (CAF1_DROME)
Chromatin assembly factor 1 p55 subunit
Caf1-55
Functioni
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the nucleosome remodeling and deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the nucleosome remodeling factor (NURF) complex, which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin; and the polycomb group (PcG) repressor complex ESC-E(Z), which promotes repression of homeotic genes during development. Also required for transcriptional repression of E2F target genes by E2f2 and Rbf or Rbf2.
5 PublicationsGO - Molecular functioni
- histone binding Source: FlyBase
- histone deacetylase binding Source: FlyBase
- protein homodimerization activity Source: FlyBase
- transcription cis-regulatory region binding Source: FlyBase
GO - Biological processi
- chromatin assembly Source: FlyBase
- chromatin remodeling Source: FlyBase
- eggshell chorion gene amplification Source: FlyBase
- facultative heterochromatin assembly Source: FlyBase
- heterochromatin assembly Source: FlyBase
- histone acetylation Source: FlyBase
- negative regulation of transcription, DNA-templated Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- nucleosome assembly Source: FlyBase
- nucleosome mobilization Source: FlyBase
- nucleosome positioning Source: FlyBase
- positive regulation of transcription, DNA-templated Source: FlyBase
- regulation of gene expression Source: GO_Central
- regulation of mitotic cell cycle Source: FlyBase
- segment specification Source: FlyBase
Keywordsi
Molecular function | Chromatin regulator, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-1538133, G0 and Early G1 R-DME-212300, PRC2 methylates histones and DNA R-DME-2559580, Oxidative Stress Induced Senescence R-DME-3214815, HDACs deacetylate histones R-DME-6804758, Regulation of TP53 Activity through Acetylation R-DME-8943724, Regulation of PTEN gene transcription R-DME-8951664, Neddylation |
SignaLinki | Q24572 |
Names & Taxonomyi
Protein namesi | Recommended name: Chromatin assembly factor 1 p55 subunit1 PublicationImportedShort name: CAF-1 p55 subunit1 Publication Alternative name(s): CAF-11 Publication Nucleosome-remodeling factor 55 kDa subunit1 Publication Short name: NURF-551 Publication |
Gene namesi | ORF Names:CG4236Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0263979, Caf1-55 |
VEuPathDBi | VectorBase:FBgn0263979 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Nucleus
- ESC/E(Z) complex Source: FlyBase
- histone methyltransferase complex Source: FlyBase
- Myb complex Source: FlyBase
- nucleus Source: FlyBase
- NuRD complex Source: FlyBase
- NURF complex Source: FlyBase
- Sin3-type complex Source: FlyBase
Other locations
- CAF-1 complex Source: FlyBase
- polytene chromosome Source: FlyBase
- transcription regulator complex Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050895 | 1 – 430 | Chromatin assembly factor 1 p55 subunitAdd BLAST | 430 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | Phosphoserine1 Publication | 1 | |
Modified residuei | 100 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q24572 |
PRIDEi | Q24572 |
PTM databases
iPTMneti | Q24572 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0263979, Expressed in embryo and 51 other tissues |
ExpressionAtlasi | Q24572, baseline and differential |
Genevisiblei | Q24572, DM |
Interactioni
Subunit structurei
Probably binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Self associates. Associates with chromatin.
Component of the CAF-1 complex, composed of Caf1-55, Caf1-105 and Caf1-180. Within the CAF-1 complex, Caf1-180 interacts directly with both Caf1-55 and Caf1-105.
Component of the NuRD complex, composed of at least Caf1-55, Mi-2, MTA1-like and HDAC1/Rpd3. Within the NuRD complex, Caf1-55 may interact directly with Mi-2, MTA1-like and HDAC1/Rpd3. The NuRD complex may also associate with the methyl-DNA binding protein MBD-like via Caf1-55 and Mi-2.
Component of the NURF complex, composed of Caf1-55, E(bx), Nurf-38 and Iswi.
Component of the Esc/E(z) complex, composed of Caf1-55, esc, E(z), Su(z)1, and possibly Pho1. The Esc/E(z) complex may also associate with Pcl and HDAC1/Rpd3 during early embryogenesis.
Interacts with Rbf and Rbf2.
Component of the DREAM complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l3mbt.
13 PublicationsBinary interactionsi
Q24572
With | #Exp. | IntAct |
---|---|---|
cid [Q9V6Q2] | 6 | EBI-75924,EBI-129287 |
E(z) [P42124] | 7 | EBI-75924,EBI-112315 |
esc [Q24338] | 11 | EBI-75924,EBI-88911 |
HDAC1 [Q94517] | 4 | EBI-75924,EBI-302197 |
GO - Molecular functioni
- histone binding Source: FlyBase
- histone deacetylase binding Source: FlyBase
- protein homodimerization activity Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 66900, 76 interactors |
DIPi | DIP-23697N |
IntActi | Q24572, 25 interactors |
MINTi | Q24572 |
STRINGi | 7227.FBpp0082511 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q24572 |
SMRi | Q24572 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q24572 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 126 – 159 | WD 1Add BLAST | 34 | |
Repeati | 179 – 210 | WD 2Add BLAST | 32 | |
Repeati | 229 – 260 | WD 3Add BLAST | 32 | |
Repeati | 275 – 306 | WD 4Add BLAST | 32 | |
Repeati | 319 – 350 | WD 5Add BLAST | 32 | |
Repeati | 376 – 407 | WD 6Add BLAST | 32 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0264, Eukaryota |
GeneTreei | ENSGT00940000154748 |
InParanoidi | Q24572 |
OMAi | PHEEGCL |
OrthoDBi | 831322at2759 |
PhylomeDBi | Q24572 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MVDRSDNAAE SFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT
60 70 80 90 100
AQWLPDVTKQ DGKDYSVHRL ILGTHTSDEQ NHLLIASVQL PSEDAQFDGS
110 120 130 140 150
HYDNEKGEFG GFGSVCGKIE IEIKINHEGE VNRARYMPQN ACVIATKTPS
160 170 180 190 200
SDVLVFDYTK HPSKPEPSGE CQPDLRLRGH QKEGYGLSWN PNLNGYLLSA
210 220 230 240 250
SDDHTICLWD INATPKEHRV IDAKNIFTGH TAVVEDVAWH LLHESLFGSV
260 270 280 290 300
ADDQKLMIWD TRNNNTSKPS HTVDAHTAEV NCLSFNPYSE FILATGSADK
310 320 330 340 350
TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLHVWD
360 370 380 390 400
LSKIGEEQST EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWIICSVSED
410 420 430
NIMQVWQMAE NVYNDEEPEI PASELETNTA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE1JIL4 | E1JIL4_DROME | Chromatin assembly factor 1, p55 su... | Caf1-55 154659_at, 48, 55, CAC3, CAF-1 | 429 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62388 mRNA Translation: AAB37257.1 AM294392 Genomic DNA Translation: CAL26322.1 AM294393 Genomic DNA Translation: CAL26323.1 AM294394 Genomic DNA Translation: CAL26324.1 AM294395 Genomic DNA Translation: CAL26325.1 AM294396 Genomic DNA Translation: CAL26330.1 AM294397 Genomic DNA Translation: CAL26335.1 AM294398 Genomic DNA Translation: CAL26337.1 AM294399 Genomic DNA Translation: CAL26338.1 AM294400 Genomic DNA Translation: CAL26339.1 AM294401 Genomic DNA Translation: CAL26340.1 AM294402 Genomic DNA Translation: CAL26341.1 AM294403 Genomic DNA Translation: CAL26342.1 AE014297 Genomic DNA Translation: AAF55146.1 AY061408 mRNA Translation: AAL28956.1 |
RefSeqi | NP_524354.1, NM_079630.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0083052; FBpp0082511; FBgn0263979 |
GeneIDi | 41836 |
KEGGi | dme:Dmel_CG4236 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62388 mRNA Translation: AAB37257.1 AM294392 Genomic DNA Translation: CAL26322.1 AM294393 Genomic DNA Translation: CAL26323.1 AM294394 Genomic DNA Translation: CAL26324.1 AM294395 Genomic DNA Translation: CAL26325.1 AM294396 Genomic DNA Translation: CAL26330.1 AM294397 Genomic DNA Translation: CAL26335.1 AM294398 Genomic DNA Translation: CAL26337.1 AM294399 Genomic DNA Translation: CAL26338.1 AM294400 Genomic DNA Translation: CAL26339.1 AM294401 Genomic DNA Translation: CAL26340.1 AM294402 Genomic DNA Translation: CAL26341.1 AM294403 Genomic DNA Translation: CAL26342.1 AE014297 Genomic DNA Translation: AAF55146.1 AY061408 mRNA Translation: AAL28956.1 |
RefSeqi | NP_524354.1, NM_079630.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XYI | X-ray | 1.75 | A | 1-430 | [»] | |
2YB8 | X-ray | 2.30 | B | 1-418 | [»] | |
2YBA | X-ray | 2.55 | A/B | 1-418 | [»] | |
3C99 | X-ray | 2.90 | A | 1-430 | [»] | |
3C9C | X-ray | 3.20 | A | 1-430 | [»] | |
AlphaFoldDBi | Q24572 | |||||
SMRi | Q24572 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 66900, 76 interactors |
DIPi | DIP-23697N |
IntActi | Q24572, 25 interactors |
MINTi | Q24572 |
STRINGi | 7227.FBpp0082511 |
PTM databases
iPTMneti | Q24572 |
Proteomic databases
PaxDbi | Q24572 |
PRIDEi | Q24572 |
Protocols and materials databases
DNASUi | 41836 |
Genome annotation databases
EnsemblMetazoai | FBtr0083052; FBpp0082511; FBgn0263979 |
GeneIDi | 41836 |
KEGGi | dme:Dmel_CG4236 |
Organism-specific databases
CTDi | 41836 |
FlyBasei | FBgn0263979, Caf1-55 |
VEuPathDBi | VectorBase:FBgn0263979 |
Phylogenomic databases
eggNOGi | KOG0264, Eukaryota |
GeneTreei | ENSGT00940000154748 |
InParanoidi | Q24572 |
OMAi | PHEEGCL |
OrthoDBi | 831322at2759 |
PhylomeDBi | Q24572 |
Enzyme and pathway databases
Reactomei | R-DME-1538133, G0 and Early G1 R-DME-212300, PRC2 methylates histones and DNA R-DME-2559580, Oxidative Stress Induced Senescence R-DME-3214815, HDACs deacetylate histones R-DME-6804758, Regulation of TP53 Activity through Acetylation R-DME-8943724, Regulation of PTEN gene transcription R-DME-8951664, Neddylation |
SignaLinki | Q24572 |
Miscellaneous databases
BioGRID-ORCSi | 41836, 1 hit in 3 CRISPR screens |
EvolutionaryTracei | Q24572 |
GenomeRNAii | 41836 |
PROi | PR:Q24572 |
Gene expression databases
Bgeei | FBgn0263979, Expressed in embryo and 51 other tissues |
ExpressionAtlasi | Q24572, baseline and differential |
Genevisiblei | Q24572, DM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAF1_DROME | |
Accessioni | Q24572Primary (citable) accession number: Q24572 Secondary accession number(s): A0AP04, Q9VFB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families