UniProtKB - Q24568 (NETB_DROME)
Protein
Netrin-B
Gene
NetB
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Netrins control guidance of CNS commissural axons and peripheral motor axons (PubMed:8780645, PubMed:8780646, PubMed:11719202). Its association with either fra or unc-5 receptors will lead to axon attraction or repulsion, respectively (PubMed:11719202). While short-range repulsion requires both fra and unc-5 receptors, long-range repulsion only requires unc-5 (PubMed:11719202).3 Publications
Caution
It is uncertain whether Met-1 or Met-9 is the initiator.Curated
GO - Biological processi
- animal organ morphogenesis Source: GO_Central
- axon guidance Source: FlyBase
- compound eye development Source: UniProtKB
- dendrite development Source: GO_Central
- dendrite guidance Source: FlyBase
- glial cell migration Source: FlyBase
- motor neuron axon guidance Source: FlyBase
- regulation of photoreceptor cell axon guidance Source: FlyBase
- salivary gland boundary specification Source: FlyBase
- synaptic target attraction Source: FlyBase
- synaptic target recognition Source: FlyBase
- tissue development Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Netrin-B |
Gene namesi | Name:NetB ORF Names:CG10521 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0015774, NetB |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix Curated
Other locations
- perinuclear region 1 Publication
Note: Expressed in the perinuclear region of the oldest most anterior lamina neurons at 24 hours after puparium formation.1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- axonal growth cone Source: UniProtKB
- basement membrane Source: GO_Central
- extracellular matrix Source: FlyBase
- neuronal cell body Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000017089 | 23 – 793 | Netrin-BAdd BLAST | 771 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 125 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 405 ↔ 414 | PROSITE-ProRule annotation | ||
Disulfide bondi | 407 ↔ 461 | PROSITE-ProRule annotation | ||
Disulfide bondi | 463 ↔ 472 | PROSITE-ProRule annotation | ||
Disulfide bondi | 475 ↔ 495 | PROSITE-ProRule annotation | ||
Disulfide bondi | 498 ↔ 507 | PROSITE-ProRule annotation | ||
Disulfide bondi | 500 ↔ 525 | PROSITE-ProRule annotation | ||
Disulfide bondi | 528 ↔ 537 | PROSITE-ProRule annotation | ||
Disulfide bondi | 540 ↔ 558 | PROSITE-ProRule annotation | ||
Disulfide bondi | 561 ↔ 573 | PROSITE-ProRule annotation | ||
Disulfide bondi | 563 ↔ 580 | PROSITE-ProRule annotation | ||
Disulfide bondi | 582 ↔ 591 | PROSITE-ProRule annotation | ||
Disulfide bondi | 594 ↔ 608 | PROSITE-ProRule annotation | ||
Disulfide bondi | 649 ↔ 738 | PROSITE-ProRule annotation | ||
Disulfide bondi | 652 ↔ 740 | PROSITE-ProRule annotation | ||
Disulfide bondi | 665 ↔ 792 | PROSITE-ProRule annotation | ||
Glycosylationi | 746 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q24568 |
PRIDEi | Q24568 |
Expressioni
Tissue specificityi
At 24 hr after puparium formation (APF), detected in the most anterior (oldest) L3, L4 and L5 lamina neurons (at protein level) (PubMed:29513217). At 48 hr APF, expressed in all L3, L4 and L5 neurons with slightly higher expression in the L3 neurons (at protein level) (PubMed:29513217). At the midline of developing CNS and in different subsets of neurons, muscles, and epidermal patches (PubMed:8780645, PubMed:8780646).3 Publications
Gene expression databases
Bgeei | FBgn0015774, Expressed in brain and 52 other tissues |
ExpressionAtlasi | Q24568, baseline and differential |
Genevisiblei | Q24568, DM |
Interactioni
Subunit structurei
Binds to unc-5 and fra receptors.
1 PublicationBinary interactionsi
Q24568
With | #Exp. | IntAct |
---|---|---|
fra [Q94538] | 2 | EBI-3406532,EBI-6895101 |
Protein-protein interaction databases
BioGRIDi | 58761, 6 interactors |
IntActi | Q24568, 890 interactors |
STRINGi | 7227.FBpp0291228 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 303 | Laminin N-terminalPROSITE-ProRule annotationAdd BLAST | 265 | |
Domaini | 405 – 497 | Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 498 – 560 | Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 561 – 610 | Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 649 – 792 | NTRPROSITE-ProRule annotationAdd BLAST | 144 |
Keywords - Domaini
Laminin EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG3512, Eukaryota |
GeneTreei | ENSGT00940000153882 |
InParanoidi | Q24568 |
OMAi | PSDCESH |
OrthoDBi | 858946at2759 |
PhylomeDBi | Q24568 |
Family and domain databases
Gene3Di | 2.40.50.120, 1 hit 2.60.120.1490, 2 hits |
InterProi | View protein in InterPro IPR002049, Laminin_EGF IPR008211, Laminin_N IPR038684, Laminin_N_sf IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00053, Laminin_EGF, 3 hits PF00055, Laminin_N, 1 hit PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00180, EGF_Lam, 3 hits SM00136, LamNT, 1 hit |
SUPFAMi | SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01248, EGF_LAM_1, 3 hits PS50027, EGF_LAM_2, 3 hits PS51117, LAMININ_NTER, 1 hit PS50189, NTR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q24568-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVRATGTRMG LLLPIILALA IGSSAAGISS NDPCYFEGKP RKCLPSFVNA
60 70 80 90 100
AYGNPVQASS VCGAQQPERY CELLRDGNAG ECRSCEQQRY GPAALTDLNN
110 120 130 140 150
PSNVTCWRSG AVNVPHDPDS APPDNVTLTL SLGKKYELTY ISLSFCPRSP
160 170 180 190 200
RPDSLAIFKS SDFGQTWQPF QFYSSQCQKF YGRPDRAKIS KFNEQEARCI
210 220 230 240 250
NSQHDTGGAA QRFAFNTLEG RPSANDLDSS LVLQDWVTAT DIRVVFHRLE
260 270 280 290 300
LPPQLLKVKN ANAFSDEMGG SREEDEDDDA DLELDGEQDE YDYNLQDNDS
310 320 330 340 350
ADAGYDEYEE PKKHLELDDD HLHLDYASDG ESVVKRQGKH KGSAYEKHYQ
360 370 380 390 400
SKLAATTPPQ QPPKVTPPGK VTPPSTAAPS AAASAVTLPI SQHYAVSDFA
410 420 430 440 450
VGGRCKCNGH ASECVATVSS GSGTALSDQD DGQDEDTPSA PSLANHFGRS
460 470 480 490 500
TQMSAKLTMT CACKHNTAGP ECERCKPFYF DRPWGRATDN DANECKMCQC
510 520 530 540 550
NGHARRCRFN LELYKLSGRV SGGVCYNCQH DTTGRYCHYC REGYYRDATK
560 570 580 590 600
PPNHRKVCKR CDCHPVGSTG KTCNHLSGQC PCKEGVTGLT CNRCARGYQQ
610 620 630 640 650
TRSHVAPCIK VPTNANMIQA ESAGGGGGGG TGDYKDGGGS QVEEMKKYCG
660 670 680 690 700
KCKASPKKLN LNKFCMEDYA ILAKVIGHDR ASQDISTEKF SIERQNEIYK
710 720 730 740 750
YEINIQTIFK RNPMSGTTSS LLGRGNMMLL VPRKSIECQC PKIKLNKSYL
760 770 780 790
ILGRDSEAAP GYLAIGPSSV VLEWKDEWSL RMKRFQRRAR KCS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JJP7 | E1JJP7_DROME | Netrin-B, isoform B | NetB CG15021, CT29512, Dmel\CG10521, net, NETB | 793 | Annotation score: | ||
M9PH47 | M9PH47_DROME | Netrin-B, isoform G | NetB CG15021, CT29512, Dmel\CG10521, net, NETB | 785 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60317 mRNA Translation: AAB17534.1 U63737 mRNA Translation: AAB17548.1 AE014298 Genomic DNA Translation: AAF48382.1 |
RefSeqi | NP_001162753.1, NM_001169282.1 NP_001162754.1, NM_001169283.2 NP_001162755.1, NM_001169284.2 NP_001245668.1, NM_001258739.2 NP_001245669.1, NM_001258740.2 NP_511155.1, NM_078600.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0073940; FBpp0073757; FBgn0015774 FBtr0302016; FBpp0291226; FBgn0015774 FBtr0302017; FBpp0291227; FBgn0015774 FBtr0302018; FBpp0291228; FBgn0015774 FBtr0304874; FBpp0293414; FBgn0015774 FBtr0304875; FBpp0293415; FBgn0015774 |
GeneIDi | 32400 |
KEGGi | dme:Dmel_CG10521 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60317 mRNA Translation: AAB17534.1 U63737 mRNA Translation: AAB17548.1 AE014298 Genomic DNA Translation: AAF48382.1 |
RefSeqi | NP_001162753.1, NM_001169282.1 NP_001162754.1, NM_001169283.2 NP_001162755.1, NM_001169284.2 NP_001245668.1, NM_001258739.2 NP_001245669.1, NM_001258740.2 NP_511155.1, NM_078600.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 58761, 6 interactors |
IntActi | Q24568, 890 interactors |
STRINGi | 7227.FBpp0291228 |
Proteomic databases
PaxDbi | Q24568 |
PRIDEi | Q24568 |
Genome annotation databases
EnsemblMetazoai | FBtr0073940; FBpp0073757; FBgn0015774 FBtr0302016; FBpp0291226; FBgn0015774 FBtr0302017; FBpp0291227; FBgn0015774 FBtr0302018; FBpp0291228; FBgn0015774 FBtr0304874; FBpp0293414; FBgn0015774 FBtr0304875; FBpp0293415; FBgn0015774 |
GeneIDi | 32400 |
KEGGi | dme:Dmel_CG10521 |
Organism-specific databases
CTDi | 32400 |
FlyBasei | FBgn0015774, NetB |
Phylogenomic databases
eggNOGi | KOG3512, Eukaryota |
GeneTreei | ENSGT00940000153882 |
InParanoidi | Q24568 |
OMAi | PSDCESH |
OrthoDBi | 858946at2759 |
PhylomeDBi | Q24568 |
Miscellaneous databases
BioGRID-ORCSi | 32400, 0 hits in 3 CRISPR screens |
GenomeRNAii | 32400 |
PROi | PR:Q24568 |
Gene expression databases
Bgeei | FBgn0015774, Expressed in brain and 52 other tissues |
ExpressionAtlasi | Q24568, baseline and differential |
Genevisiblei | Q24568, DM |
Family and domain databases
Gene3Di | 2.40.50.120, 1 hit 2.60.120.1490, 2 hits |
InterProi | View protein in InterPro IPR002049, Laminin_EGF IPR008211, Laminin_N IPR038684, Laminin_N_sf IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00053, Laminin_EGF, 3 hits PF00055, Laminin_N, 1 hit PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00180, EGF_Lam, 3 hits SM00136, LamNT, 1 hit |
SUPFAMi | SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01248, EGF_LAM_1, 3 hits PS50027, EGF_LAM_2, 3 hits PS51117, LAMININ_NTER, 1 hit PS50189, NTR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NETB_DROME | |
Accessioni | Q24568Primary (citable) accession number: Q24568 Secondary accession number(s): Q9VY23 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase