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Protein

Replication protein A 70 kDa DNA-binding subunit

Gene

RpA-70

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi179 – 252OBAdd BLAST74
Zinc fingeri464 – 486C4-typeSequence analysisAdd BLAST23

GO - Molecular functioni

  • damaged DNA binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: GO_Central
  • single-stranded DNA binding Source: FlyBase
  • single-stranded telomeric DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-110312 Translesion synthesis by REV1
R-DME-110314 Recognition of DNA damage by PCNA-containing replication complex
R-DME-110320 Translesion Synthesis by POLH
R-DME-176187 Activation of ATR in response to replication stress
R-DME-3108214 SUMOylation of DNA damage response and repair proteins
R-DME-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-DME-5655862 Translesion synthesis by POLK
R-DME-5656121 Translesion synthesis by POLI
R-DME-5656169 Termination of translesion DNA synthesis
R-DME-5696395 Formation of Incision Complex in GG-NER
R-DME-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-DME-5696400 Dual Incision in GG-NER
R-DME-6782135 Dual incision in TC-NER
R-DME-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-DME-68962 Activation of the pre-replicative complex
R-DME-69166 Removal of the Flap Intermediate

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein A 70 kDa DNA-binding subunit
Short name:
RP-A p70
Alternative name(s):
Replication factor A protein 1
Short name:
RF-A protein 1
Single-stranded DNA-binding protein
Short name:
DmRPA1
Gene namesi
Name:RpA-70
Synonyms:RPA1
ORF Names:CG9633
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010173 RpA-70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972591 – 603Replication protein A 70 kDa DNA-binding subunitAdd BLAST603

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei160Phosphoserine1 Publication1
Modified residuei420Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24492
PRIDEiQ24492

PTM databases

iPTMnetiQ24492

Expressioni

Gene expression databases

BgeeiFBgn0010173 Expressed in 61 organ(s), highest expression level in embryo
ExpressionAtlasiQ24492 baseline and differential
GenevisibleiQ24492 DM

Interactioni

Subunit structurei

Component of the heterotrimeric canonical replication protein A complex (RPA).By similarity

Protein-protein interaction databases

BioGridi66153, 15 interactors
DIPiDIP-22335N
IntActiQ24492, 5 interactors
STRINGi7227.FBpp0081356

Structurei

3D structure databases

ProteinModelPortaliQ24492
SMRiQ24492
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri464 – 486C4-typeSequence analysisAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0851 Eukaryota
COG1599 LUCA
GeneTreeiENSGT00390000012403
InParanoidiQ24492
KOiK07466
OMAiNIYLMDT
OrthoDBiEOG091G02VJ
PhylomeDBiQ24492

Family and domain databases

CDDicd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1
PfamiView protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SUPFAMiSSF50249 SSF50249, 4 hits
TIGRFAMsiTIGR00617 rpa1, 1 hit

Sequencei

Sequence statusi: Complete.

Q24492-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLASLSTGV IARIMHGEVV DAPVLQILAI KKINSAADSE RYRILISDGK
60 70 80 90 100
YFNSYAMLAS QLNVMQHNGE LEEFTIVQLD KYVTSLVGKD GAGKRVLIIS
110 120 130 140 150
ELTVVNPGAE VKSKIGEPVT YENAAKQDLA PKPAVTSNSK PIAKKEPSHN
160 170 180 190 200
NNNNIVMNSS INSGMTHPIS SLSPYQNKWV IKARVTSKSG IRTWSNARGE
210 220 230 240 250
GKLFSMDLMD ESGEIRATAF KEQCDKFYDL IQVDSVYYIS KCQLKPANKQ
260 270 280 290 300
YSSLNNAYEM TFSGETVVQL CEDTDDDPIP EIKYNLVPIS DVSGMENKAA
310 320 330 340 350
VDTIGICKEV GELQSFVART TNKEFKKRDI TLVDMSNSAI SLTLWGDDAV
360 370 380 390 400
NFDGHVQPVI LVKGTRINEF NGGKSLSLGG GSIMKINPDI PEAHKLRGWF
410 420 430 440 450
DNGGGDSVAN MVSARTGGGS FSTEWMTLKD ARARNLGSGD KPDYFQCKAV
460 470 480 490 500
VHIVKQENAF YRACPQSDCN KKVVDEGNDQ FRCEKCNALF PNFKYRLLIN
510 520 530 540 550
MSIGDWTSNR WVSSFNEVGE QLLGHTSQEV GEALENDPAK AEQIFSALNF
560 570 580 590 600
TSHIFKLRCK NEVYGDMTRN KLTVQSVAPI NHKEYNKHLL KELQELTGIG

SSN
Length:603
Mass (Da):66,624
Last modified:November 1, 1996 - v1
Checksum:i6E6E1D5C4FF38A95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70277 mRNA Translation: CAA94241.1
AE014297 Genomic DNA Translation: AAF54206.1
AY069331 mRNA Translation: AAL39476.1
RefSeqiNP_524274.1, NM_079550.4
UniGeneiDm.2177

Genome annotation databases

EnsemblMetazoaiFBtr0081867; FBpp0081356; FBgn0010173
GeneIDi40972
KEGGidme:Dmel_CG9633

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70277 mRNA Translation: CAA94241.1
AE014297 Genomic DNA Translation: AAF54206.1
AY069331 mRNA Translation: AAL39476.1
RefSeqiNP_524274.1, NM_079550.4
UniGeneiDm.2177

3D structure databases

ProteinModelPortaliQ24492
SMRiQ24492
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66153, 15 interactors
DIPiDIP-22335N
IntActiQ24492, 5 interactors
STRINGi7227.FBpp0081356

PTM databases

iPTMnetiQ24492

Proteomic databases

PaxDbiQ24492
PRIDEiQ24492

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081867; FBpp0081356; FBgn0010173
GeneIDi40972
KEGGidme:Dmel_CG9633

Organism-specific databases

CTDi40972
FlyBaseiFBgn0010173 RpA-70

Phylogenomic databases

eggNOGiKOG0851 Eukaryota
COG1599 LUCA
GeneTreeiENSGT00390000012403
InParanoidiQ24492
KOiK07466
OMAiNIYLMDT
OrthoDBiEOG091G02VJ
PhylomeDBiQ24492

Enzyme and pathway databases

ReactomeiR-DME-110312 Translesion synthesis by REV1
R-DME-110314 Recognition of DNA damage by PCNA-containing replication complex
R-DME-110320 Translesion Synthesis by POLH
R-DME-176187 Activation of ATR in response to replication stress
R-DME-3108214 SUMOylation of DNA damage response and repair proteins
R-DME-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-DME-5655862 Translesion synthesis by POLK
R-DME-5656121 Translesion synthesis by POLI
R-DME-5656169 Termination of translesion DNA synthesis
R-DME-5696395 Formation of Incision Complex in GG-NER
R-DME-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-DME-5696400 Dual Incision in GG-NER
R-DME-6782135 Dual incision in TC-NER
R-DME-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-DME-68962 Activation of the pre-replicative complex
R-DME-69166 Removal of the Flap Intermediate

Miscellaneous databases

GenomeRNAii40972
PROiPR:Q24492

Gene expression databases

BgeeiFBgn0010173 Expressed in 61 organ(s), highest expression level in embryo
ExpressionAtlasiQ24492 baseline and differential
GenevisibleiQ24492 DM

Family and domain databases

CDDicd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1
PfamiView protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SUPFAMiSSF50249 SSF50249, 4 hits
TIGRFAMsiTIGR00617 rpa1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRFA1_DROME
AccessioniPrimary (citable) accession number: Q24492
Secondary accession number(s): Q9VHU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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