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Protein

Polycomb protein Pcl

Gene

Pcl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required. Required for the correct spatial expression of the homeotic genes of the Antennapedia and Bithorax complexes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri424 – 472PHD-type 1PROSITE-ProRule annotationCuratedAdd BLAST49
Zinc fingeri512 – 560PHD-type 2PROSITE-ProRule annotationCuratedAdd BLAST49

GO - Molecular functioni

  • chromatin binding Source: FlyBase
  • DNA binding Source: UniProtKB
  • DNA binding transcription factor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin organization Source: UniProtKB
  • defense response to fungus Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-212300 PRC2 methylates histones and DNA

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb protein Pcl
Alternative name(s):
Polycomblike protein
Gene namesi
Name:Pcl
ORF Names:CG5109
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003044 Pcl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi517C → S or A: Abolishes interaction with E(z). 1 Publication1
Mutagenesisi527 – 530MLQC → QQQA: Abolishes interaction with E(z). 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593391 – 1043Polycomb protein PclAdd BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei805Phosphoserine1 Publication1
Modified residuei806Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24459
PRIDEiQ24459

PTM databases

iPTMnetiQ24459

Expressioni

Developmental stagei

Ubiquitous expression in embryos.1 Publication

Gene expression databases

BgeeiFBgn0003044
ExpressionAtlasiQ24459 baseline and differential
GenevisibleiQ24459 DM

Interactioni

Subunit structurei

Component of a form of the Esc/E(z) complex present specifically during early embryogenesis which is composed of Caf1, esc, E(z), Su(z)12, Pcl and Rpd3. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi62752, 24 interactors
IntActiQ24459, 24 interactors
MINTiQ24459
STRINGi7227.FBpp0303033

Structurei

Secondary structure

11043
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi341 – 345Combined sources5
Beta strandi356 – 360Combined sources5
Beta strandi366 – 374Combined sources9
Beta strandi379 – 383Combined sources5
Beta strandi388 – 391Combined sources4
Turni393 – 395Combined sources3
Turni502 – 504Combined sources3
Beta strandi508 – 511Combined sources4
Turni523 – 526Combined sources4
Beta strandi527 – 530Combined sources4
Turni531 – 533Combined sources3
Beta strandi536 – 539Combined sources4
Beta strandi542 – 544Combined sources3
Beta strandi556 – 559Combined sources4
Turni561 – 566Combined sources6
Beta strandi569 – 573Combined sources5
Helixi577 – 591Combined sources15
Beta strandi595 – 598Combined sources4
Turni599 – 602Combined sources4
Helixi603 – 610Combined sources8
Turni611 – 613Combined sources3
Helixi625 – 638Combined sources14
Turni639 – 642Combined sources4
Beta strandi643 – 647Combined sources5
Beta strandi653 – 661Combined sources9
Helixi679 – 686Combined sources8

3D structure databases

ProteinModelPortaliQ24459
SMRiQ24459
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ24459

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini349 – 404TudorAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi237 – 256Ser-richAdd BLAST20
Compositional biasi278 – 347Pro-richAdd BLAST70

Domaini

The PHD-type zinc fingers mediate the interaction with E(z).
In contrast to vertebrate homologs (PHF1, PHF19 and MTF2), the Tudor domain does not bind H3K36me3 (PubMed:23104054 and PubMed:23142980).

Sequence similaritiesi

Belongs to the Polycomblike family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri424 – 472PHD-type 1PROSITE-ProRule annotationCuratedAdd BLAST49
Zinc fingeri512 – 560PHD-type 2PROSITE-ProRule annotationCuratedAdd BLAST49

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4323 Eukaryota
ENOG410XQ6E LUCA
GeneTreeiENSGT00390000009222
InParanoidiQ24459
KOiK11485
OMAiRCAKPMK
OrthoDBiEOG091G01JL
PhylomeDBiQ24459

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR025894 Mtf2_C_dom
IPR002999 Tudor
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF14061 Mtf2_C, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00333 TUDOR, 1 hit
SUPFAMiSSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 2 hits
PS50016 ZF_PHD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q24459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNNHFHLQH DHPPQNVAHP FMQQPSTAVP SAPPATYGYL AQPAGQQPQW
60 70 80 90 100
MTTTYQILPP SVGPATVAKR YYATTGPQTT HPTHPSTIQI TNSFAQQSTP
110 120 130 140 150
PKQQAATSCS PFKANNIRII STAPSVYSLN KPPQEAHSTY APVQSYYLPS
160 170 180 190 200
GGGQTAGQIN LLAASGTGKQ LQPPPLVPVT NSTSPPSTVV LDRINICINN
210 220 230 240 250
HYTETPTSLS SSLTTAQQPS PIIPAIQHKA ILPLIDSSTA DSSSCSSSSV
260 270 280 290 300
SSSSYSGTAT TSAAVVIVDE PDSTTTTPQT PPTTPEAMSS PGKSSPSPPL
310 320 330 340 350
LATQSLLKGV NSMKPSFKTV EAAPPTPPTP PSPPPPPPAP PVAAPSPAVT
360 370 380 390 400
YALQEDVFIK CNDGRFYLGT IIDQTSDQYL IRFDDQSEQW CEPDKLRKLG
410 420 430 440 450
GGSSITAGGG GASTTESTNT SPSGPMCVAC KRSDIEDVVE ICERCGRGYH
460 470 480 490 500
RGCTVEIVTG SGIWSCKRCA KPMKMQQPVS HKITKPAGIC RQLPYHADKL
510 520 530 540 550
SWDEKHRVNE EQIYCYCGKP GKFDHNMLQC CKCRNWFHTQ CMQNFKKKLL
560 570 580 590 600
RGDMFFVFCC TVCNNGIEFV RRMQIEWVDV LHIALYNLRK HQHQKYHHLL
610 620 630 640 650
NDIWPFILEQ RHQLPICEKW RTLPETALME RLKQTLKDYS DRFVCGREFK
660 670 680 690 700
RAPAFYALRH SGPPHIPKVF LEPHEELSDE LLEKRFKLML MPEEPDEGAN
710 720 730 740 750
ELPKRVPKDV YEFNTDEDDP VETSEDEIPI KQIIEKAKKQ AAQKADKHDE
760 770 780 790 800
LPLKPDLADD NANDGDPGKL PAPIPPLLDA NSSRKRKAFR LSKRYDNSRN
810 820 830 840 850
HCDLSSDENS SSSRGTSSLD LIIPPPVNFL GRNNPFLMAT PKKASQGRSI
860 870 880 890 900
SVGTGVGVNG IINSIFKLKG TSKEQPRMVR TIKRRLSAKD ITIGPNQEVR
910 920 930 940 950
RRRTRRLTTA IEVISTTTIN PIPSHYLPIY AKDLQPPAPP MGKPTHGRLL
960 970 980 990 1000
RQRPQKQSPS QSRRNSTSST ATSSSSNGIG APGHSMLDLK QSVNKYFGGA
1010 1020 1030 1040
MNRIDAGEPF AIRAKRRMGN GQVQYLVEWG GDTATTAIGL LGN
Length:1,043
Mass (Da):114,638
Last modified:May 10, 2004 - v2
Checksum:iBD3325D7835EEAC3
GO

Sequence cautioni

The sequence AAA64457 differs from that shown. Reason: Frameshift at position 807.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93S → N in AAA64457 (PubMed:7956837).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35153 mRNA Translation: AAA64457.1 Frameshift.
AE013599 Genomic DNA Translation: AAF57748.2
AY075585 mRNA Translation: AAL68389.1
RefSeqiNP_001261067.1, NM_001274138.2
NP_476672.1, NM_057324.6
UniGeneiDm.4284

Genome annotation databases

EnsemblMetazoaiFBtr0086735; FBpp0085914; FBgn0003044
FBtr0329998; FBpp0303033; FBgn0003044
GeneIDi37069
KEGGidme:Dmel_CG5109
UCSCiCG5109-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiPCL_DROME
AccessioniPrimary (citable) accession number: Q24459
Secondary accession number(s): Q8T8P9, Q9V8C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: May 23, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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