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Protein

Alpha-mannosidase 2

Gene

alpha-Man-IIa

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity).By similarity

Catalytic activityi

Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153ZincBy similarity1
Metal bindingi155ZincBy similarity1
Active sitei267NucleophileBy similarity1
Metal bindingi267ZincBy similarity1
Metal bindingi534ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.2.1.114 1994
ReactomeiR-DME-975578 Reactions specific to the complex N-glycan synthesis pathway
SABIO-RKiQ24451
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH38 Glycoside Hydrolase Family 38

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2 (EC:3.2.1.1141 Publication)
Alternative name(s):
Golgi alpha-mannosidase II1 Publication
Short name:
AMan II
Short name:
Man II
Golgi alpha-mannosidase IIaImported
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:alpha-Man-IIaImported
Synonyms:GmII1 Publication
ORF Names:CG18802Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0011740 alpha-Man-IIa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 1108LumenalSequence analysisAdd BLAST1078

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002069061 – 1108Alpha-mannosidase 2Add BLAST1108

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ24451
PRIDEiQ24451

Expressioni

Inductioni

Inhibited by swainsonine and by copper sulfate.1 Publication

Gene expression databases

BgeeiFBgn0011740 Expressed in 32 organ(s), highest expression level in wing disc (Drosophila)
ExpressionAtlasiQ24451 baseline and differential
GenevisibleiQ24451 DM

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi66291, 3 interactors
DIPiDIP-22953N
IntActiQ24451, 5 interactors
STRINGi7227.FBpp0081509

Chemistry databases

BindingDBiQ24451

Structurei

Secondary structure

11108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ24451
SMRiQ24451
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ24451

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1959 Eukaryota
ENOG410XQMZ LUCA
GeneTreeiENSGT00510000046304
InParanoidiQ24451
KOiK01231
OMAiWRQNWDL
OrthoDBiEOG091G00YH
PhylomeDBiQ24451

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
2.60.40.1180, 2 hits
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
IPR013780 Glyco_hydro_b
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q24451-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRIRRRFAL VICSGCLLVF LSLYIILNFA APAATQIKPN YENIENKLHE
60 70 80 90 100
LENGLQEHGE EMRNLRARLA ETSNRDDPIR PPLKVARSPR PGQCQDVVQD
110 120 130 140 150
VPNVDVQMLE LYDRMSFKDI DGGVWKQGWN IKYDPLKYNA HHKLKVFVVP
160 170 180 190 200
HSHNDPGWIQ TFEEYYQHDT KHILSNALRH LHDNPEMKFI WAEISYFARF
210 220 230 240 250
YHDLGENKKL QMKSIVKNGQ LEFVTGGWVM PDEANSHWRN VLLQLTEGQT
260 270 280 290 300
WLKQFMNVTP TASWAIDPFG HSPTMPYILQ KSGFKNMLIQ RTHYSVKKEL
310 320 330 340 350
AQQRQLEFLW RQIWDNKGDT ALFTHMMPFY SYDIPHTCGP DPKVCCQFDF
360 370 380 390 400
KRMGSFGLSC PWKVPPRTIS DQNVAARSDL LVDQWKKKAE LYRTNVLLIP
410 420 430 440 450
LGDDFRFKQN TEWDVQRVNY ERLFEHINSQ AHFNVQAQFG TLQEYFDAVH
460 470 480 490 500
QAERAGQAEF PTLSGDFFTY ADRSDNYWSG YYTSRPYHKR MDRVLMHYVR
510 520 530 540 550
AAEMLSAWHS WDGMARIEER LEQARRELSL FQHHDGITGT AKTHVVVDYE
560 570 580 590 600
QRMQEALKAC QMVMQQSVYR LLTKPSIYSP DFSFSYFTLD DSRWPGSGVE
610 620 630 640 650
DSRTTIILGE DILPSKHVVM HNTLPHWREQ LVDFYVSSPF VSVTDLANNP
660 670 680 690 700
VEAQVSPVWS WHHDTLTKTI HPQGSTTKYR IIFKARVPPM GLATYVLTIS
710 720 730 740 750
DSKPEHTSYA SNLLLRKNPT SLPLGQYPED VKFGDPREIS LRVGNGPTLA
760 770 780 790 800
FSEQGLLKSI QLTQDSPHVP VHFKFLKYGV RSHGDRSGAY LFLPNGPASP
810 820 830 840 850
VELGQPVVLV TKGKLESSVS VGLPSVVHQT IMRGGAPEIR NLVDIGSLDN
860 870 880 890 900
TEIVMRLETH IDSGDIFYTD LNGLQFIKRR RLDKLPLQAN YYPIPSGMFI
910 920 930 940 950
EDANTRLTLL TGQPLGGSSL ASGELEIMQD RRLASDDERG LGQGVLDNKP
960 970 980 990 1000
VLHIYRLVLE KVNNCVRPSE LHPAGYLTSA AHKASQSLLD PLDKFIFAEN
1010 1020 1030 1040 1050
EWIGAQGQFG GDHPSAREDL DVSVMRRLTK SSAKTQRVGY VLHRTNLMQC
1060 1070 1080 1090 1100
GTPEEHTQKL DVCHLLPNVA RCERTTLTFL QNLEHLDGMV APEVCPMETA

AYVSSHSS
Length:1,108
Mass (Da):126,721
Last modified:June 16, 2003 - v2
Checksum:i55E370EC8CAC6D4A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4KFG0A0A0B4KFG0_DROME
Alpha-mannosidase
alpha-Man-IIa alpha-Man-II, CG18474, CG18802, CG8139, dGMII alpha-Man-IIa alpha-Man-II, CG18474, CG18802, CG8139, dGMII, dGMIIb, DM-GII.1, Dmel\CG18802, GMII, GmII, Man, MAN-2, Man-II, CG18802, Dmel_CG18802
1,109Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71E → K in CAA54732 (PubMed:7890162).Curated1
Sequence conflicti305 – 306QL → HV in CAA10755 (PubMed:10504337).Curated2
Sequence conflicti397L → V in CAA10755 (PubMed:10504337).Curated1
Sequence conflicti970E → K in CAA54732 (PubMed:7890162).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77652 mRNA Translation: CAA54732.1
AJ132715 Genomic DNA Translation: CAA10755.1
AE014297 Genomic DNA Translation: AAF54375.1
AY119464 mRNA Translation: AAM50118.1
PIRiJC4037
RefSeqiNP_524291.2, NM_079567.3
UniGeneiDm.2459

Genome annotation databases

EnsemblMetazoaiFBtr0082031; FBpp0081509; FBgn0011740
GeneIDi41126
KEGGidme:Dmel_CG18802

Similar proteinsi

Entry informationi

Entry nameiMAN2_DROME
AccessioniPrimary (citable) accession number: Q24451
Secondary accession number(s): Q9TYG5, Q9VHD8, Q9VHD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 16, 2003
Last modified: September 12, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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