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UniProtKB - Q24323 (SEM2A_DROME)
Protein
Semaphorin-2A
Gene
Sema2a
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Plays a role in growth cones guidance. Required for both proper adult behavior and survival. Can function in vivo as a selective target-derived signal that inhibits the formation of specific synaptic terminal arbors.
1 PublicationGO - Molecular functioni
- chemorepellent activity Source: GO_Central
- semaphorin receptor binding Source: GO_Central
GO - Biological processi
- adult behavior Source: FlyBase
- axon guidance Source: FlyBase
- dendrite guidance Source: FlyBase
- drinking behavior Source: FlyBase
- flight behavior Source: FlyBase
- negative chemotaxis Source: GO_Central
- negative regulation of axon extension involved in axon guidance Source: GO_Central
- olfactory bulb axon guidance Source: FlyBase
- positive regulation of cell migration Source: GO_Central
- semaphorin-plexin signaling pathway Source: GO_Central
- sensory neuron axon guidance Source: FlyBase
- synaptic target inhibition Source: FlyBase
- visual behavior Source: FlyBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-DME-399954, Sema3A PAK dependent Axon repulsion R-DME-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-DME-399956, CRMPs in Sema3A signaling R-DME-416572, Sema4D induced cell migration and growth-cone collapse R-DME-416700, Other semaphorin interactions |
SignaLinki | Q24323 |
Names & Taxonomyi
Protein namesi | Recommended name: Semaphorin-2AImportedAlternative name(s): Semaphorin-II1 Publication Short name: Sema II1 Publication |
Gene namesi | ORF Names:CG4700Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011260, Sema2a |
VEuPathDBi | VectorBase:FBgn0011260 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Cytosol
- cytosol Source: FlyBase
Extracellular region or secreted
- extracellular region Source: FlyBase
- extracellular space Source: GO_Central
Other locations
- endomembrane system Source: FlyBase
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000032301 | 26 – 724 | Semaphorin-2AAdd BLAST | 699 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 95 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 118 ↔ 129 | By similarity | ||
Glycosylationi | 163 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 190 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 291 ↔ 399 | By similarity | ||
Glycosylationi | 314 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 315 ↔ 358 | By similarity | ||
Glycosylationi | 401 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 525 ↔ 541 | By similarity | ||
Disulfide bondi | 535 ↔ 550 | By similarity | ||
Glycosylationi | 563 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 590 ↔ 647 | By similarity | ||
Glycosylationi | 658 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 670 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 708 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q24323 |
PRIDEi | Q24323 |
PTM databases
GlyGeni | Q24323, 10 sites |
iPTMneti | Q24323 |
Expressioni
Tissue specificityi
Transiently expressed by a single large muscle during motoneuron outgrowth and synapse formation.1 Publication
Developmental stagei
Expression begins around stage 10 in weak epidermal stripes. Nervous system expression is first detected around stage 15 and at stage 16 appears to remain restricted to a small subset of neurons. Beginning around stage 14, it is also expressed at a very high level in a single ventral thoracic muscle fiber in segment T3. It is also expressed in the embryonic gonads and in anterior sensory organs, including the maxillary complex.1 Publication
Gene expression databases
Bgeei | FBgn0011260, Expressed in wing disc (Drosophila) and 48 other tissues |
ExpressionAtlasi | Q24323, baseline and differential |
Genevisiblei | Q24323, DM |
Interactioni
Subunit structurei
Interacts with PlexB.
1 PublicationGO - Molecular functioni
- chemorepellent activity Source: GO_Central
- semaphorin receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 62559, 23 interactors |
IntActi | Q24323, 56 interactors |
STRINGi | 7227.FBpp0086237 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q24323 |
SMRi | Q24323 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 522 | SemaPROSITE-ProRule annotationAdd BLAST | 478 | |
Domaini | 552 – 663 | Ig-like C2-typeAdd BLAST | 112 |
Sequence similaritiesi
Belongs to the semaphorin family.Curated
Keywords - Domaini
Immunoglobulin domain, SignalPhylogenomic databases
eggNOGi | KOG3611, Eukaryota |
GeneTreei | ENSGT00940000172789 |
InParanoidi | Q24323 |
OMAi | GDYENTW |
PhylomeDBi | Q24323 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR001627, Semap_dom IPR036352, Semap_dom_sf IPR027231, Semaphorin IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR11036, PTHR11036, 1 hit |
Pfami | View protein in Pfam PF01403, Sema, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00630, Sema, 1 hit |
SUPFAMi | SSF101912, SSF101912, 1 hit SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS51004, SEMA, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform A (identifier: Q24323-1) [UniParc]FASTAAdd to basket
Also known as: B
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLLQLSPLL ALLLLLCSSV SETAADYENT WNFYYERPCC TGNDQGNNNY
60 70 80 90 100
GKHGADHVRE FNCGKLYYRT FHMNEDRDTL YVGAMDRVFR VNLQNISSSN
110 120 130 140 150
CNRDVINLEP TRDDVVSCVS KGKSQIFDCK NHVRVIQSMD QGDRLYVCGT
160 170 180 190 200
NAHNPKDYVI YANLTHLPRS EYVIGVGLGI AKCPYDPLDN STAIYVENGN
210 220 230 240 250
PGGLPGLYSG TNAEFTKADT VIFRTDLYNT SAKRLEYKFK RTLKYDSKWL
260 270 280 290 300
DKPNFVGSFD IGEYVYFFFR ETAVEYINCG KAVYSRIARV CKKDVGGKNL
310 320 330 340 350
LAHNWATYLK ARLNCSISGE FPFYFNEIQS VYQLPSDKSR FFATFTTSTN
360 370 380 390 400
GLIGSAVCSF HINEIQAAFN GKFKEQSSSN SAWLPVLNSR VPEPRPGTCV
410 420 430 440 450
NDTSNLPDTV LNFIRSHPLM DKAVNHEHNN PVYYKRDLVF TKLVVDKIRI
460 470 480 490 500
DILNQEYIVY YVGTNLGRIY KIVQYYRNGE SLSKLLDIFE VAPNEAIQVM
510 520 530 540 550
EISQTRKSLY IGTDHRIKQI DLAMCNRRYD NCFRCVRDPY CGWDKEANTC
560 570 580 590 600
RPYELDLLQD VANETSDICD SSVLKKKIVV TYGQSVHLGC FVKIPEVLKN
610 620 630 640 650
EQVTWYHHSK DKGRYEIRYS PTKYIETTER GLVVVSVNEA DGGRYDCHLG
660 670 680 690 700
GSLLCSYNIT VDAHRCTPPN KSNDYQKIYS DWCHEFEKYK TAMKSWEKKQ
710 720
AQCSTRQNFS SNQHPNEIFR KPNV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 105 | V → A in AAC37187 (PubMed:8269517).Curated | 1 | |
Sequence conflicti | 110 | P → S in AAR99105 (Ref. 5) Curated | 1 | |
Sequence conflicti | 701 | A → G in AAC37187 (PubMed:8269517).Curated | 1 | |
Sequence conflicti | 711 | S → C in AAC37187 (PubMed:8269517).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016075 | 1 – 55 | MSLLQ…GKHGA → MNGIHWLLFGSCLMLISQIE AVTEELSP in isoform C. CuratedAdd BLAST | 55 | |
Alternative sequenceiVSP_016076 | 103 – 125 | Missing in isoform D. CuratedAdd BLAST | 23 | |
Alternative sequenceiVSP_016077 | 110 – 127 | Missing in isoform E. 1 PublicationAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26083 mRNA Translation: AAC37187.1 AE013599 Genomic DNA Translation: AAF57989.1 AE013599 Genomic DNA Translation: AAM68500.1 AE013599 Genomic DNA Translation: AAS64837.1 AY095026 mRNA Translation: AAM11354.1 AY128437 mRNA Translation: AAM75030.1 BT011447 mRNA Translation: AAR99105.1 |
PIRi | C49423 |
RefSeqi | NP_477507.1, NM_058159.5 [Q24323-1] NP_599130.1, NM_134303.4 [Q24323-1] NP_725586.1, NM_166178.3 [Q24323-2] NP_995856.2, NM_206134.2 [Q24323-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0087088; FBpp0086236; FBgn0011260 [Q24323-2] FBtr0087089; FBpp0086237; FBgn0011260 [Q24323-1] FBtr0087090; FBpp0086238; FBgn0011260 [Q24323-1] FBtr0345339; FBpp0311494; FBgn0011260 [Q24323-1] |
GeneIDi | 36846 |
KEGGi | dme:Dmel_CG4700 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26083 mRNA Translation: AAC37187.1 AE013599 Genomic DNA Translation: AAF57989.1 AE013599 Genomic DNA Translation: AAM68500.1 AE013599 Genomic DNA Translation: AAS64837.1 AY095026 mRNA Translation: AAM11354.1 AY128437 mRNA Translation: AAM75030.1 BT011447 mRNA Translation: AAR99105.1 |
PIRi | C49423 |
RefSeqi | NP_477507.1, NM_058159.5 [Q24323-1] NP_599130.1, NM_134303.4 [Q24323-1] NP_725586.1, NM_166178.3 [Q24323-2] NP_995856.2, NM_206134.2 [Q24323-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6QP7 | X-ray | 1.96 | A/B | 27-671 | [»] | |
AlphaFoldDBi | Q24323 | |||||
SMRi | Q24323 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 62559, 23 interactors |
IntActi | Q24323, 56 interactors |
STRINGi | 7227.FBpp0086237 |
PTM databases
GlyGeni | Q24323, 10 sites |
iPTMneti | Q24323 |
Proteomic databases
PaxDbi | Q24323 |
PRIDEi | Q24323 |
Protocols and materials databases
DNASUi | 36846 |
Genome annotation databases
EnsemblMetazoai | FBtr0087088; FBpp0086236; FBgn0011260 [Q24323-2] FBtr0087089; FBpp0086237; FBgn0011260 [Q24323-1] FBtr0087090; FBpp0086238; FBgn0011260 [Q24323-1] FBtr0345339; FBpp0311494; FBgn0011260 [Q24323-1] |
GeneIDi | 36846 |
KEGGi | dme:Dmel_CG4700 |
Organism-specific databases
CTDi | 36846 |
FlyBasei | FBgn0011260, Sema2a |
VEuPathDBi | VectorBase:FBgn0011260 |
Phylogenomic databases
eggNOGi | KOG3611, Eukaryota |
GeneTreei | ENSGT00940000172789 |
InParanoidi | Q24323 |
OMAi | GDYENTW |
PhylomeDBi | Q24323 |
Enzyme and pathway databases
Reactomei | R-DME-399954, Sema3A PAK dependent Axon repulsion R-DME-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-DME-399956, CRMPs in Sema3A signaling R-DME-416572, Sema4D induced cell migration and growth-cone collapse R-DME-416700, Other semaphorin interactions |
SignaLinki | Q24323 |
Miscellaneous databases
BioGRID-ORCSi | 36846, 0 hits in 3 CRISPR screens |
ChiTaRSi | Sema-2a, fly |
GenomeRNAii | 36846 |
PROi | PR:Q24323 |
Gene expression databases
Bgeei | FBgn0011260, Expressed in wing disc (Drosophila) and 48 other tissues |
ExpressionAtlasi | Q24323, baseline and differential |
Genevisiblei | Q24323, DM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR001627, Semap_dom IPR036352, Semap_dom_sf IPR027231, Semaphorin IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR11036, PTHR11036, 1 hit |
Pfami | View protein in Pfam PF01403, Sema, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00630, Sema, 1 hit |
SUPFAMi | SSF101912, SSF101912, 1 hit SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS51004, SEMA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SEM2A_DROME | |
Accessioni | Q24323Primary (citable) accession number: Q24323 Secondary accession number(s): Q0E954 Q9V7Q7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 8, 2005 | |
Last modified: | May 25, 2022 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families