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Protein

Cullin homolog 1

Gene

Cul1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of multiple SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. During early metamorphosis, part of the SCF-slmb complex that negatively regulates the InR/PI3K/TOR pathway to activate the pruning of unnecessary larval ddaC dendrites and mushroom body axons (PubMed:24068890). The SCF-slmb complex also regulates asymmetrical division of neuroblasts and inhibits ectopic neuroblast formation partly through SAK and Akt1 (PubMed:24413555). Also part of an SCF complex required for caspase activation during sperm differentiation (PubMed:20392747). Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in the regulation of zip and ck function in scolopidial apical attachment (PubMed:27331610). May function by acting in a Ubr3-mediated pathway that negatively regulates the ubiquitination of zip, consequently affecting its interaction with ck (PubMed:27331610).4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: FlyBase

GO - Biological processi

  • negative regulation of apoptotic process Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: FlyBase
  • negative regulation of insulin receptor signaling pathway Source: FlyBase
  • negative regulation of phosphatidylinositol 3-kinase signaling Source: FlyBase
  • negative regulation of TOR signaling Source: FlyBase
  • positive regulation of neuron remodeling Source: FlyBase
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein catabolic process Source: FlyBase
  • protein ubiquitination Source: FlyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-68949 Orc1 removal from chromatin
R-DME-69231 Cyclin D associated events in G1
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8951664 Neddylation
R-DME-9020702 Interleukin-1 signaling
R-DME-917937 Iron uptake and transport
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ24311
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin homolog 1
Alternative name(s):
Lin-19 homolog protein
Gene namesi
Name:Cul1
Synonyms:cul-1, lin19
ORF Names:CG1877
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015509 Cul1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Severe dendrite pruning defects in ddaC neurons at 16 hours after puparium formation (APF) and axon pruning defects in mushroom body gamma neurons at 24 hours APF. RNAi-mediated knockdown results in a significant increase in Akt1 protein levels and activity in ddaC somas (PubMed:24068890). In the larval brain there is a large increase in the number of neuroblasts, 40% of which show a spindle misorientation at metaphase (PubMed:24413555). RNAi-mediated knockdown results in apical detachment of scolopidial cells in Johnston's organ (PubMed:27331610).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197861 – 774Cullin homolog 1Add BLAST774

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki718Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated (PubMed:12183637, PubMed:18493598). Deneddylated via its interaction with the COP9 signalosome (CSN) complex (PubMed:12183637).2 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ24311
PRIDEiQ24311

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiFBgn0015509
ExpressionAtlasiQ24311 baseline and differential
GenevisibleiQ24311 DM

Interactioni

Subunit structurei

Component of SCF E3 ubiquitin-protein ligase complexes consisting of Skpa, Cul1, Roc1a and an F-box protein. In larval neuroblast self renewal and asymmetric division, as well as ddaC dendrite and mushroom body axon pruning, the complex contains the F-box protein slmb (SCF-slmb) (PubMed:24068890, PubMed:24413555). In caspase activation during sperm differentiation, the complex contains the F-box protein ntc (PubMed:20392747). Interacts directly with Roc1a (PubMed:11500045). Interacts with Fsn (PubMed:20123973). Interacts with Dlish (PubMed:27692068). Interacts with Cad99C (via the cytoplasmic domain) (PubMed:27331610). Interacts with Sans (PubMed:27331610).7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: FlyBase

Protein-protein interaction databases

BioGridi61606, 38 interactors
DIPiDIP-19461N
IntActiQ24311, 8 interactors
MINTiQ24311
STRINGi7227.FBpp0087921

Structurei

3D structure databases

ProteinModelPortaliQ24311
SMRiQ24311
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166 Eukaryota
COG5647 LUCA
GeneTreeiENSGT00760000119212
HOGENOMiHOG000021491
InParanoidiQ24311
KOiK03347
OMAiTTHKHIE
OrthoDBiEOG091G02WR
PhylomeDBiQ24311

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q24311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSGNSQTT QKLVNLDDIW SELVEGIMQV FEHEKSLTRS QYMRFYTHVY
60 70 80 90 100
DYCTSVSAAP SGRSSGKTGG AQLVGKKLYD RLEQFLKSYL SELLTKFKAI
110 120 130 140 150
SGEEVLLSRY TKQWKSYQFS STVLDGICNY LNRNWVKREC EEGQKGIYKI
160 170 180 190 200
YRLALVAWKG HLFQVLNEPV TKAVLKSIEE ERQGKLINRS LVRDVIECYV
210 220 230 240 250
ELSFNEEDTD AEQQKLSVYK QNFENKFIAD TSAFYEKESD AFLSTNTVTE
260 270 280 290 300
YLKHVENRLE EETQRVRGFN SKNGLSYLHE TTADVLKSTC EEVLIEKHLK
310 320 330 340 350
IFHTEFQNLL NADRNDDLKR MYSLVALSSK NLTDLKSILE NHILHQGTEA
360 370 380 390 400
IAKCCTTDAA NDPKTYVQTI LDVHKKYNAL VLTAFNNDNG FVAALDKACG
410 420 430 440 450
KFINSNVVTI ANSASKSPEL LAKYCDLLLK KSSKNPEDKE LEDNLNQVMV
460 470 480 490 500
VFKYIEDKDV FQKYYSKMLA KRLVNHTSAS DDAEAMMISK LKQTCGYEYT
510 520 530 540 550
VKLQRMFQDI GVSKDLNSYF KQYLAEKNLT MEIDFGIEVL SSGSWPFQLS
560 570 580 590 600
NNFLLPSELE RSVRQFNEFY AARHSGRKLN WLYQMCKGEL IMNVNRNNSS
610 620 630 640 650
TYTLQASTFQ MSVLLQFNDQ LSFTVQQLQD NTQTQQENLI QVLQILLKAK
660 670 680 690 700
VLTSSDNENS LTPESTVELF LDYKNKKRRI NINQPLKTEL KVEQETVHKH
710 720 730 740 750
IEEDRKLLIQ AAIVRIMKMR KRLNHTNLIS EVLNQLSTRF KPKVPVIKKC
760 770
IDILIEKEYL ERMEGHKDTY SYLA
Length:774
Mass (Da):89,511
Last modified:November 2, 2001 - v2
Checksum:i1AF0D035DE85363C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266Missing in AAA85085 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41642 mRNA Translation: AAA85085.1
AF136343 mRNA Translation: AAD33676.1
AE013599 Genomic DNA Translation: AAF59174.1
AE013599 Genomic DNA Translation: AAM68871.1
AE013599 Genomic DNA Translation: AAM68872.1
BT010290 mRNA Translation: AAQ23608.1
RefSeqiNP_523655.1, NM_078931.4
NP_724621.1, NM_165569.2
NP_724622.1, NM_165570.3
NP_724623.1, NM_165571.2
UniGeneiDm.7926

Genome annotation databases

EnsemblMetazoaiFBtr0088845; FBpp0087921; FBgn0015509
FBtr0088846; FBpp0087922; FBgn0015509
FBtr0088847; FBpp0087923; FBgn0015509
FBtr0088848; FBpp0087924; FBgn0015509
GeneIDi35742
KEGGidme:Dmel_CG1877
UCSCiCG1877-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiCUL1_DROME
AccessioniPrimary (citable) accession number: Q24311
Secondary accession number(s): A4UZ78, Q0E9G0, Q9V312
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2001
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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