UniProtKB - Q24306 (DIAP1_DROME)
Death-associated inhibitor of apoptosis 1
Diap1
Functioni
Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling.
6 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.3 Publications EC:2.3.2.27
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 263 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 266 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 283 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 290 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 391 – 426 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 |
GO - Molecular functioni
- caspase binding Source: FlyBase
- cysteine-type endopeptidase inhibitor activity Source: FlyBase
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: FlyBase
- NEDD8 ligase activity Source: FlyBase
- ubiquitin conjugating enzyme binding Source: FlyBase
- ubiquitin-like protein conjugating enzyme binding Source: FlyBase
- ubiquitin protein ligase activity Source: FlyBase
- ubiquitin protein ligase binding Source: FlyBase
- ubiquitin-protein transferase activity Source: UniProtKB
- ubiquitin-specific protease binding Source: FlyBase
- zinc ion binding Source: FlyBase
GO - Biological processi
- antennal morphogenesis Source: FlyBase
- apoptotic process Source: FlyBase
- border follicle cell migration Source: FlyBase
- chaeta development Source: FlyBase
- inhibition of cysteine-type endopeptidase activity Source: FlyBase
- inhibition of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
- negative regulation of apoptotic process Source: FlyBase
- negative regulation of compound eye retinal cell programmed cell death Source: FlyBase
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: FlyBase
- negative regulation of JNK cascade Source: FlyBase
- positive regulation of border follicle cell migration Source: FlyBase
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- positive regulation of protein ubiquitination Source: FlyBase
- protein autoubiquitination Source: FlyBase
- protein K48-linked ubiquitination Source: FlyBase
- protein neddylation Source: FlyBase
- protein polyubiquitination Source: FlyBase
- regulation of cell cycle Source: GO_Central
- sensory organ precursor cell division Source: FlyBase
- spermatid nucleus differentiation Source: FlyBase
- spermatogenesis Source: FlyBase
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Apoptosis, Ubl conjugation pathway, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-111465, Apoptotic cleavage of cellular proteins R-DME-111469, SMAC, XIAP-regulated apoptotic response R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-390150, DS ligand bound to FT receptor R-DME-5675482, Regulation of necroptotic cell death R-DME-8948747, Regulation of PTEN localization R-DME-8948751, Regulation of PTEN stability and activity |
SignaLinki | Q24306 |
Protein family/group databases
MEROPSi | I32.009 |
Names & Taxonomyi
Protein namesi | Recommended name: Death-associated inhibitor of apoptosis 1 (EC:2.3.2.273 Publications)Alternative name(s): Apoptosis 1 inhibitor E3 ubiquitin-protein ligase th Inhibitor of apoptosis 1 Protein thread RING-type E3 ubiquitin transferase Diap1Curated |
Gene namesi | Name:Diap1 Synonyms:Iap1, th ORF Names:CG12284 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0260635, Diap1 |
VEuPathDBi | VectorBase:FBgn0260635 |
Subcellular locationi
Cytoskeleton
- spindle microtubule Source: GO_Central
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- perinuclear region of cytoplasm Source: FlyBase
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 162 | I → D: Destroys cleavage site. 1 Publication | 1 | |
Mutagenesisi | 165 | I → D: Destroys cleavage site. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122367 | 1 – 438 | Death-associated inhibitor of apoptosis 1Add BLAST | 438 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 162 – 163 | Cleavage; by HtrA2 | 2 | |
Sitei | 165 – 166 | Cleavage; by HtrA2 | 2 |
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q24306 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0260635, Expressed in eye disc (Drosophila) and 76 other tissues |
ExpressionAtlasi | Q24306, baseline and differential |
Genevisiblei | Q24306, DM |
Interactioni
Subunit structurei
Interacts (via BIR 2 domain) with Dronc (via residues 114-125). Rpr, hid and grim can outcompete Dronc for binding Diap1 therefore removing Diap1-mediated ubiquitination.
Interacts (via BIR 2 domain) with HtrA2; this displaces any bound Dronc.
Interacts with Strica (PubMed:11550090). The N-terminally cleaved form interacts with Ubr3 (via UBR-type zinc finger) (PubMed:25146930, PubMed:26383956); the interaction promotes the recruitment and uniquitination of substrate capases such as Dronc (PubMed:25146930).
7 PublicationsBinary interactionsi
Q24306
With | #Exp. | IntAct |
---|---|---|
chic [P25843] | 2 | EBI-456419,EBI-156199 |
Dronc [Q9XYF4] | 9 | EBI-456419,EBI-108311 |
hid [Q24106] | 8 | EBI-456419,EBI-135509 |
IKKepsilon [Q86PE7] | 3 | EBI-456419,EBI-987197 |
rpr [Q24475] | 6 | EBI-456419,EBI-106786 |
GO - Molecular functioni
- caspase binding Source: FlyBase
- ubiquitin conjugating enzyme binding Source: FlyBase
- ubiquitin-like protein conjugating enzyme binding Source: FlyBase
- ubiquitin protein ligase binding Source: FlyBase
- ubiquitin-specific protease binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 65064, 116 interactors |
IntActi | Q24306, 10 interactors |
MINTi | Q24306 |
STRINGi | 7227.FBpp0305795 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q24306 |
SMRi | Q24306 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q24306 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 44 – 110 | BIR 1Add BLAST | 67 | |
Repeati | 226 – 293 | BIR 2Add BLAST | 68 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 213 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 322 – 346 | DisorderedSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 391 – 426 | RING-typePROSITE-ProRule annotationAdd BLAST | 36 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1101, Eukaryota |
HOGENOMi | CLU_016347_1_0_1 |
InParanoidi | Q24306 |
OMAi | VQANYPH |
OrthoDBi | 1340284at2759 |
PhylomeDBi | Q24306 |
Family and domain databases
CDDi | cd00022, BIR, 2 hits |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001370, BIR_rpt IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF00653, BIR, 2 hits |
SMARTi | View protein in SMART SM00238, BIR, 2 hits SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS01282, BIR_REPEAT_1, 2 hits PS50143, BIR_REPEAT_2, 2 hits PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MASVVADLPS YGPIAFDQVD NNTNATQLFK NNINKTRMND LNREETRLKT
60 70 80 90 100
FTDWPLDWLD KRQLAQTGMY FTHAGDKVKC FFCGVEIGCW EQEDQPVPEH
110 120 130 140 150
QRWSPNCPLL RRRTTNNVPI NAEALDRILP PISYDICGAN DSTLEMREHA
160 170 180 190 200
YAEGVIPMSQ LIQSIGMNAV NAAGSVTGTA APQPRVTVAT HASTATQATG
210 220 230 240 250
DVQPETCRPS AASGNYFPQY PEYAIETARL RTFEAWPRNL KQKPHQLAEA
260 270 280 290 300
GFFYTGVGDR VRCFSCGGGL MDWNDNDEPW EQHALWLSQC RFVKLMKGQL
310 320 330 340 350
YIDTVAAKPV LAEEKEESSS IGGVAVASTQ ASEEEQQTSL SSEEAVSGDV
360 370 380 390 400
APSVAPTAAT RIFNKIVEAT AVATPSTNSS GSTSIPEEKL CKICYGAEYN
410 420 430
TAFLPCGHVV ACAKCASSVT KCPLCRKPFT DVMRVYFS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PI74 | M9PI74_DROME | Death-associated inhibitor of apopt... | Diap1 01, 03, 0736, 1065, D-IAP1 | 438 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 319 | S → T in AAC41609 (PubMed:8548811).Curated | 1 | |
Sequence conflicti | 324 – 325 | VA → DT in AAC41609 (PubMed:8548811).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L49440 mRNA Translation: AAC41609.1 AE014296 Genomic DNA Translation: AAF49548.1 AE014296 Genomic DNA Translation: AAG22319.1 AE014296 Genomic DNA Translation: AAN11757.1 BT031197 mRNA Translation: ABY20438.2 AY119524 mRNA Translation: AAM50178.1 Sequence problems. |
RefSeqi | NP_001261916.1, NM_001274987.1 NP_001261917.1, NM_001274988.1 NP_001261918.1, NM_001274989.1 NP_524101.2, NM_079377.3 NP_730097.1, NM_168644.2 NP_730098.1, NM_168645.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0075499; FBpp0075254; FBgn0260635 FBtr0075500; FBpp0075255; FBgn0260635 FBtr0075501; FBpp0075256; FBgn0260635 FBtr0333617; FBpp0305793; FBgn0260635 FBtr0333618; FBpp0305794; FBgn0260635 FBtr0333619; FBpp0305795; FBgn0260635 |
GeneIDi | 39753 |
KEGGi | dme:Dmel_CG12284 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L49440 mRNA Translation: AAC41609.1 AE014296 Genomic DNA Translation: AAF49548.1 AE014296 Genomic DNA Translation: AAG22319.1 AE014296 Genomic DNA Translation: AAN11757.1 BT031197 mRNA Translation: ABY20438.2 AY119524 mRNA Translation: AAM50178.1 Sequence problems. |
RefSeqi | NP_001261916.1, NM_001274987.1 NP_001261917.1, NM_001274988.1 NP_001261918.1, NM_001274989.1 NP_524101.2, NM_079377.3 NP_730097.1, NM_168644.2 NP_730098.1, NM_168645.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JD4 | X-ray | 2.70 | A/B | 201-323 | [»] | |
1JD5 | X-ray | 1.90 | A | 201-323 | [»] | |
1JD6 | X-ray | 2.70 | A | 201-323 | [»] | |
1Q4Q | X-ray | 2.10 | A/B/C/D/E/F/G/H/I/J | 201-323 | [»] | |
1SDZ | X-ray | 1.78 | A | 30-145 | [»] | |
1SE0 | X-ray | 1.75 | A | 30-145 | [»] | |
3SIP | X-ray | 3.50 | E/F | 31-145 | [»] | |
3SIQ | X-ray | 2.40 | A/B/C/D/E/F | 1-135 | [»] | |
AlphaFoldDBi | Q24306 | |||||
SMRi | Q24306 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 65064, 116 interactors |
IntActi | Q24306, 10 interactors |
MINTi | Q24306 |
STRINGi | 7227.FBpp0305795 |
Protein family/group databases
MEROPSi | I32.009 |
Proteomic databases
PaxDbi | Q24306 |
Genome annotation databases
EnsemblMetazoai | FBtr0075499; FBpp0075254; FBgn0260635 FBtr0075500; FBpp0075255; FBgn0260635 FBtr0075501; FBpp0075256; FBgn0260635 FBtr0333617; FBpp0305793; FBgn0260635 FBtr0333618; FBpp0305794; FBgn0260635 FBtr0333619; FBpp0305795; FBgn0260635 |
GeneIDi | 39753 |
KEGGi | dme:Dmel_CG12284 |
Organism-specific databases
CTDi | 39753 |
FlyBasei | FBgn0260635, Diap1 |
VEuPathDBi | VectorBase:FBgn0260635 |
Phylogenomic databases
eggNOGi | KOG1101, Eukaryota |
HOGENOMi | CLU_016347_1_0_1 |
InParanoidi | Q24306 |
OMAi | VQANYPH |
OrthoDBi | 1340284at2759 |
PhylomeDBi | Q24306 |
Enzyme and pathway databases
Reactomei | R-DME-111465, Apoptotic cleavage of cellular proteins R-DME-111469, SMAC, XIAP-regulated apoptotic response R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-390150, DS ligand bound to FT receptor R-DME-5675482, Regulation of necroptotic cell death R-DME-8948747, Regulation of PTEN localization R-DME-8948751, Regulation of PTEN stability and activity |
SignaLinki | Q24306 |
Miscellaneous databases
BioGRID-ORCSi | 39753, 1 hit in 3 CRISPR screens |
EvolutionaryTracei | Q24306 |
GenomeRNAii | 39753 |
PROi | PR:Q24306 |
Gene expression databases
Bgeei | FBgn0260635, Expressed in eye disc (Drosophila) and 76 other tissues |
ExpressionAtlasi | Q24306, baseline and differential |
Genevisiblei | Q24306, DM |
Family and domain databases
CDDi | cd00022, BIR, 2 hits |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001370, BIR_rpt IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF00653, BIR, 2 hits |
SMARTi | View protein in SMART SM00238, BIR, 2 hits SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS01282, BIR_REPEAT_1, 2 hits PS50143, BIR_REPEAT_2, 2 hits PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DIAP1_DROME | |
Accessioni | Q24306Primary (citable) accession number: Q24306 Secondary accession number(s): A4V1Z9 Q9VUX5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 23, 2004 | |
Last modified: | May 25, 2022 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families