UniProtKB - Q24256 (BARH2_DROME)
Protein
Homeobox protein B-H2
Gene
B-H2
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
B-H1 and B-H2 are regulated by members of the wg signaling pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally required in R1 and R6 receptor cells and primary pigment cells for normal eye development. Coexpression is also required for the fate determination of external sensory organs, formation of notal microchaetae, formation of presutural macrochaetae, antennal development and for distal leg morphogenesis; segmentation and specification of tarsal segments 3-5.4 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 380 – 439 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: FlyBase
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- chaeta morphogenesis Source: UniProtKB
- compound eye photoreceptor cell differentiation Source: FlyBase
- eye-antennal disc morphogenesis Source: UniProtKB
- eye pigment granule organization Source: UniProtKB
- leg disc proximal/distal pattern formation Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- sensory organ boundary specification Source: UniProtKB
- visual perception Source: UniProtKB-KW
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Sensory transduction, Vision, Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein B-H2Alternative name(s): Homeobox protein BarH2 |
Gene namesi | Name:B-H2 Synonyms:BarH2 ORF Names:CG5488 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004854, B-H2 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048833 | 1 – 645 | Homeobox protein B-H2Add BLAST | 645 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 593 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 595 | Phosphoserine1 Publication | 1 | |
Modified residuei | 602 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q24256 |
PTM databases
iPTMneti | Q24256 |
Expressioni
Tissue specificityi
B-H1 and B-H2 are abundant in the eye-antenna imaginal disk. Expressed in R1 and R6 cells throughout larval stage until 30 hours after puparium formation, at which time expression is seen in the anterior and posterior primary pigment cells. Coexpressed in embryonic glial cells, neurons of the CNS and PNS, most latitudinal anterior cells of the developing notum and the central circular region of the leg and antennal imaginal disk throughout larval development.4 Publications
Developmental stagei
Coexpressed at high levels with B-H1 in embryo and pupae and at low levels in larvae.1 Publication
Gene expression databases
Bgeei | FBgn0004854, Expressed in eye disc (Drosophila) and 42 other tissues |
ExpressionAtlasi | Q24256, baseline and differential |
Genevisiblei | Q24256, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 59051, 20 interactors |
IntActi | Q24256, 18 interactors |
STRINGi | 7227.FBpp0074203 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 85 | Ala-richAdd BLAST | 76 | |
Compositional biasi | 101 – 204 | Gln-richAdd BLAST | 104 | |
Compositional biasi | 107 – 256 | His-richAdd BLAST | 150 | |
Compositional biasi | 272 – 321 | Asn-richAdd BLAST | 50 | |
Compositional biasi | 283 – 377 | Ser-richAdd BLAST | 95 | |
Compositional biasi | 447 – 578 | Ala-richAdd BLAST | 132 | |
Compositional biasi | 607 – 644 | Glu-richAdd BLAST | 38 |
Sequence similaritiesi
Belongs to the Antp homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0488, Eukaryota |
GeneTreei | ENSGT00940000169370 |
HOGENOMi | CLU_393440_0_0_1 |
InParanoidi | Q24256 |
PhylomeDBi | Q24256 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q24256-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTMPPEMSA TTAAPVGSAP SATAHHPAAV GGGMPRPASP AVGSNTTTAT
60 70 80 90 100
ATTATRSRFM ITDILAGAAA ASAAAAAAAA ALAAASSGGG RGSPTDSERE
110 120 130 140 150
QSLVAQHHHH HQQQQQHHHH QQQQQQQQHQ QAALQQYIVQ QQQLLRFERE
160 170 180 190 200
REREREREHY RERHSPPGNN PYAHHPMPPH LLAHFPPAHY AVLQQQQQQQ
210 220 230 240 250
QQQQHPHPHH LQLERERLEA LHRHGHGLTG DPAQHLSHLS HLSHQQHHPH
260 270 280 290 300
LHHPMHDERS RSPLMLQQLG GNGGNNNNNN NNSSSASNNN NNNNNSASAN
310 320 330 340 350
SNIISGNSSS SNNNNGSGNG NMLLGGPGSS ISGDQASTID DSDSDDCGGK
360 370 380 390 400
DDDGDDSMKN GSSANGDSSS HLSLSLSKKQ RKARTAFTDH QLQTLEKSFE
410 420 430 440 450
RQKYLSVQDR MELANKLELS DCQVKTWYQN RRTKWKRQTA VGLELLAEAG
460 470 480 490 500
NYAAFQRLYG GATPYLSAWP YAAAAAAQSP HGATPSAIDI YYRQAAAAAA
510 520 530 540 550
MQKPSLPASY RMYQSSIPPG MSLPGMPAPP PPGAAPMLSG YYAAAAAAAA
560 570 580 590 600
SAGAQQQQQQ PPAASRSPAT SQSANSEADC ERTSSSSRQR LITPSPPLNP
610 620 630 640
GSPPHRERIN EEEDRERDEE RDIERERERE RERDEDDEEE LALEV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PHF0 | M9PHF0_DROME | BarH2, isoform B | B-H2 B, Bar, Bar-H2, bar-h2, BarH | 303 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 121 – 123 | Missing in AAB59218 (PubMed:1346120).Curated | 3 | |
Sequence conflicti | 229 | T → P in AAB59218 (PubMed:1346120).Curated | 1 | |
Sequence conflicti | 288 – 289 | Missing in AAB59218 (PubMed:1346120).Curated | 2 | |
Sequence conflicti | 375 | S → N in AAB59218 (PubMed:1346120).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M82884 mRNA Translation: AAA28383.2 M82887, M82885, M82886 Genomic DNA Translation: AAB59218.1 AE014298 Genomic DNA Translation: AAF48725.1 BT022144 mRNA Translation: AAY51539.1 |
PIRi | A41726 S27806 |
RefSeqi | NP_523386.1, NM_078662.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0074429; FBpp0074203; FBgn0004854 |
GeneIDi | 32723 |
KEGGi | dme:Dmel_CG5488 |
UCSCi | CG5488-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M82884 mRNA Translation: AAA28383.2 M82887, M82885, M82886 Genomic DNA Translation: AAB59218.1 AE014298 Genomic DNA Translation: AAF48725.1 BT022144 mRNA Translation: AAY51539.1 |
PIRi | A41726 S27806 |
RefSeqi | NP_523386.1, NM_078662.3 |
3D structure databases
SMRi | Q24256 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59051, 20 interactors |
IntActi | Q24256, 18 interactors |
STRINGi | 7227.FBpp0074203 |
PTM databases
iPTMneti | Q24256 |
Proteomic databases
PaxDbi | Q24256 |
Genome annotation databases
EnsemblMetazoai | FBtr0074429; FBpp0074203; FBgn0004854 |
GeneIDi | 32723 |
KEGGi | dme:Dmel_CG5488 |
UCSCi | CG5488-RA, d. melanogaster |
Organism-specific databases
CTDi | 32723 |
FlyBasei | FBgn0004854, B-H2 |
Phylogenomic databases
eggNOGi | KOG0488, Eukaryota |
GeneTreei | ENSGT00940000169370 |
HOGENOMi | CLU_393440_0_0_1 |
InParanoidi | Q24256 |
PhylomeDBi | Q24256 |
Miscellaneous databases
BioGRID-ORCSi | 32723, 0 hits in 3 CRISPR screens |
ChiTaRSi | B-H2, fly |
GenomeRNAii | 32723 |
PROi | PR:Q24256 |
Gene expression databases
Bgeei | FBgn0004854, Expressed in eye disc (Drosophila) and 42 other tissues |
ExpressionAtlasi | Q24256, baseline and differential |
Genevisiblei | Q24256, DM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BARH2_DROME | |
Accessioni | Q24256Primary (citable) accession number: Q24256 Secondary accession number(s): Q24257, Q4V712, Q9VX55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families