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Protein

Protein stoned-B

Gene

stnB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein involved in endocytic recycling of synaptic vesicles membranes. May act by mediating the retrieval of synaptotagmin protein Syt from the plasma membrane, thereby facilitating the internalization of multiple synaptic vesicles from the plasma membrane.3 Publications

Miscellaneous

StnA, which is involved in the same pathway, is derived from the same bicistronic transcript that encodes these two different proteins.

GO - Molecular functioni

  • scaffold protein binding Source: FlyBase

GO - Biological processi

  • chemical synaptic transmission Source: FlyBase
  • regulation of synaptic vesicle endocytosis Source: FlyBase
  • synaptic vesicle cycle Source: FlyBase
  • synaptic vesicle endocytosis Source: UniProtKB
  • synaptic vesicle transport Source: UniProtKB

Keywordsi

Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-DME-8856825 Cargo recognition for clathrin-mediated endocytosis
R-DME-8856828 Clathrin-mediated endocytosis
SignaLinkiQ24212

Names & Taxonomyi

Protein namesi
Recommended name:
Protein stoned-B
Short name:
Stn-B
Short name:
StonedB
Gene namesi
Name:stnB
ORF Names:CG12473
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0016975 stnB

Subcellular locationi

Keywords - Cellular componenti

Cell junction, Cytoplasm, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857351 – 1262Protein stoned-BAdd BLAST1262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei623Phosphoserine1 Publication1
Modified residuei626Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24212
PRIDEiQ24212

PTM databases

iPTMnetiQ24212

Expressioni

Developmental stagei

Present at synaptic connections both in the CNS and in neuromuscular junctions in the mature embryo (20-22h) and throughout larval development. In the third instar larva, it is expressed in all synaptic bouton types, including I, II and III boutons.1 Publication

Gene expression databases

BgeeiFBgn0016975
GenevisibleiQ24212 DM

Interactioni

Subunit structurei

Interacts with the second C2 domain of Syt.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt1P215215EBI-604879,EBI-484504

GO - Molecular functioni

  • scaffold protein binding Source: FlyBase

Protein-protein interaction databases

ELMiQ24212
IntActiQ24212, 4 interactors
STRINGi7227.FBpp0291230

Structurei

3D structure databases

ProteinModelPortaliQ24212
SMRiQ24212
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini728 – 902SHDPROSITE-ProRule annotationAdd BLAST175
Domaini906 – 1219MHDPROSITE-ProRule annotationAdd BLAST314

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni847 – 1108Interaction with SytAdd BLAST262

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 5NPF 13
Motifi19 – 21NPF 23
Motifi33 – 35NPF 33
Motifi43 – 45NPF 43
Motifi210 – 212NPF 53
Motifi493 – 495NPF 63
Motifi673 – 675NPF 73

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 295Pro-richAdd BLAST85

Domaini

The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, are known to interact with the EH domain.By similarity

Sequence similaritiesi

Belongs to the Stoned B family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2677 Eukaryota
ENOG410XQHF LUCA
InParanoidiQ24212
KOiK20067
OMAiPCEDVSI
OrthoDBiEOG091G0GC2
PhylomeDBiQ24212

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR028565 MHD
IPR012320 SHD_dom
IPR031232 Ston1
IPR017110 Stonin
PANTHERiPTHR45159 PTHR45159, 1 hit
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PIRSFiPIRSF037099 Stonin, 1 hit
SUPFAMiSSF49447 SSF49447, 2 hits
PROSITEiView protein in PROSITE
PS51072 MHD, 1 hit
PS51070 SHD, 1 hit

Sequencei

Sequence statusi: Complete.

Q24212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPFLMDED LDGCDAAANP FLMQSEPEPS SDNPFMAATV ASNPFAFGAD
60 70 80 90 100
DLELGAEPEA EATHDNDLDP AMSFFGTTIE AEDDTLSLKS GAEEEDEGKK
110 120 130 140 150
PPQSQPQLQS HAHPHPPPPR PLVPPQSTQD LISTVSSQLD ETSSELLGRI
160 170 180 190 200
PATRSPSPVS MRDLHSPSPT PDSGLADLLD VSVDSGSSAH TQGIEADLIS
210 220 230 240 250
GVAGGVRLDN PFAVPTAVPN IQAAVPLPAT PIKQPPRPPP PRPAPPRPAP
260 270 280 290 300
PGQAAPQRPP PPLAAVNPPP AAPEADDLLD MFGTTACKPA KPPPPKSKED
310 320 330 340 350
ILSLFEQPHV PLSQPASKPD LLHDDLDETI GEGEPPEQEE PDTEQSNEIS
360 370 380 390 400
SRDEPVFTSL LIRPDESTHD ITSQPQAATG LERQVNNMAA PSGTASTQRA
410 420 430 440 450
TTPDIEITTV EDLPRSDDED EPEAMQEPET ETKPQIEPDT EPEIVSEHSP
460 470 480 490 500
PTERLVTQAA LVDGELIAAE PEPEEMDTGL DFPLASSGQL SANPFASPDE
510 520 530 540 550
EEPNFAPMPA AVANIFAVND PDSQMETPKA PSHTANIFAS DPDEFDAFSA
560 570 580 590 600
KFDSVKKDNI SIMDGFGGSG AITPTGGDAW GGSAFGSTTI SANACGDTNS
610 620 630 640 650
ADDGFGNDDD DFYAMQAPAR ADSVESVDKD FSVVIRPMAE ETSGVAPQLA
660 670 680 690 700
PPPPPARGVN QAQTTSLPGL TVNPFEDVSG FPAPGLPPTD GTAIKRTDSQ
710 720 730 740 750
DTPQTPLYDE DVSQPLEEFP RLHYIGPGWE MQLRQPNKKK ITGQRFWKKI
760 770 780 790 800
VVRLVVQNDV PVVQLLNQAG DKQPFQELPL QPSYSVSEIG AQQYDQFGKI
810 820 830 840 850
FTMKLQYIFY KERPGVRPGQ VTKAERITNK LTKFAQYAIA GDYEGVKEFG
860 870 880 890 900
SDLKKLGLPV EHAPQSSQLF KIGSMNYEDM KQFSVCIEEA LFKIPALRER
910 920 930 940 950
ALTYKMEEVQ VTAVDEITVE QDFEGKILKQ IARVRLFFLA FLTGMPTIEL
960 970 980 990 1000
GVNDMWRQGK EVVGRHDIIP VATEEWIRLE AVEFHSVVNQ KEYERTRTIK
1010 1020 1030 1040 1050
FQPPDANYIE LLRFRVRPPK NRELPLQLKA TWCVSGNKVE LRADILVPGF
1060 1070 1080 1090 1100
TSRKLGQIPC EDVSVRFPIP ECWIYLFRVE KHFRYGSVKS AHRRTGKIKG
1110 1120 1130 1140 1150
IERILGAVDT LQESLIEVTS GQAKYEHHHR AIVWRCPRLP KEGQGAYTTH
1160 1170 1180 1190 1200
QLVCRMALTS YDQIPSELAP YAFVEFTMPA TQVSHTTVRS VSVQDSDGDE
1210 1220 1230 1240 1250
PPEKYVRYLA RHEYKVGIET THGESTNAYL AATRPIREEP PTTATKPTAS
1260
PVAPSDSDTD SN
Length:1,262
Mass (Da):137,781
Last modified:October 19, 2011 - v4
Checksum:i877E3EE6B964C23A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti657 – 658RG → VE in AAC16666 (PubMed:8844157).Curated2
Sequence conflicti1012L → V in AAC16666 (PubMed:8844157).Curated1

RNA editingi

Edited at position 1186.1 Publication
Partially edited.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1186T → A in RNA edited version. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54982 mRNA Translation: AAC16666.1
AE014298 Genomic DNA Translation: ABI31012.2
AE014298 Genomic DNA Translation: EAA46059.3
PIRiT13353
RefSeqiNP_001036318.2, NM_001042853.3
NP_001036319.2, NM_001042854.3
NP_001285539.1, NM_001298610.1

Genome annotation databases

EnsemblMetazoaiFBtr0302020; FBpp0291230; FBgn0016975
FBtr0302021; FBpp0291231; FBgn0016975
FBtr0344111; FBpp0310530; FBgn0016975
GeneIDi4379834
KEGGidme:Dmel_CG12473
UCSCiCG12473-RB d. melanogaster

Keywords - Coding sequence diversityi

RNA editing

Similar proteinsi

Entry informationi

Entry nameiSTNB_DROME
AccessioniPrimary (citable) accession number: Q24212
Secondary accession number(s): A4V4V9, Q7PLF4, Q9W5J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 19, 2011
Last modified: July 18, 2018
This is version 150 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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