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Protein

Peripheral plasma membrane protein CASK

Gene

CASK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May regulate transmembrane proteins that bind calcium, calmodulin, or nucleotides. Functionally modulates eag potassium channels; increases eag current and whole-cell conductance. Also regulates autophosphorylation of CaMKII.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei141By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.17 1994
ReactomeiR-DME-212676 Dopamine Neurotransmitter Release Cycle
R-DME-6794361 Neurexins and neuroligins
SignaLinkiQ24210

Protein family/group databases

TCDBi9.B.106.3.2 the pock size-determining protein (psdp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Peripheral plasma membrane protein CASK (EC:2.7.11.17)
Short name:
dCASK
Alternative name(s):
Calcium/calmodulin-dependent protein kinase
Short name:
CAKI
Short name:
Camguk
Gene namesi
Name:CASK
Synonyms:Caki, cmg
ORF Names:CG6703
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0013759 CASK

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increases synapse-specific, activity-dependent autophosphorylation of CaMKII Thr-287.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945711 – 898Peripheral plasma membrane protein CASKAdd BLAST898

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24210
PRIDEiQ24210

Expressioni

Tissue specificityi

During embryogenesis, larval and pupal life, found almost exclusively in the central nervous system. In the adult head found in the lamina, the neuropil of the medulla, lobula, lobula plate and in the central brain.1 Publication

Gene expression databases

BgeeiFBgn0013759 Expressed in 28 organ(s), highest expression level in head
ExpressionAtlasiQ24210 baseline and differential
GenevisibleiQ24210 DM

Interactioni

Subunit structurei

Interacts with eag. Interacts with CaMKII.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi67530, 14 interactors
DIPiDIP-19769N
IntActiQ24210, 7 interactors
STRINGi7227.FBpp0303394

Structurei

3D structure databases

ProteinModelPortaliQ24210
SMRiQ24210
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 278Protein kinasePROSITE-ProRule annotationAdd BLAST267
Domaini343 – 397L27 1PROSITE-ProRule annotationAdd BLAST55
Domaini402 – 461L27 2PROSITE-ProRule annotationAdd BLAST60
Domaini495 – 576PDZPROSITE-ProRule annotationAdd BLAST82
Domaini582 – 651SH3PROSITE-ProRule annotationAdd BLAST70
Domaini711 – 883Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST173

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni306 – 316Calmodulin-bindingAdd BLAST11

Sequence similaritiesi

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated
Belongs to the MAGUK family.Curated

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0033 Eukaryota
KOG0609 Eukaryota
COG0194 LUCA
GeneTreeiENSGT00760000118866
InParanoidiQ24210
KOiK06103
OrthoDBiEOG091G065I
PhylomeDBiQ24210

Family and domain databases

CDDicd12081 SH3_CASK, 1 hit
InterProiView protein in InterPro
IPR035473 CASK_SH3
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR020590 Guanylate_kinase_CS
IPR011009 Kinase-like_dom_sf
IPR014775 L27_C
IPR004172 L27_dom
IPR036892 L27_dom_sf
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000719 Prot_kinase_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF02828 L27, 2 hits
PF00595 PDZ, 1 hit
PF00069 Pkinase, 1 hit
PF07653 SH3_2, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00569 L27, 2 hits
SM00228 PDZ, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF101288 SSF101288, 2 hits
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 1 hit
SSF52540 SSF52540, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
PS51022 L27, 2 hits
PS50106 PDZ, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50002 SH3, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform B (identifier: Q24210-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEDEILFDD VYELCEVIGK GPFSIVRRCI HRESNQQFAV KIVDVAKFTA
60 70 80 90 100
SPGLSTADLK REATICHMLK HPHIVELLET YSSEGMLYMV FEFMEGSDLC
110 120 130 140 150
FEVVRRAVAG FVYSEAVACH YMRQILEALR YCHENDILHR DVRPACALLA
160 170 180 190 200
TVDNSAPVKL GGFGSAIQLP GTRETIETHG RVGCPHYMAP EVVTRRLYGK
210 220 230 240 250
GCDVWGAGVM LHVLLSGRLP FLGSGVRLQQ SVARGRLSFE APEWKSISAN
260 270 280 290 300
AKDLVMKMLA ANPHHRLSIT EVLDHPWIRD RDKLQRTHLA DTVEELKRYN
310 320 330 340 350
ARRKLKGAVQ AIAGGTNMDP LYATDADMPI TGATDEWADE EAGIEAVQRI
360 370 380 390 400
LDCLDDIYSL QDAHVDADVL RDMLRDNRLH QFLQLFDRIA ATVVTSNGRA
410 420 430 440 450
PAAEAVGRCR DVLEQLSSTS GGNSLGGKYA KEELMRLLAA PHMQALLHSH
460 470 480 490 500
DVVARDVYGE EALRVTPPPM VPYLNGDELD NVEGGELQHV TRVRLVQFQK
510 520 530 540 550
NTDEPMGITL KMTEDGRCIV ARIMHGGMIH RQATLHVGDE IREINGQPVQ
560 570 580 590 600
HQSVGQLQRM LREARGSVTF KIVPSYRSAP PPCEIFVRAQ FDYNPLDDEL
610 620 630 640 650
IPCAQAGISF QVGDILQIIS KDDHHWWQAR LDTVGGSAGL IPSPELQEWR
660 670 680 690 700
IACQTVDKTK QEQVNCSIFG RKKKQCRDKY LAKHNAIFDT LDVVTYEEVV
710 720 730 740 750
KVPVGDPNFQ RKTLVLLGAH GVGRRHIKNT LISKYPDKYA YPIPHTTRPA
760 770 780 790 800
KPEEENGRSY YFVSHDEMMA DIGANEYLEY GTHEDAMYGT KLDTIRRIHT
810 820 830 840 850
EGKMAILDVE PQALKILRTA EFTPYVVFIA APSLQNIADY DGSLERLAKE
860 870 880 890
SEMLRQLYGH FFDLTIVNND ISETIATLET AIDRVHTTPQ WVPVSWLY
Length:898
Mass (Da):100,911
Last modified:August 22, 2003 - v4
Checksum:i5B16014EC66E0E9B
GO
Isoform A (identifier: Q24210-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-317: Missing.
     318-443: MDPLYATDAD...LMRLLAAPHM → MCQHHGLTSQ...VPNGSICSGH
     584-584: E → ELFRIRPAPVL

Note: No experimental confirmation available.
Show »
Length:591
Mass (Da):65,197
Checksum:i645638C40343145C
GO
Isoform G (identifier: Q24210-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-317: Missing.
     318-443: MDPLYATDAD...LMRLLAAPHM → MCQHHGLTSQ...VPNGSICSGH
     584-584: E → ELFRIRPAPVL
     663-663: Q → QGEPGAGCSAHADGCDGSA

Note: No experimental confirmation available.
Show »
Length:609
Mass (Da):66,741
Checksum:i7F0A65E1DD31AD9D
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E1JIS7E1JIS7_DROME
CASK, isoform F
CASK CAKI, Caki, caki, CAMGUK, CamGUK
916Annotation score:
A0A0B4KH39A0A0B4KH39_DROME
CASK, isoform H
CASK CAKI, Caki, caki, CAMGUK, CamGUK
929Annotation score:
A8JR71A8JR71_DROME
CASK, isoform D
CASK CAKI, Caki, caki, Caki-RD, CAMGUK
591Annotation score:
A8JR72A8JR72_DROME
CASK, isoform E
CASK CAKI, Caki, caki, CAMGUK, CamGUK
469Annotation score:

Sequence cautioni

The sequence AAM11269 differs from that shown. Probable cloning artifact.Curated
The sequence AAO39536 differs from that shown. Reason: Frameshift at position 114.Curated
The sequence CAA63940 differs from that shown. Reason: Frameshift at positions 537 and 667.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → V in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti136D → E in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti463L → A in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti465 – 477VTPPP…YLNGD → HPAPDGALPQWR in AAC80169 (PubMed:9178262).CuratedAdd BLAST13
Sequence conflicti497Q → H in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti523I → L in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti632D → G in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti740A → V in AAC80169 (PubMed:9178262).Curated1
Sequence conflicti759S → N in AAO39554 (PubMed:12537569).Curated1
Sequence conflicti886 – 892HTTPQWV → DIPPRSGC in CAA63940 (PubMed:8617233).Curated7

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0080881 – 317Missing in isoform A and isoform G. 1 PublicationAdd BLAST317
Alternative sequenceiVSP_008089318 – 443MDPLY…AAPHM → MCQHHGLTSQSLMSGGSHIS LLGSSGSSGMSGSGVGSSGQ SVPQCPAAVAAADAAMMGSN AGGHCRSLSGLSSISIPPPP PALFNPCSSALSLQQAAVTR WGPRTSCPVHSPFRVRVPNG SICSGH in isoform A and isoform G. 1 PublicationAdd BLAST126
Alternative sequenceiVSP_008090584E → ELFRIRPAPVL in isoform A and isoform G. 1 Publication1
Alternative sequenceiVSP_041854663Q → QGEPGAGCSAHADGCDGSA in isoform G. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53190 mRNA Translation: AAC80169.1
X94264 mRNA Translation: CAA63940.1 Frameshift.
AE014297 Genomic DNA Translation: AAF55920.3
AE014297 Genomic DNA Translation: AAF55921.2
AE014297 Genomic DNA Translation: AAF55922.2
AY094916 mRNA Translation: AAM11269.1 Sequence problems.
BT003532 mRNA Translation: AAO39536.1 Frameshift.
BT003550 mRNA Translation: AAO39554.1
BT046172 mRNA Translation: ACI49771.1
PIRiS69210
RefSeqiNP_001097862.1, NM_001104392.3 [Q24210-2]
NP_524441.2, NM_079717.4 [Q24210-3]
NP_732661.1, NM_169970.2 [Q24210-2]
NP_732662.2, NM_169971.3 [Q24210-4]
UniGeneiDm.7404

Genome annotation databases

EnsemblMetazoaiFBtr0084161; FBpp0083559; FBgn0013759 [Q24210-3]
FBtr0084162; FBpp0083560; FBgn0013759 [Q24210-2]
FBtr0112872; FBpp0111785; FBgn0013759 [Q24210-2]
FBtr0301328; FBpp0290543; FBgn0013759 [Q24210-4]
GeneIDi42567
KEGGidme:Dmel_CG6703

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53190 mRNA Translation: AAC80169.1
X94264 mRNA Translation: CAA63940.1 Frameshift.
AE014297 Genomic DNA Translation: AAF55920.3
AE014297 Genomic DNA Translation: AAF55921.2
AE014297 Genomic DNA Translation: AAF55922.2
AY094916 mRNA Translation: AAM11269.1 Sequence problems.
BT003532 mRNA Translation: AAO39536.1 Frameshift.
BT003550 mRNA Translation: AAO39554.1
BT046172 mRNA Translation: ACI49771.1
PIRiS69210
RefSeqiNP_001097862.1, NM_001104392.3 [Q24210-2]
NP_524441.2, NM_079717.4 [Q24210-3]
NP_732661.1, NM_169970.2 [Q24210-2]
NP_732662.2, NM_169971.3 [Q24210-4]
UniGeneiDm.7404

3D structure databases

ProteinModelPortaliQ24210
SMRiQ24210
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67530, 14 interactors
DIPiDIP-19769N
IntActiQ24210, 7 interactors
STRINGi7227.FBpp0303394

Protein family/group databases

TCDBi9.B.106.3.2 the pock size-determining protein (psdp) family

Proteomic databases

PaxDbiQ24210
PRIDEiQ24210

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084161; FBpp0083559; FBgn0013759 [Q24210-3]
FBtr0084162; FBpp0083560; FBgn0013759 [Q24210-2]
FBtr0112872; FBpp0111785; FBgn0013759 [Q24210-2]
FBtr0301328; FBpp0290543; FBgn0013759 [Q24210-4]
GeneIDi42567
KEGGidme:Dmel_CG6703

Organism-specific databases

CTDi8573
FlyBaseiFBgn0013759 CASK

Phylogenomic databases

eggNOGiKOG0033 Eukaryota
KOG0609 Eukaryota
COG0194 LUCA
GeneTreeiENSGT00760000118866
InParanoidiQ24210
KOiK06103
OrthoDBiEOG091G065I
PhylomeDBiQ24210

Enzyme and pathway databases

BRENDAi2.7.11.17 1994
ReactomeiR-DME-212676 Dopamine Neurotransmitter Release Cycle
R-DME-6794361 Neurexins and neuroligins
SignaLinkiQ24210

Miscellaneous databases

ChiTaRSiCASK fly
GenomeRNAii42567
PROiPR:Q24210

Gene expression databases

BgeeiFBgn0013759 Expressed in 28 organ(s), highest expression level in head
ExpressionAtlasiQ24210 baseline and differential
GenevisibleiQ24210 DM

Family and domain databases

CDDicd12081 SH3_CASK, 1 hit
InterProiView protein in InterPro
IPR035473 CASK_SH3
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR020590 Guanylate_kinase_CS
IPR011009 Kinase-like_dom_sf
IPR014775 L27_C
IPR004172 L27_dom
IPR036892 L27_dom_sf
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000719 Prot_kinase_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF02828 L27, 2 hits
PF00595 PDZ, 1 hit
PF00069 Pkinase, 1 hit
PF07653 SH3_2, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00569 L27, 2 hits
SM00228 PDZ, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF101288 SSF101288, 2 hits
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 1 hit
SSF52540 SSF52540, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
PS51022 L27, 2 hits
PS50106 PDZ, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCSKP_DROME
AccessioniPrimary (citable) accession number: Q24210
Secondary accession number(s): B5X553
, Q24272, Q86P03, Q86P17, Q8SX09, Q9VD77, Q9VD78, Q9VD79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 22, 2003
Last modified: September 12, 2018
This is version 191 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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