UniProtKB - Q24206 (BRC4_DROME)
Protein
Broad-complex core protein isoform 6
Gene
br
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Broad-complex proteins are required for puffing and transcription of salivary gland late genes during metamorphosis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 710 – 733 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 740 – 763 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription repressor activity Source: FlyBase
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- autophagy Source: FlyBase
- cellular response to ecdysone Source: FlyBase
- compound eye photoreceptor fate commitment Source: FlyBase
- dendrite morphogenesis Source: FlyBase
- dorsal appendage formation Source: FlyBase
- eclosion Source: FlyBase
- gravitaxis Source: FlyBase
- male genitalia morphogenesis Source: FlyBase
- muscle fiber development Source: FlyBase
- negative regulation of gene expression Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- oogenesis Source: FlyBase
- ovarian follicle cell development Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- progression of morphogenetic furrow involved in compound eye morphogenesis Source: FlyBase
- regulation of development, heterochronic Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to ecdysone Source: FlyBase
- response to symbiont Source: FlyBase
- salivary gland histolysis Source: FlyBase
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
SignaLinki | Q24206 |
Names & Taxonomyi
Protein namesi | Recommended name: Broad-complex core protein isoform 6 |
Gene namesi | Name:br Synonyms:Br-C ORF Names:CG11491 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0283451, br |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: FlyBase
Other locations
- neuronal cell body Source: FlyBase
- polytene chromosome Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047070 | 1 – 880 | Broad-complex core protein isoform 6Add BLAST | 880 |
Proteomic databases
PaxDbi | Q24206 |
PRIDEi | Q24206 |
Expressioni
Developmental stagei
Accumulates to a high level at the beginning of the ecdysone response, during the metamorphosis of imaginal disks in puff stage 1, and abruptly disappears after several hours.1 Publication
Inductioni
Induced as a primary response to 20-hydroxyecdysone in third instar larval imaginal disks.
Gene expression databases
Bgeei | FBgn0000210, Expressed in wing disc (Drosophila) and 27 other tissues |
ExpressionAtlasi | Q24206, baseline and differential |
Genevisiblei | Q24206, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 69091, 41 interactors |
DIPi | DIP-18557N |
IntActi | Q24206, 1 interactor |
STRINGi | 7227.FBpp0289656 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 97 | BTBPROSITE-ProRule annotationAdd BLAST | 66 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 203 – 207 | Poly-Ala | 5 | |
Compositional biasi | 265 – 268 | Poly-Asn | 4 | |
Compositional biasi | 458 – 466 | Poly-Asn | 9 | |
Compositional biasi | 584 – 589 | Poly-Pro | 6 | |
Compositional biasi | 618 – 621 | Poly-Ala | 4 | |
Compositional biasi | 798 – 803 | Poly-Ala | 6 | |
Compositional biasi | 821 – 833 | Poly-AlaAdd BLAST | 13 | |
Compositional biasi | 862 – 867 | Poly-Gln | 6 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 710 – 733 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 740 – 763 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QUP1, Eukaryota |
HOGENOMi | CLU_011721_0_0_1 |
InParanoidi | Q24206 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 2 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 6 (identifier: Q24206-1) [UniParc]FASTAAdd to basket
Also known as: BRCORE-Z4
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDDTQHFCLR WNNYQSSITS AFENLRDDEA FVDVTLACEG RSIKAHRVVL
60 70 80 90 100
SACSPYFREL LKSTPCKHPV ILLQDVNFMD LHALVEFIYH GEVNVHQKSL
110 120 130 140 150
QSFLKTAEVL RVSGLTQQQA EDTHSHLAQI QNLANSGGRT PLNTHTQSLP
160 170 180 190 200
HPHHGSLHDD GGSSTLFSRQ GAGSPPPTAV PSLPSHINNQ LLKRMAMMHR
210 220 230 240 250
SSAAAAAEET SHAFKRLRGS DNSLPLSGAV GSGSNNNSPD LPPLHARSAS
260 270 280 290 300
PQQTPADFST IKHHNNNNTP PLKEEKRNGP TGNGNSGNGN GNGNGASNGN
310 320 330 340 350
GISISDKLGS LTPSPLARAG ADDVKSEPMD MVCSNNNANA NDEHSNDSTG
360 370 380 390 400
EHDANRSSSG DGGKGSLSSG NDEEIGDGLA SHHAAPQFIM SPAENKMFHA
410 420 430 440 450
AAFNFPNIDP SALLGLNTQL QQSGDLAVSP QGGSTGSLLS GVIVPGGSGG
460 470 480 490 500
TPSNSSSNNN NNNSNNQQQK VEQQSSPHQL LQQQHHSTPH TNSPQLKQEQ
510 520 530 540 550
PKSGGGSCKS SDLHIAAGSE RSLSRSSQGM PDAGGHSATP SPTAAYHKRE
560 570 580 590 600
RERERERERE RERERERSLD HERDLERPGG TGSPPPPPPS HHSHFGQHPL
610 620 630 640 650
SLLPSHHQLH ATHHELSAAA AHHAHAHAHA AHAHALARAG SPMEHHHLLH
660 670 680 690 700
HRRASLSPSG AVSSASGAGG RGGGAGGPGG PGGSLLSSVR AQDVAQANRL
710 720 730 740 750
LLPLPLNACH RCDVCGKLLS TKLTLKRHKE QQHLQPLNNA VCNLCHKVFR
760 770 780 790 800
TLNSLNNHKS IYHRRQKNHH SYFHHGAGVS QAGSPGSRLH QSLSSLSAAA
810 820 830 840 850
AAANNSVNVG GGSVGGAGGN AVAAAAAAAA AAAELLLSPI VGAAAVAGGT
860 870 880
ASSTLQLAAA HQQQQQQSSP GIVKPCMDFL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD8FT05 | D8FT05_DROME | Broad, isoform O | br 2B5, 2Bc, A18, BR, Br | 663 | Annotation score: | ||
E1JJB2 | E1JJB2_DROME | Broad, isoform P | br 2B5, 2Bc, A18, BR, Br | 1,011 | Annotation score: | ||
Q9W572 | Q9W572_DROME | Broad, isoform C | br 2B5, 2Bc, A18, BR, Br | 880 | Annotation score: | ||
Q8IRX4 | Q8IRX4_DROME | Broad, isoform J | br 2B5, 2Bc, A18, BR, Br | 514 | Annotation score: | ||
M9MSM6 | M9MSM6_DROME | Broad, isoform M | br 2B5, 2Bc, A18, BR, Br | 724 | Annotation score: | ||
A0A6H2EEH5 | A0A6H2EEH5_DROME | Broad, isoform R | br 2B5, 2Bc, A18, BR, Br | 644 | Annotation score: |
Sequence cautioni
The sequence AAB09760 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 436 | G → D in AAB09760 (PubMed:8660872).Curated | 1 | |
Sequence conflicti | 621 | Missing (PubMed:8660872).Curated | 1 | |
Sequence conflicti | 624 | A → R in AAB09760 (PubMed:8660872).Curated | 1 | |
Sequence conflicti | 661 – 662 | AV → L (PubMed:8660872).Curated | 2 | |
Sequence conflicti | 678 | Missing (PubMed:8660872).Curated | 1 | |
Sequence conflicti | 722 – 723 | KL → NV in AAB09760 (PubMed:8660872).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51585 mRNA Translation: AAB09760.1 Frameshift. AE014298 Genomic DNA Translation: AAF45647.1 AL009146 Genomic DNA Translation: CAA15627.1 AY069756 mRNA Translation: AAL39901.1 |
RefSeqi | NP_001138144.1, NM_001144672.3 [Q24206-1] NP_001259134.1, NM_001272205.1 [Q24206-1] NP_726751.2, NM_166895.2 [Q24206-1] NP_726752.1, NM_166896.3 [Q24206-1] NP_726753.1, NM_166897.2 [Q24206-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0070261; FBpp0070251; FBgn0283451 [Q24206-1] FBtr0070263; FBpp0070253; FBgn0283451 [Q24206-1] FBtr0300427; FBpp0289656; FBgn0283451 [Q24206-1] FBtr0300428; FBpp0289657; FBgn0283451 [Q24206-1] FBtr0332293; FBpp0304572; FBgn0283451 [Q24206-1] |
GeneIDi | 44505 |
KEGGi | dme:Dmel_CG11491 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51585 mRNA Translation: AAB09760.1 Frameshift. AE014298 Genomic DNA Translation: AAF45647.1 AL009146 Genomic DNA Translation: CAA15627.1 AY069756 mRNA Translation: AAL39901.1 |
RefSeqi | NP_001138144.1, NM_001144672.3 [Q24206-1] NP_001259134.1, NM_001272205.1 [Q24206-1] NP_726751.2, NM_166895.2 [Q24206-1] NP_726752.1, NM_166896.3 [Q24206-1] NP_726753.1, NM_166897.2 [Q24206-1] |
3D structure databases
SMRi | Q24206 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 69091, 41 interactors |
DIPi | DIP-18557N |
IntActi | Q24206, 1 interactor |
STRINGi | 7227.FBpp0289656 |
Proteomic databases
PaxDbi | Q24206 |
PRIDEi | Q24206 |
Genome annotation databases
EnsemblMetazoai | FBtr0070261; FBpp0070251; FBgn0283451 [Q24206-1] FBtr0070263; FBpp0070253; FBgn0283451 [Q24206-1] FBtr0300427; FBpp0289656; FBgn0283451 [Q24206-1] FBtr0300428; FBpp0289657; FBgn0283451 [Q24206-1] FBtr0332293; FBpp0304572; FBgn0283451 [Q24206-1] |
GeneIDi | 44505 |
KEGGi | dme:Dmel_CG11491 |
Organism-specific databases
CTDi | 103946 |
FlyBasei | FBgn0283451, br |
Phylogenomic databases
eggNOGi | ENOG502QUP1, Eukaryota |
HOGENOMi | CLU_011721_0_0_1 |
InParanoidi | Q24206 |
Enzyme and pathway databases
SignaLinki | Q24206 |
Miscellaneous databases
BioGRID-ORCSi | 44505, 1 hit in 1 CRISPR screen |
ChiTaRSi | br, fly |
GenomeRNAii | 44505 |
Gene expression databases
Bgeei | FBgn0000210, Expressed in wing disc (Drosophila) and 27 other tissues |
ExpressionAtlasi | Q24206, baseline and differential |
Genevisiblei | Q24206, DM |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 2 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BRC4_DROME | |
Accessioni | Q24206Primary (citable) accession number: Q24206 Secondary accession number(s): O46064, Q9W575 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | February 10, 2021 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase