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Protein

Proteasome subunit alpha type-7-1A

Gene

Prosalpha4T1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-1A (EC:3.4.25.1)
Alternative name(s):
Testis-specific alpha4-t1 proteasome subunit
Testis-specific proteasome 28 kDa subunit 1A
Gene namesi
Name:Prosalpha4T1
Synonyms:Pros28.1A
ORF Names:CG17268
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0265606 Prosalpha4T1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241541 – 249Proteasome subunit alpha type-7-1AAdd BLAST249

Proteomic databases

PaxDbiQ24178
PRIDEiQ24178

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiFBgn0265606
ExpressionAtlasiQ24178 differential
GenevisibleiQ24178 DM

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

BioGridi67432, 1 interactor
DIPiDIP-19969N
IntActiQ24178, 3 interactors
STRINGi7227.FBpp0083336

Structurei

3D structure databases

ProteinModelPortaliQ24178
SMRiQ24178
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0183 Eukaryota
ENOG410XP21 LUCA
InParanoidiQ24178
KOiK02731
OMAiNTVREFF
OrthoDBiEOG091G0GX6
PhylomeDBiQ24178

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR037560 Proteasome_alpha_7-like
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PANTHERiPTHR11599:SF15 PTHR11599:SF15, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q24178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRYGRALT IFSPDGHLLQ VEYAQEAVRK GSTAVGVRGA NCVVLGVEKS
60 70 80 90 100
SVSEMQEDRT VRKISMLDRH VALAFAGLTA DARILINRGQ VECQSHRLNF
110 120 130 140 150
ENQVTLEYIT RYLAQLKQKY TQCNGRRPFG ISCLIGGIDA DGSARLFHTE
160 170 180 190 200
PSGIFHEYKA TATGRWANTV REFFEKAYSD HEVTTKCDAI KLAMRALLEV
210 220 230 240
TQMSQMRLEV AVLENGKPMK MLDSVVISEI VKIVQNEKEL QAKAHKMKR
Length:249
Mass (Da):27,940
Last modified:December 8, 2000 - v2
Checksum:i18B642DE3F1AE453
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24A → G in AAC47280 (PubMed:8878681).Curated1
Sequence conflicti123C → V in AAC47280 (PubMed:8878681).Curated1
Sequence conflicti127 – 128RP → PS in AAC47280 (PubMed:8878681).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29R → P in strain: CPA-46. 1
Natural varianti236N → K in strain: CPA-46. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46008 Genomic DNA Translation: AAC47280.1
AY542403 Genomic DNA Translation: AAS86247.1
AY542404 Genomic DNA Translation: AAS86248.1
AY542405 Genomic DNA Translation: AAS86249.1
AY542406 Genomic DNA Translation: AAS86250.1
AY542407 Genomic DNA Translation: AAS86251.1
AY542408 Genomic DNA Translation: AAS86252.1
AY542409 Genomic DNA Translation: AAS86253.1
AE014297 Genomic DNA Translation: AAF55802.1
AY075276 mRNA Translation: AAL68143.1
PIRiS72225
RefSeqiNP_650910.1, NM_142653.3
UniGeneiDm.5563

Genome annotation databases

EnsemblMetazoaiFBtr0083928; FBpp0083336; FBgn0265606
GeneIDi42457
KEGGidme:Dmel_CG17268

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPSA72_DROME
AccessioniPrimary (citable) accession number: Q24178
Secondary accession number(s): Q6QH19, Q8T8U0, Q9VDJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 8, 2000
Last modified: July 18, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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