Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase Shark

Gene

Shark

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Following axon injury, required for recruitment of drpr and glial cells to severed axons and for glial clearance of severed axons from the central nervous system (PubMed:18432193). Together with Src42a and drpr, promotes the migration of macrophages to sites of wounding as part of a signaling cascade where Scr42a detects production of hydrogen peroxide at wound sites which triggers phosphorylation of drpr and subsequent recruitment and activation of shark (PubMed:26028435). May be involved in signal transduction on the apical surface of ectodermal epithelial cells, regulating their polarity during invagination (PubMed:7892198). Crumbs (crb) may be the intracellular signal (PubMed:7892198).3 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei698ATPPROSITE-ProRule annotation1
Active sitei789Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi668 – 676ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • apoptotic cell clearance Source: FlyBase
  • cell migration Source: GO_Central
  • dorsal appendage formation Source: FlyBase
  • dorsal closure Source: FlyBase
  • dorsal closure, elongation of leading edge cells Source: FlyBase
  • eggshell chorion assembly Source: FlyBase
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • maintenance of epithelial cell apical/basal polarity Source: UniProtKB
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • protein phosphorylation Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1994
ReactomeiR-DME-354192 Integrin alphaIIb beta3 signaling
R-DME-912631 Regulation of signaling by CBL
SignaLinkiQ24145

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Shark1 Publication (EC:2.7.10.2)
Gene namesi
Name:Shark1 PublicationImported
Synonyms:Tk71 Publication
ORF Names:CG18247Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015295 Shark

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Strong reduction in the number of macrophages recruited to wound sites (PubMed:26028435). RNAi-mediated knockdown in glial cells abolishes a number of events which normally occur following axon injury including drpr localization at severed axons, glial recruitment to severed axons, up-regulation of drpr in glial cells and clearance of axonal debris from the central nervous system (PubMed:18432193).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi698K → R: Kinase-dead. No effect on interaction with drpr. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881371 – 939Tyrosine-protein kinase SharkAdd BLAST939

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei927PhosphotyrosineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ24145
PRIDEiQ24145

Expressioni

Tissue specificityi

Gastrulation embryos show expression in ectodermal cells along the cephalic furrow and ventral midline. Proctodeum, stomodeum and their derived structures (foregut, atrium, pharynx, esophagus and hindgut) continue to show expression from stage 8-9 to late embryos. Other ectodermally derived structures (frontal sac, salivary gland and labium) and developing tracheal system also show expression.1 Publication

Developmental stagei

Embryos only.1 Publication

Gene expression databases

BgeeiFBgn0015295
ExpressionAtlasiQ24145 baseline and differential
GenevisibleiQ24145 DM

Interactioni

Subunit structurei

Interacts with drpr; this is required for the recruitment of drpr and glial cells to severed axons and for the phagocytosis of axonal debris by glial cells following axon injury.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68999, 3 interactors
DIPiDIP-59843N
IntActiQ24145, 2 interactors
STRINGi7227.FBpp0086382

Structurei

3D structure databases

ProteinModelPortaliQ24145
SMRiQ24145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 106SH2 1PROSITE-ProRule annotationCuratedAdd BLAST97
Repeati153 – 185ANK 1CuratedAdd BLAST33
Repeati186 – 218ANK 2CuratedAdd BLAST33
Repeati220 – 252ANK 3CuratedAdd BLAST33
Domaini288 – 403SH2 2PROSITE-ProRule annotationCuratedAdd BLAST116
Domaini662 – 921Protein kinasePROSITE-ProRule annotationAdd BLAST260

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, SH2 domain

Phylogenomic databases

eggNOGiENOG410IH0T Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000118799
InParanoidiQ24145
KOiK17512
OMAiLCPAYIY
OrthoDBiEOG091G04J0
PhylomeDBiQ24145

Family and domain databases

CDDicd00204 ANK, 1 hit
cd10347 SH2_Nterm_shark_like, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR035061 Shark-like_SH2_N
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 2 hits
PRINTSiPR00401 SH2DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SM00252 SH2, 2 hits
SM00219 TyrKc, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF55550 SSF55550, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 2 hits

Sequencei

Sequence statusi: Complete.

Q24145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRDSDPMKW YHGNLSREAA DELLKQGYED GTFLVRESST AAGDFVLSLL
60 70 80 90 100
CQGEVCHYQV RRHGGEDAFF SIDDKVQTKI LHGLDTLVDY YQQAANGLPT
110 120 130 140 150
KLTVPLIRDL PPHNTRSHGV TNLLHRATSK NESKVVFELL KCGYRNFDAK
160 170 180 190 200
NQDGQTALHL AALHSDEDIL KHLLNAKVQV NSSDSFGCQP LHYAARSKPA
210 220 230 240 250
SFIRTLISAQ ANVQGRNIDN GYVPLHEAAK HGNLEAVQEL LLAEAPPLPR
260 270 280 290 300
TSSGEFPFDL AKEAGQTAVE EFLLNYKLPP ANTTRDQWYH GTLTREEAVA
310 320 330 340 350
ILKKHAKELL AKQPEVDTSG CFLVRYSESP AASGLVLTLL CDQVVKNFRI
360 370 380 390 400
SQADLYQNGN KVQSGGSKFL YIDDGPYWPS VEHLIAHFMR FSYGLPVSLK
410 420 430 440 450
YPVPPQPKPE VPSFATIPRS NMKPKAASPA TPPTPVSPHS HHQHPHVPAL
460 470 480 490 500
TITKKKQKEN SSSMFNTLRL TSPKKALFDM NSLRKNKSKG KRSDSESSVS
510 520 530 540 550
GSLAGTEQEL QAAAPMLKSL SFSTEFSTFN ADGVTGSGAA AAGEVYNVPR
560 570 580 590 600
NNTPIEIDLP PIAQKTEAEV EYFTKSDVAI ERERAGQWIG NGYQPTMDVL
610 620 630 640 650
SLLDQQIKAP AVARLNSLGP NASTESEMAS YLHRKCSGTP STPSATEVEA
660 670 680 690 700
AKLRFFIEPE KLVLDREIGH GEFGSVHSGW LLRKSGAGEE SRLEVAIKML
710 720 730 740 750
SDEHSNKQEF LREASVMMRL EHKCIVRLIG IAKGEMLMMV QELAPLGSML
760 770 780 790 800
QYILDHGHEI TANAELKVWA SQIACGMHYL ESQHFVHRDL AARNILLTAR
810 820 830 840 850
HQAKISDFGM SRSLRPGSTE YQFTQGGRWP IRWYAPESFN LGIFSHASDV
860 870 880 890 900
WSFGVTIWEM FSLGAPPYGE ISNVDAIKLV DSGERLPQPN LCPAYIYAVM
910 920 930
QSCWKERPKD RPTFVYLTEF FARDPDYQNL PELVQTVHI
Length:939
Mass (Da):104,273
Last modified:October 1, 2000 - v2
Checksum:i37CC2C3DA25D3F52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19A → V in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti39S → R in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti50L → F in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti85D → E in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti129S → T in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti199P → T in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti341C → S in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti505 – 506GT → RA in AAA79851 (PubMed:7892198).Curated2
Sequence conflicti597M → V in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti611A → P in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti732A → S in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti898A → P in AAA79851 (PubMed:7892198).Curated1
Sequence conflicti935 – 939QTVHI → KRFTFNPVSIFHFFRC in AAA79851 (PubMed:7892198).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37773 mRNA Translation: AAA79851.1
AE013599 Genomic DNA Translation: AAF58044.1
AY051937 mRNA Translation: AAK93361.1
S55982 Genomic DNA Translation: AAB19909.1
PIRiS18015
RefSeqiNP_524743.2, NM_080004.4
UniGeneiDm.6764

Genome annotation databases

EnsemblMetazoaiFBtr0087244; FBpp0086382; FBgn0015295
GeneIDi44353
KEGGidme:Dmel_CG18247

Similar proteinsi

Entry informationi

Entry nameiSHARK_DROME
AccessioniPrimary (citable) accession number: Q24145
Secondary accession number(s): Q26299, Q9V7K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health