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UniProtKB - Q24141 (SGO1_DROME)
Protein
Shugoshin
Gene
mei-S332
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Plays a central role in chromosome cohesion during meiosis and mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by protecting or Rad21 from cleavage by Sse/separase. Required during meiosis in both males and females.
4 PublicationsGO - Biological processi
- cell division Source: UniProtKB-KW
- female meiotic nuclear division Source: FlyBase
- male meiosis sister chromatid cohesion Source: FlyBase
- male meiotic nuclear division Source: FlyBase
- meiotic chromosome segregation Source: FlyBase
- sister chromatid cohesion Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis |
Enzyme and pathway databases
SignaLinki | Q24141 |
Names & Taxonomyi
Protein namesi | Recommended name: ShugoshinAlternative name(s): Meiotic protein S332 |
Gene namesi | Name:mei-S332 ORF Names:CG5303 |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002715, mei-S332 |
VEuPathDBi | VectorBase:FBgn0002715 |
Subcellular locationi
Other locations
- centromere 4 Publications
Note: Localizes to the centromere of meiotic and mitotic chromosomes (PubMed:9490715, PubMed:10654079). During meiosis, it assembles onto centromeres during prometaphase I, remains attached until anaphase II, during which it is displaced from centromeres without being degraded (PubMed:9490715). Localization onto centromeres is independent of kinetochore formation and cohesin complex (PubMed:10985388).3 Publications
Nucleus
- nucleus Source: InterPro
Other locations
- chromosome Source: FlyBase
- chromosome, centromeric region Source: UniProtKB
- condensed chromosome, centromeric region Source: FlyBase
- pericentric heterochromatin Source: FlyBase
Keywords - Cellular componenti
Centromere, ChromosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | N → I in mei-S332-9; induces missegregation of chromatids during meiosis. 1 Publication | 1 | |
Mutagenesisi | 28 | G → D in mei-S332-3; induces missegregation of chromatids during meiosis. 2 Publications | 1 | |
Mutagenesisi | 34 | V → E in mei-S332-8; induces missegregation of chromatids during meiosis. 2 Publications | 1 | |
Mutagenesisi | 112 | L → M in mei-S332-4; induces missegregation of chromatids during meiosis; when associated with H-. 1 Publication | 1 | |
Mutagenesisi | 124 – 126 | SSS → AAA: Does not stably associate with centromeres during mitosis. 1 Publication | 3 | |
Mutagenesisi | 234 | S → A: Does not affect phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 277 | S → F in mei-S332-5; induces missegregation of chromatids during meiosis. 1 Publication | 1 | |
Mutagenesisi | 331 | T → A: Affects phosphorylation and localization to centromeres. 1 Publication | 1 | |
Mutagenesisi | 375 | C → Y in mei-S332-2; induces missegregation of chromatids during meiosis. 1 Publication | 1 | |
Mutagenesisi | 377 | P → H in mei-S332-4; induces missegregation of chromatids during meiosis; when associated with M-112. 1 Publication | 1 | |
Mutagenesisi | 382 | E → K in mei-S332-10; induces missegregation of chromatids during meiosis. 1 Publication | 1 | |
Mutagenesisi | 384 | S → R in mei-S332-6; induces missegregation of chromatids during meiosis. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055443 | 1 – 401 | ShugoshinAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 124 | Phosphoserine; by AurBCurated | 1 | |
Modified residuei | 125 | Phosphoserine; by AurBCurated | 1 | |
Modified residuei | 126 | Phosphoserine; by AurBCurated | 1 | |
Modified residuei | 331 | Phosphothreonine; by PLK11 Publication | 1 |
Post-translational modificationi
Phosphorylation by polo-like kinase (PLK) on Thr-331 antagonizes cohesive function. Phosphorylation on Thr-331 at the metaphase anaphase transition leads to its dissociation from centromeres. In contrast, phosphorylation by aurB/ial on either Ser-124, Ser-125 or Ser-126 is required for association with centromeres.2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q24141 |
PTM databases
iPTMneti | Q24141 |
Expressioni
Developmental stagei
Expressed both maternally and zygotically. Present throughout embryogenesis and larval stages.
Gene expression databases
Bgeei | FBgn0002715, Expressed in embryo and 36 other tissues |
ExpressionAtlasi | Q24141, baseline and differential |
Genevisiblei | Q24141, DM |
Interactioni
Subunit structurei
Homodimer.
Interacts with Incenp.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 63143, 43 interactors |
DIPi | DIP-17412N |
IntActi | Q24141, 10 interactors |
STRINGi | 7227.FBpp0071675 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 197 | DisorderedSequence analysisAdd BLAST | 116 | |
Regioni | 338 – 401 | DisorderedSequence analysisAdd BLAST | 64 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 3 – 49 | Sequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 97 – 111 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 118 – 146 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 148 – 172 | Basic and acidic residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 173 – 187 | Acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 354 – 386 | Polar residuesSequence analysisAdd BLAST | 33 |
Sequence similaritiesi
Belongs to the shugoshin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502TCIV, Eukaryota |
HOGENOMi | CLU_632032_0_0_1 |
InParanoidi | Q24141 |
OMAi | SENTDMS |
OrthoDBi | 1145243at2759 |
PhylomeDBi | Q24141 |
Family and domain databases
InterProi | View protein in InterPro IPR011515, Shugoshin_C IPR011516, Shugoshin_N |
Pfami | View protein in Pfam PF07557, Shugoshin_C, 1 hit PF07558, Shugoshin_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q24141-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSKVEQQYK LLNAELMDQV QKQRLEIGEY RKRVISLERE IMDIREEHVL
60 70 80 90 100
QNHRQRMENI SIVRSLMLSL NVDSDSLAVR QEPAPAAQIN RPSGPRRSSR
110 120 130 140 150
EICKDMRRTC ALARTTRPIS PRRSSSVTST VSSTSRRSSA EVQSEVVTTR
160 170 180 190 200
IPEDRRANKP TPPPRRPAEL VFDEDDSDDD FDEAVSPVEE TQTEQNEENN
210 220 230 240 250
RLFSIIEENG SEGESTDSSS SCEAIYCDTT FESSPPNAQV TVTPSGRALR
260 270 280 290 300
EVDTNIPVAV SLSRGKETGK GSWLAISVAV EDSPQEPSIQ CPRLAVTRPS
310 320 330 340 350
QSSGIFPDVN GLTPRRSLFN GIGKMAGSTS TPKSFLVEEM PSIRTRSRTA
360 370 380 390 400
ANKKSENTDM SSSFCNNSAR PSRSCRPTSL VEPSLKNKLR NGSKGKAKAK
K
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4LG47 | A0A0B4LG47_DROME | Meiotic from via salaria 332, isofo... | mei-S332 BEST:LD13774, Dmel\CG5303, MEI-S322, mei-S322, MEI-S332 | 401 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36583 mRNA Translation: AAA87038.1 AE013599 Genomic DNA Translation: AAF46802.1 AY058633 mRNA Translation: AAL13862.1 |
PIRi | A57226 |
RefSeqi | NP_001286705.1, NM_001299776.1 NP_476634.1, NM_057286.5 |
Genome annotation databases
EnsemblMetazoai | FBtr0071761; FBpp0071675; FBgn0002715 FBtr0342979; FBpp0309743; FBgn0002715 |
GeneIDi | 37523 |
KEGGi | dme:Dmel_CG5303 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36583 mRNA Translation: AAA87038.1 AE013599 Genomic DNA Translation: AAF46802.1 AY058633 mRNA Translation: AAL13862.1 |
PIRi | A57226 |
RefSeqi | NP_001286705.1, NM_001299776.1 NP_476634.1, NM_057286.5 |
3D structure databases
AlphaFoldDBi | Q24141 |
SMRi | Q24141 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63143, 43 interactors |
DIPi | DIP-17412N |
IntActi | Q24141, 10 interactors |
STRINGi | 7227.FBpp0071675 |
PTM databases
iPTMneti | Q24141 |
Proteomic databases
PaxDbi | Q24141 |
Protocols and materials databases
DNASUi | 37523 |
Genome annotation databases
EnsemblMetazoai | FBtr0071761; FBpp0071675; FBgn0002715 FBtr0342979; FBpp0309743; FBgn0002715 |
GeneIDi | 37523 |
KEGGi | dme:Dmel_CG5303 |
Organism-specific databases
CTDi | 37523 |
FlyBasei | FBgn0002715, mei-S332 |
VEuPathDBi | VectorBase:FBgn0002715 |
Phylogenomic databases
eggNOGi | ENOG502TCIV, Eukaryota |
HOGENOMi | CLU_632032_0_0_1 |
InParanoidi | Q24141 |
OMAi | SENTDMS |
OrthoDBi | 1145243at2759 |
PhylomeDBi | Q24141 |
Enzyme and pathway databases
SignaLinki | Q24141 |
Miscellaneous databases
BioGRID-ORCSi | 37523, 0 hits in 1 CRISPR screen |
ChiTaRSi | mei-S332, fly |
GenomeRNAii | 37523 |
PROi | PR:Q24141 |
Gene expression databases
Bgeei | FBgn0002715, Expressed in embryo and 36 other tissues |
ExpressionAtlasi | Q24141, baseline and differential |
Genevisiblei | Q24141, DM |
Family and domain databases
InterProi | View protein in InterPro IPR011515, Shugoshin_C IPR011516, Shugoshin_N |
Pfami | View protein in Pfam PF07557, Shugoshin_C, 1 hit PF07558, Shugoshin_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SGO1_DROME | |
Accessioni | Q24141Primary (citable) accession number: Q24141 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families