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UniProtKB - Q24120 (CAPU_DROME)
Protein
Protein cappuccino
Gene
capu
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Acts as an actin nucleation factor and promotes assembly of actin filaments together with spir. May play a role in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport.
1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- microtubule binding Source: FlyBase
GO - Biological processi
- actin filament-based process Source: FlyBase
- actin filament organization Source: FlyBase
- actin nucleation Source: FlyBase
- chorion-containing eggshell formation Source: FlyBase
- oogenesis Source: FlyBase
- pole plasm assembly Source: FlyBase
- pole plasm oskar mRNA localization Source: FlyBase
- pole plasm RNA localization Source: FlyBase
- protein transport Source: UniProtKB-KW
Keywordsi
Molecular function | Actin-binding, Developmental protein |
Biological process | Protein transport, Stress response, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Protein cappuccino |
Gene namesi | Name:capu ORF Names:CG3399 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000256, capu |
VEuPathDBi | VectorBase:FBgn0000256 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Other locations
- Membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
- Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Recruited to membranes via its interaction with spir.
Cytoskeleton
- actin filament Source: InterPro
Cytosol
- cytosol Source: UniProtKB-SubCell
Other locations
- cell cortex Source: FlyBase
- cytoplasm Source: FlyBase
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1049 | K → A or D: Abolishes interaction with spir. 1 Publication | 1 | |
Mutagenesisi | 1051 | R → A or D: Abolishes interaction with spir. 1 Publication | 1 | |
Mutagenesisi | 1055 | R → A or D: Abolishes interaction with spir. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194884 | 1 – 1059 | Protein cappuccinoAdd BLAST | 1059 |
Proteomic databases
PaxDbi | Q24120 |
Expressioni
Gene expression databases
Bgeei | FBgn0000256, Expressed in testis and 48 other tissues |
ExpressionAtlasi | Q24120, baseline and differential |
Genevisiblei | Q24120, DM |
Interactioni
Subunit structurei
Interacts with wash.
Interacts with spir.
2 PublicationsGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- microtubule binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 59814, 11 interactors |
IntActi | Q24120, 3 interactors |
STRINGi | 7227.FBpp0288517 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 480 – 560 | FH1Add BLAST | 81 | |
Domaini | 585 – 1032 | FH2PROSITE-ProRule annotationAdd BLAST | 448 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 62 – 146 | DisorderedSequence analysisAdd BLAST | 85 | |
Regioni | 448 – 647 | DisorderedSequence analysisAdd BLAST | 200 | |
Regioni | 1049 – 1059 | Important for interaction with spirAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 62 – 126 | Polar residuesSequence analysisAdd BLAST | 65 | |
Compositional biasi | 131 – 145 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 454 – 468 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 482 – 559 | Pro residuesSequence analysisAdd BLAST | 78 | |
Compositional biasi | 616 – 632 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1922, Eukaryota |
HOGENOMi | CLU_006372_0_0_1 |
InParanoidi | Q24120 |
OrthoDBi | 263102at2759 |
Family and domain databases
Gene3Di | 1.20.58.2220, 1 hit |
InterProi | View protein in InterPro IPR015425, FH2_Formin IPR042201, FH2_Formin_sf IPR001265, Formin_Cappuccino_subfam |
Pfami | View protein in Pfam PF02181, FH2, 1 hit |
PRINTSi | PR00828, FORMIN |
SMARTi | View protein in SMART SM00498, FH2, 1 hit |
PROSITEi | View protein in PROSITE PS51444, FH2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q24120-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALQLGKKLA QVLGSGAGSP LTPGTMEPCA AGSGSPLANG ELFNVSKAKK
60 70 80 90 100
VELQNLSSRF TAAVTQTPPG VTSSTPNESG VTGPAGPLGA TTSSPSLETQ
110 120 130 140 150
STVIISFKSS QTPVQSQTNS AASENVEDDT APLPLPPPPP GFGTPTTPLL
160 170 180 190 200
SSNVLKKVAS FTVEKSSAGN NSSNPPNLCP TSDETTLLAT PCSSSLTVAT
210 220 230 240 250
LPPEIAVGAA AGGVAGGAGS RRGSSYVPEK LSFAAYEKFE GQMLIKWLIS
260 270 280 290 300
TMQSNPKSSS GDANQELFNT LALQFCNNLK YVGVLKQISN EHLDCGFSPY
310 320 330 340 350
EMYQWTHTEQ PTTSLPLTPG KLDKVAAWPF SSTPSGIRAL ESASLASLGA
360 370 380 390 400
GGVAGSLATI ATASTASSDN QKTLQQILKK RLLNCTTLAE VHAVVNELLS
410 420 430 440 450
SVDEPPRRPS KRCVNLTELL NASEATVYEY NKTGAEGCVK SFTDAETQTE
460 470 480 490 500
SEDCEGTCKC GQSSTKVSDN ESAKEDGEKP HAVAPPPPPP PPPLHAFVAP
510 520 530 540 550
PPPPPPPPPP PPPLANYGAP PPPPPPPPGS GSAPPPPPPA PIEGGGGIPP
560 570 580 590 600
PPPPMSASPS KTTISPAPLP DPAEGNWFHR TNTMRKSAVN PPKPMRPLYW
610 620 630 640 650
TRIVTSAPPA PRPPSVANST DSTENSGSSP DEPPAANGAD APPTAPPATK
660 670 680 690 700
EIWTEIEETP LDNIDEFTEL FSRQAIAPVS KPKELKVKRA KSIKVLDPER
710 720 730 740 750
SRNVGIIWRS LHVPSSEIEH AIYHIDTSVV SLEALQHMSN IQATEDELQR
760 770 780 790 800
IKEAAGGDIP LDHPEQFLLD ISLISMASER ISCIVFQAEF EESVTLLFRK
810 820 830 840 850
LETVSQLSQQ LIESEDLKLV FSIILTLGNY MNGGNRQRGQ ADGFNLDILG
860 870 880 890 900
KLKDVKSKES HTTLLHFIVR TYIAQRRKEG VHPLEIRLPI PEPADVERAA
910 920 930 940 950
QMDFEEVQQQ IFDLNKKFLG CKRTTAKVLA ASRPEIMEPF KSKMEEFVEG
960 970 980 990 1000
ADKSMAKLHQ SLDECRDLFL ETMRFYHFSP KACTLTLAQC TPDQFFEYWT
1010 1020 1030 1040 1050
NFTNDFKDIW KKEITSLLNE LMKKSKQAQI ESRRNVSTKV EKSGRISLKE
RMLMRRSKN
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7Z014 | B7Z014_DROME | Cappuccino, isoform G | capu capp, Capu, capu-RG, CG15420, Dmel\CG3399 | 932 | Annotation score: | ||
Q8IQ12 | Q8IQ12_DROME | Cappuccino, isoform J | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,089 | Annotation score: | ||
B7Z010 | B7Z010_DROME | Cappuccino, isoform E | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,280 | Annotation score: | ||
B7Z011 | B7Z011_DROME | Cappuccino, isoform F | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,361 | Annotation score: | ||
B7Z012 | B7Z012_DROME | Cappuccino, isoform H | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,107 | Annotation score: | ||
B7Z013 | B7Z013_DROME | Cappuccino, isoform I | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,298 | Annotation score: | ||
Q9VQV9 | Q9VQV9_DROME | Cappuccino, isoform D | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,207 | Annotation score: | ||
Q8MRP5 | Q8MRP5_DROME | Cappuccino, isoform B | capu capp, Capu, CG15420, Dmel\CG3399, CG3399 | 1,049 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 260 | S → C in AAC46925 (PubMed:7590229).Curated | 1 | |
Sequence conflicti | 364 | S → T in AAC46925 (PubMed:7590229).Curated | 1 | |
Sequence conflicti | 386 | T → S in AAC46925 (PubMed:7590229).Curated | 1 | |
Sequence conflicti | 471 | E → K in AAC46925 (PubMed:7590229).Curated | 1 | |
Sequence conflicti | 495 | H → P in AAC46925 (PubMed:7590229).Curated | 1 | |
Sequence conflicti | 513 | Missing in AAC46925 (PubMed:7590229).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34258 mRNA Translation: AAC46925.1 AE014134 Genomic DNA Translation: AAF51054.1 |
PIRi | T13286 |
RefSeqi | NP_476966.1, NM_057618.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0077513; FBpp0077202; FBgn0000256 |
GeneIDi | 33611 |
KEGGi | dme:Dmel_CG3399 |
UCSCi | CG3399-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34258 mRNA Translation: AAC46925.1 AE014134 Genomic DNA Translation: AAF51054.1 |
PIRi | T13286 |
RefSeqi | NP_476966.1, NM_057618.3 |
3D structure databases
AlphaFoldDBi | Q24120 |
SMRi | Q24120 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59814, 11 interactors |
IntActi | Q24120, 3 interactors |
STRINGi | 7227.FBpp0288517 |
Proteomic databases
PaxDbi | Q24120 |
Protocols and materials databases
DNASUi | 33611 |
Genome annotation databases
EnsemblMetazoai | FBtr0077513; FBpp0077202; FBgn0000256 |
GeneIDi | 33611 |
KEGGi | dme:Dmel_CG3399 |
UCSCi | CG3399-RA, d. melanogaster |
Organism-specific databases
CTDi | 33611 |
FlyBasei | FBgn0000256, capu |
VEuPathDBi | VectorBase:FBgn0000256 |
Phylogenomic databases
eggNOGi | KOG1922, Eukaryota |
HOGENOMi | CLU_006372_0_0_1 |
InParanoidi | Q24120 |
OrthoDBi | 263102at2759 |
Miscellaneous databases
BioGRID-ORCSi | 33611, 0 hits in 3 CRISPR screens |
ChiTaRSi | capu, fly |
GenomeRNAii | 33611 |
PROi | PR:Q24120 |
Gene expression databases
Bgeei | FBgn0000256, Expressed in testis and 48 other tissues |
ExpressionAtlasi | Q24120, baseline and differential |
Genevisiblei | Q24120, DM |
Family and domain databases
Gene3Di | 1.20.58.2220, 1 hit |
InterProi | View protein in InterPro IPR015425, FH2_Formin IPR042201, FH2_Formin_sf IPR001265, Formin_Cappuccino_subfam |
Pfami | View protein in Pfam PF02181, FH2, 1 hit |
PRINTSi | PR00828, FORMIN |
SMARTi | View protein in SMART SM00498, FH2, 1 hit |
PROSITEi | View protein in PROSITE PS51444, FH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPU_DROME | |
Accessioni | Q24120Primary (citable) accession number: Q24120 Secondary accession number(s): Q9VQV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families