UniProtKB - Q24087 (XPF_DROME)
Protein
DNA repair endonuclease XPF
Gene
mei-9
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Implicated in recombination events during meiosis, mostly in meiotic exchange. May directly resolve Holliday junctions within recombination intermediates leading to DNA exchange. Also required for the repair of mismatches within meiotic heteroduplex DNA and for nucleotide excision repair.
GO - Molecular functioni
- damaged DNA binding Source: GO_Central
- protein heterodimerization activity Source: FlyBase
- single-stranded DNA binding Source: GO_Central
- single-stranded DNA endodeoxyribonuclease activity Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: FlyBase
- DNA recombination Source: FlyBase
- double-strand break repair Source: FlyBase
- double-strand break repair via homologous recombination Source: GO_Central
- female meiosis chromosome segregation Source: FlyBase
- female meiotic nuclear division Source: FlyBase
- meiotic chromosome segregation Source: FlyBase
- mismatch repair Source: FlyBase
- nucleotide-excision repair Source: FlyBase
- nucleotide-excision repair, DNA incision, 5'-to lesion Source: GO_Central
- nucleotide-excision repair involved in interstrand cross-link repair Source: GO_Central
- oocyte fate determination Source: FlyBase
- reciprocal meiotic recombination Source: FlyBase
- resolution of meiotic recombination intermediates Source: FlyBase
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair, Meiosis |
Enzyme and pathway databases
Reactomei | R-DME-5696395, Formation of Incision Complex in GG-NER R-DME-5696400, Dual Incision in GG-NER R-DME-6782135, Dual incision in TC-NER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mei-9 ORF Names:CG3697 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002707, mei-9 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleotide-excision repair factor 1 complex Source: GO_Central
Other locations
- protein-containing complex Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198855 | 1 – 961 | DNA repair endonuclease XPFAdd BLAST | 961 |
Proteomic databases
PaxDbi | Q24087 |
Expressioni
Gene expression databases
Bgeei | FBgn0002707, Expressed in cleaving embryo and 29 other tissues |
ExpressionAtlasi | Q24087, baseline and differential |
Genevisiblei | Q24087, DM |
Interactioni
Subunit structurei
Heterodimer (By similarity).
Interacts with hdm.
By similarity1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 57890, 5 interactors |
IntActi | Q24087, 2 interactors |
STRINGi | 7227.FBpp0070624 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 697 – 777 | ERCC4Add BLAST | 81 |
Sequence similaritiesi
Belongs to the XPF family.Curated
Phylogenomic databases
eggNOGi | KOG0442, Eukaryota |
HOGENOMi | CLU_002265_1_0_1 |
InParanoidi | Q24087 |
OMAi | FHKILQA |
OrthoDBi | 324863at2759 |
PhylomeDBi | Q24087 |
Family and domain databases
InterProi | View protein in InterPro IPR006166, ERCC4_domain IPR011335, Restrct_endonuc-II-like IPR010994, RuvA_2-like IPR006167, XPF |
Pfami | View protein in Pfam PF02732, ERCC4, 1 hit |
SMARTi | View protein in SMART SM00891, ERCC4, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR00596, rad1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q24087-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADSCAENAA KGTENERPKE VEASADTVPQ VEEGVEEYLK RKNMVLLDYE
60 70 80 90 100
KQMFLDLVEA DGLLVCAKGL SYDRVVISIL KAYSDSGNLV LVINSSDWEE
110 120 130 140 150
QYYKSKIEPK YVHEVASTAT ERERVYLEGG LQFISTRILV VDLLKQRIPI
160 170 180 190 200
ELISGIIVLR AHTIIESCQE AFALRLFRQK NKTGFVKAFS SSPEAFTIGY
210 220 230 240 250
SHVERTMRNL FVKHLYIWPR FHESVRTVLQ PWKIQSIEMH VPISQNITSI
260 270 280 290 300
QSHILEIMNF LVQEIKRINR TVDMEAVTVE NCVTKSFHKI LQAQLDCIWH
310 320 330 340 350
QLNSQTKLIV ADLKILRSLM ISTMYHDAVS AYAFMKRYRS TEYALSNSGW
360 370 380 390 400
TLLDAAEQIF KLSRQRVFNG QQEFEPEPCP KWQTLTDLLT KEIPGDMRRS
410 420 430 440 450
RRSEQQPKVL ILCQDARTCH QLKQYLTQGG PRFLLQQALQ HEVPVGKLSD
460 470 480 490 500
NYAKESQTRS APPKNVSSNK ELRREEVSGS QPPLAGMDEL AQLLSESETE
510 520 530 540 550
GQHFEESYML TMTQPVEVGP AAIDIKPDPD VSIFETIPEL EQFDVTAALA
560 570 580 590 600
SVPHQPYICL QTFKTEREGS MALEHMLEQL QPHYVVMYNM NVTAIRQLEV
610 620 630 640 650
FEARRRLPPA DRMKVYFLIH ARTVEEQAYL TSLRREKAAF EFIIDTKSKM
660 670 680 690 700
VIPKYQDGKT DEAFLLLKTY DDEPTDENAK SRQAGGQAPQ ATKETPKVIV
710 720 730 740 750
DMREFRSDLP CLIHKRGLEV LPLTITIGDY ILTPDICVER KSISDLIGSL
760 770 780 790 800
NSGRLYNQCV QMQRHYAKPI LLIEFDQNKP FHLQGKFMLS QQTSMANADI
810 820 830 840 850
VQKLQLLTLH FPKLRLIWSP SPYATAQLFE ELKLGKPEPD PQTAAALGSD
860 870 880 890 900
EPTAGEQLHF NSGIYDFLLR LPGVHTRNIH GLLRKGGSLR QLLLRSQKEL
910 920 930 940 950
EELLQSQESA KLLYDILHVA HLPEKDEVTG STALLAASKQ FGAGSHNRFR
960
MAAAASRRGR R
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketT2FFI3 | T2FFI3_DROME | FI21265p1 | mei-9 DhR1, Dmel\CG3697, DmXPF, MEI-9, Mei-9 | 961 | Annotation score: |
Sequence cautioni
The sequence AAC46917 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | V → G in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 286 | S → T in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 286 | S → T in AAN71074 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 318 | S → T in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 594 | A → P in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 798 | A → R in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 853 | T → M in AAC46917 (PubMed:8647398).Curated | 1 | |
Sequence conflicti | 853 | T → M in AAN71074 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27181 Genomic DNA Translation: AAC46917.1 Frameshift. AE014298 Genomic DNA Translation: AAF45938.1 BT001319 mRNA Translation: AAN71074.1 |
PIRi | S58936 |
RefSeqi | NP_001284859.1, NM_001297930.1 NP_525068.1, NM_080329.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0070656; FBpp0070624; FBgn0002707 FBtr0343574; FBpp0310174; FBgn0002707 |
GeneIDi | 31373 |
KEGGi | dme:Dmel_CG3697 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27181 Genomic DNA Translation: AAC46917.1 Frameshift. AE014298 Genomic DNA Translation: AAF45938.1 BT001319 mRNA Translation: AAN71074.1 |
PIRi | S58936 |
RefSeqi | NP_001284859.1, NM_001297930.1 NP_525068.1, NM_080329.3 |
3D structure databases
SMRi | Q24087 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 57890, 5 interactors |
IntActi | Q24087, 2 interactors |
STRINGi | 7227.FBpp0070624 |
Proteomic databases
PaxDbi | Q24087 |
Genome annotation databases
EnsemblMetazoai | FBtr0070656; FBpp0070624; FBgn0002707 FBtr0343574; FBpp0310174; FBgn0002707 |
GeneIDi | 31373 |
KEGGi | dme:Dmel_CG3697 |
Organism-specific databases
CTDi | 31373 |
FlyBasei | FBgn0002707, mei-9 |
Phylogenomic databases
eggNOGi | KOG0442, Eukaryota |
HOGENOMi | CLU_002265_1_0_1 |
InParanoidi | Q24087 |
OMAi | FHKILQA |
OrthoDBi | 324863at2759 |
PhylomeDBi | Q24087 |
Enzyme and pathway databases
Reactomei | R-DME-5696395, Formation of Incision Complex in GG-NER R-DME-5696400, Dual Incision in GG-NER R-DME-6782135, Dual incision in TC-NER |
Miscellaneous databases
BioGRID-ORCSi | 31373, 0 hits in 1 CRISPR screen |
GenomeRNAii | 31373 |
PROi | PR:Q24087 |
Gene expression databases
Bgeei | FBgn0002707, Expressed in cleaving embryo and 29 other tissues |
ExpressionAtlasi | Q24087, baseline and differential |
Genevisiblei | Q24087, DM |
Family and domain databases
InterProi | View protein in InterPro IPR006166, ERCC4_domain IPR011335, Restrct_endonuc-II-like IPR010994, RuvA_2-like IPR006167, XPF |
Pfami | View protein in Pfam PF02732, ERCC4, 1 hit |
SMARTi | View protein in SMART SM00891, ERCC4, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR00596, rad1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XPF_DROME | |
Accessioni | Q24087Primary (citable) accession number: Q24087 Secondary accession number(s): Q8IHB9, Q9W4L3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families