Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

MutS protein homolog him-14

Gene

him-14

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required during the pachytene stage of meiotic prophase for the formation of crossovers between homologous chromosomes. Not needed for pairing or synapsis. May promote crossing over by interfering with Holliday junction branch migration. Has no apparent role in DNA mismatch repair.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi588 – 595ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA recombination, Meiosis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MutS protein homolog him-14
Alternative name(s):
High incidence of males protein 14
MutS protein homolog 4
Gene namesi
Name:him-14
Synonyms:msh-4
ORF Names:ZK1127.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiZK1127.11 ; CE24714 ; WBGene00001872 ; him-14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms show maternal effect lethality producing 95% inviable progeny from hermaphrodites. A high proportion of those that survive are male. Mutants display severely reduced crossing over, resulting in lack of chiasmata between homologous chromosomes and consequent missegregation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116G → E in me15; defective meiotic chromosomal segregation. 1 Publication1
Mutagenesisi238T → I in it13; defective meiotic chromosomal segregation. 1 Publication1
Mutagenesisi406D → N in it44; induces temperature sensitivity; defective meiotic chromosomal segregation. 1 Publication1
Mutagenesisi538S → L in it23; defective meiotic chromosomal segregation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001151991 – 842MutS protein homolog him-14Add BLAST842

Proteomic databases

PaxDbiQ23405
PRIDEiQ23405

Expressioni

Developmental stagei

Expressed maternally.1 Publication

Gene expression databases

BgeeiWBGene00001872 Expressed in 4 organ(s), highest expression level in germ line (C elegans)

Interactioni

Subunit structurei

Heterooligomer of him-14 and msh-5.By similarity

Protein-protein interaction databases

STRINGi6239.ZK1127.11

Structurei

3D structure databases

ProteinModelPortaliQ23405
SMRiQ23405
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0220 Eukaryota
COG0249 LUCA
GeneTreeiENSGT00550000074897
HOGENOMiHOG000015880
InParanoidiQ23405
KOiK08740
OMAiMREMAFI
OrthoDBiEOG091G026W
PhylomeDBiQ23405

Family and domain databases

Gene3Di3.30.420.110, 1 hit
InterProiView protein in InterPro
IPR011184 DNA_mismatch_repair_Msh2
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF005813 MSH2, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q23405-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYSNKSFQRR QRQQVAESRS EEKFSRSLVR LNAQSLLDSS GNNTTTKNVN
60 70 80 90 100
SDVVIVVMEG RGSCEGHIGI ALHDTCFPEI HLCEFVDSRE YTTLKTMINV
110 120 130 140 150
HEAFDIVIQN GNEERGSTKL LGEALMTAFP EASLQSISSK YFNSERGERQ
160 170 180 190 200
LQSLMNAEVS TVSEGCLRRT LALGALAVLL KYIHETRCVF FRVKSLRIKE
210 220 230 240 250
MGVNDTCMID FVSWESLEIV DADDASKARK FQMKQKRTLM SVLNHTVTTN
260 270 280 290 300
GYRLLRSSVL QPSTDVYLIQ SRQEAIEELI GKPQLKDKLR RTLSRAHELD
310 320 330 340 350
RVIAMCIQTS TSWTVRESEA KINQIIKLMH TLKVIQGIRT LLHSAKMKSN
360 370 380 390 400
ILIEKTEFLK DPRFDQIMNI LVEKVDDSLL DGKKNSLHLQ NTKCYAIRHF
410 420 430 440 450
VAVQLDLARQ TYEEIIRNVE ETGAREIAEY FHGNSSVRLS FSQSRGFHYT
460 470 480 490 500
FVTRQAESVT IPRYFLDVFR NRTTVTFNSR KVIAYNDRLE QVVAEMFLAS
510 520 530 540 550
DVIVCDMIEE MQPMIPVLYY AMDALSSIDF LCGLATYSDL RDTCKPTFGP
560 570 580 590 600
SFSISQGRHP ILDWDDSEKT ITNDTCLTRD RRFGIITGPN MAGKSTYLKQ
610 620 630 640 650
TAQLAIMAQI GCFIPANYAS LPIFNRIFSR MGHNDELIRN KSAFASEMSD
660 670 680 690 700
AAAIVQYADK NSLVVLDELA RSTSTEEGIA ITYAICEKVL KLQSYTFLAT
710 720 730 740 750
HFLDIAALAN YSNAIDNYHF LPQTDENSTK KHKLLRGQYR GPLYGFELVE
760 770 780 790 800
LSTIPDEVIE HAQSLATELR ANVEDTERDY DSERRRIKVY MNHRFRECAE
810 820 830 840
YFMDTHGEKW KEEKEAIDKM KALRKYLVDE LAKIDSQEQM CQ
Length:842
Mass (Da):96,331
Last modified:May 1, 2000 - v2
Checksum:i64D71CEF45F8157B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178755 mRNA Translation: AAD52669.1
FO081697 Genomic DNA Translation: CCD73725.1
PIRiE88197
RefSeqiNP_495451.1, NM_063050.5
UniGeneiCel.22695

Genome annotation databases

EnsemblMetazoaiZK1127.11; ZK1127.11; WBGene00001872
GeneIDi174157
KEGGicel:CELE_ZK1127.11
UCSCiZK1127.11 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178755 mRNA Translation: AAD52669.1
FO081697 Genomic DNA Translation: CCD73725.1
PIRiE88197
RefSeqiNP_495451.1, NM_063050.5
UniGeneiCel.22695

3D structure databases

ProteinModelPortaliQ23405
SMRiQ23405
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZK1127.11

Proteomic databases

PaxDbiQ23405
PRIDEiQ23405

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK1127.11; ZK1127.11; WBGene00001872
GeneIDi174157
KEGGicel:CELE_ZK1127.11
UCSCiZK1127.11 c. elegans

Organism-specific databases

CTDi174157
WormBaseiZK1127.11 ; CE24714 ; WBGene00001872 ; him-14

Phylogenomic databases

eggNOGiKOG0220 Eukaryota
COG0249 LUCA
GeneTreeiENSGT00550000074897
HOGENOMiHOG000015880
InParanoidiQ23405
KOiK08740
OMAiMREMAFI
OrthoDBiEOG091G026W
PhylomeDBiQ23405

Miscellaneous databases

PROiPR:Q23405

Gene expression databases

BgeeiWBGene00001872 Expressed in 4 organ(s), highest expression level in germ line (C elegans)

Family and domain databases

Gene3Di3.30.420.110, 1 hit
InterProiView protein in InterPro
IPR011184 DNA_mismatch_repair_Msh2
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF005813 MSH2, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHIM14_CAEEL
AccessioniPrimary (citable) accession number: Q23405
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: September 12, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again