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Protein

Ribokinase

Gene

TTHERM_00312950

Organism
Tetrahymena thermophila (strain SB210)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + D-ribose = ADP + D-ribose 5-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation

Activity regulationi

Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation

Pathwayi: D-ribose degradation

This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ribokinase (TTHERM_00312950)
This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateUniRule annotation1
Binding sitei191ATPUniRule annotation1
Metal bindingi265PotassiumUniRule annotation1
Metal bindingi267Potassium; via carbonyl oxygenUniRule annotation1
Active sitei271Proton acceptorUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Binding sitei307ATPUniRule annotation1
Metal bindingi313Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi316Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi318Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi322PotassiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi230 – 235ATPUniRule annotation6
Nucleotide bindingi270 – 271ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, Transferase
Biological processCarbohydrate metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00916;UER00889

Names & Taxonomyi

Protein namesi
Recommended name:
RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)
Short name:
RKUniRule annotation
Gene namesi
ORF Names:TTHERM_00312950Imported
OrganismiTetrahymena thermophila (strain SB210)Imported
Taxonomic identifieri312017 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena
Proteomesi
  • UP000009168 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi5911.EAR85813

Structurei

3D structure databases

ProteinModelPortaliQ22KI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 325PfkBInterPro annotationAdd BLAST319

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Substrate bindingUniRule annotation3
Regioni43 – 47Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ22KI6
KOiK00852
OMAiVNQHEVA

Family and domain databases

CDDicd01174 ribokinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01987 Ribokinase, 1 hit
InterProiView protein in InterPro
IPR011877 D_ribokin
IPR011611 PfkB_dom
IPR002139 Ribo/fructo_kinase
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF00294 PfkB, 1 hit
PRINTSiPR00990 RIBOKINASE
SUPFAMiSSF53613 SSF53613, 1 hit

Sequencei

Sequence statusi: Complete.

Q22KI6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQNTYSICV VGSGSFDLFL LVNHFPVEGE TLQANDSFIK NGGKGANQAV
60 70 80 90 100
AAAKLGAKTI FAGQFGQDKY GDGLVEEMSQ SGVDMSKVRR LANVQTGQAI
110 120 130 140 150
ITLNKKGENS IIIVGGANTH YDHLNVLPQE FKDAIQSSRI ILLQKEIPLE
160 170 180 190 200
ISKLAAIYAK QHNKLVMLDC GGRDEDIPEE LLQNIDFISP NQSELKRVMN
210 220 230 240 250
INSSDHGEIT IDEIRQQLLS KYPNLTVVLK EGSKGSAVIT KTDYLHVDSA
260 270 280 290 300
SLIKPDILNT YKIVDTTGAG DCFTAAFCVE FDKLFPQNGV QYEDALEKYK
310 320 330
KCLLFANCSA FVCITVMGAM PSMPKLESVQ QLLSKVQ
Length:337
Mass (Da):36,861
Last modified:May 29, 2007 - v2
Checksum:iDAD052BA8A22BE1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG662498 Genomic DNA Translation: EAR85813.2
RefSeqiXP_001033476.2, XM_001033476.3

Genome annotation databases

EnsemblProtistsiEAR85813; EAR85813; TTHERM_00312950
GeneIDi7825277
KEGGitet:TTHERM_00312950

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG662498 Genomic DNA Translation: EAR85813.2
RefSeqiXP_001033476.2, XM_001033476.3

3D structure databases

ProteinModelPortaliQ22KI6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5911.EAR85813

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAR85813; EAR85813; TTHERM_00312950
GeneIDi7825277
KEGGitet:TTHERM_00312950

Phylogenomic databases

InParanoidiQ22KI6
KOiK00852
OMAiVNQHEVA

Enzyme and pathway databases

UniPathwayi
UPA00916;UER00889

Family and domain databases

CDDicd01174 ribokinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01987 Ribokinase, 1 hit
InterProiView protein in InterPro
IPR011877 D_ribokin
IPR011611 PfkB_dom
IPR002139 Ribo/fructo_kinase
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF00294 PfkB, 1 hit
PRINTSiPR00990 RIBOKINASE
SUPFAMiSSF53613 SSF53613, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ22KI6_TETTS
AccessioniPrimary (citable) accession number: Q22KI6
Entry historyiIntegrated into UniProtKB/TrEMBL: April 18, 2006
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 57 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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