bioB

Functionⁱ

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityⁱ

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactorⁱ

Protein has several cofactor binding sites:

[4Fe-4S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
[2Fe-2S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694

Pathwayⁱ: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

ATP-dependent dethiobiotin synthetase BioD (bioD)
Biotin synthase (bioB)

This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	57	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	61	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	64	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	101	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	132	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	192	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	264	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
biotin synthase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

biotin biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Biotin biosynthesis
Ligand	2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	SDEG203122:G1G67-3308-MONOMER.
UniPathwayⁱ	UPA00078; UER00162.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesⁱ	Name:bioBUniRule annotation Ordered Locus Names:Sde_3139
Organismⁱ	Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Taxonomic identifierⁱ	203122 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Saccharophagus › Saccharophagus degradans
Proteomesⁱ	UP000001947 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000381592	1 – 350	Biotin synthaseAdd BLAST		350

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	203122.Sde_3139.

STRINGⁱ

203122.Sde_3139.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q21FY3.
SMRⁱ	Q21FY3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239957.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDF00272. biotin_synthase. 1 hit. SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q21FY3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSNPSLVRSN WTRKEVEALF ALPFNDLLFE AQTIHRQFFN ANEVQVSTLC 
        60         70         80         90        100
SIKTGACPED CAYCPQSARY DTGLEREKLM AVEKVLEEAK AAKDSGATRF 
       110        120        130        140        150
CMGAAWRSPK KKDMPYVTAM VKGVKEMGLE ACMTLGMIDK DQATELAEAG 
       160        170        180        190        200
LDYYNHNLDT SPEYYGDIIT TRTYQDRLDT LDNVRHAGMK VCCGGIMGMG 
       210        220        230        240        250
EDESDRVGLL LQLANMPQHP ESVPINMLVA VEGTPLEQQE ALDPFDFIRT 
       260        270        280        290        300
IAVARILMPQ SHVRLSAGRE DMNDQTQALA FHAGANSIFY GEKLLTTANP 
       310        320        330        340        350
ESNKDMQLFK RLGINPEKRE EHKTEQEQVE QINQQVAEAA LDKVFYNAAS

Length:350

Mass (Da):39,090

Last modified:April 18, 2006 - v1

Checksum:ⁱ4E24A73556B78742

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000282 Genomic DNA. Translation: ABD82396.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011469612.1. NC_007912.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABD82396; ABD82396; Sde_3139.
KEGGⁱ	sde:Sde_3139.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q21FY3	O59778 A0A1C3EPT1 A0A060NJ93 A0A1J5SCW7 UPI0009DEAA5B UPI00034B0D58 UPI000A03DE33 UPI0005F7A8EF A0A1I7NS66 UPI000255A30A +741	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) Enterovibrio pacificus Comamonadaceae bacterium A1 mine drainage metagenome Paraburkholderia kururiensis Sphingomonas hankookensis Hyphomicrobium sp. 99 Hyphomicrobium facile Marinobacterium stanieri Marinobacterium georgiense And more	363	UniRef50_O59778	Cluster: Biotin synthase	752

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000282 Genomic DNA. Translation: ABD82396.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011469612.1. NC_007912.1.

3D structure databases

ProteinModelPortalⁱ	Q21FY3.
SMRⁱ	Q21FY3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	203122.Sde_3139.

STRINGⁱ

203122.Sde_3139.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABD82396; ABD82396; Sde_3139.
KEGGⁱ	sde:Sde_3139.

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239957.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Enzyme and pathway databases

UniPathwayⁱ	UPA00078; UER00162.
BioCycⁱ	SDEG203122:G1G67-3308-MONOMER.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDF00272. biotin_synthase. 1 hit. SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BIOB_SACD2
Accessionⁱ	Q21FY3Primary (citable) accession number: Q21FY3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
	Last sequence update:	April 18, 2006
	Last modified:	February 28, 2018
	This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q21FY3 (BIOB_SACD2)

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Biotin synthase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: biotin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: biotin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ