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UniProtKB - Q21929 (DCXR_CAEEL)
Protein
L-xylulose reductase
Gene
dhs-21
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the NADPH-dependent reduction of L-xylulose, D-xylulose, L-+ erythrulose, D-erythrose, D-threose, L-ribulose, 1,4-dibromo-2,3-butanedione and 2,3-heptanedione (PubMed:21477590).
Also active against isatin, 9,10-phenanthrenequinone, menadione, 2,3-hexaenadione and 3,4-hexahenadione (PubMed:21300042).
No activity observed when tested using NADH rather than NADPH (PubMed:21300042).
2 PublicationsCatalytic activityi
- EC:1.1.1.101 Publication
Activity regulationi
Strongly inhibited by 10% dimethyl sulfoxide.1 Publication
Kineticsi
The KM value for isatin is approximate as substrate saturation was not reached.1 Publication
- KM=2.65 mM for isatin (at 25 degrees Celsius)1 Publication
- KM=0.66 mM for L-xylulose (at 25 degrees Celsius)1 Publication
- KM=0.17 mM for L-+ erythrulose (at 25 degrees Celsius)1 Publication
- KM=0.27 mM for D-erythrose (at 25 degrees Celsius)1 Publication
- KM=0.68 mM for D-threose (at 25 degrees Celsius)1 Publication
- KM=2.58 mM for D-xylulose (at 25 degrees Celsius)1 Publication
- KM=0.18 mM for L-ribulose (at 25 degrees Celsius)1 Publication
- KM=8.54 mM for 1,4-dibromo-2,3-butanedione (at 25 degrees Celsius)1 Publication
- KM=0.02 mM for 2,3-heptanedione (at 25 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 143 | SubstrateBy similarity | 1 | |
Active sitei | 156 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 160 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 42 | NADPBy similarityAdd BLAST | 30 |
GO - Molecular functioni
- carbonyl reductase (NADPH) activity Source: UniProtKB
- catalytic activity Source: UniProtKB
- L-xylulose reductase (NADP+) activity Source: WormBase
GO - Biological processi
- D-xylose metabolic process Source: UniProtKB-KW
- glucose metabolic process Source: UniProtKB
- xylulose catabolic process Source: WormBase
- xylulose metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Carbohydrate metabolism, Xylose metabolism |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.10, 1045 |
Reactomei | R-CEL-5661270, Formation of xylulose-5-phosphate |
SABIO-RKi | Q21929 |
Names & Taxonomyi
Protein namesi | Recommended name: L-xylulose reductaseBy similarity (EC:1.1.1.101 Publication)Short name: XR Alternative name(s): Dicarbonyl/L-xylulose reductase1 Publication Short name: DCXR1 Publication Short-chain dehydrogenase 21Imported |
Gene namesi | Name:dhs-21Imported ORF Names:R11D1.11Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | R11D1.11 ; CE39954 ; WBGene00000984 ; dhs-21 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Note: Detected on the cell membrane of spermatids.1 Publication
Plasma Membrane
- plasma membrane Source: WormBase
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Reduced life span and impaired egg-laying function resulting in the retention of eggs. Exogenous addition of serotonin, which triggers vulval muscle contraction, or imipranine, a serotonin uptake inhibitor, rescues the ability to lay eggs, showing that the morphology of the vulva muscles and HSN neurons is normal.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054549 | 1 – 251 | L-xylulose reductaseAdd BLAST | 251 |
Proteomic databases
EPDi | Q21929 |
PaxDbi | Q21929 |
PeptideAtlasi | Q21929 |
Expressioni
Tissue specificityi
Expressed in intestine, gonad and spermatids (at protein level). Expressed in intestine, uterine seam, gonadal sheath cells, spermathecal-uterus valve and spermatids.1 Publication
Developmental stagei
Detected at the two-fold embryo stage. Expressed in the intestine during L1 to L3, followed by expression in uterine seam, gonadal sheath cells and spermathecal-uterus valve at L4.1 Publication
Gene expression databases
Bgeei | WBGene00000984, Expressed in material anatomical entity and 4 other tissues |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGRIDi | 44761, 39 interactors |
STRINGi | 6239.R11D1.11 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG1207, Eukaryota |
GeneTreei | ENSGT00940000154873 |
HOGENOMi | CLU_010194_1_1_1 |
InParanoidi | Q21929 |
OMAi | VYLCCKA |
OrthoDBi | 1051625at2759 |
PhylomeDBi | Q21929 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q21929-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPANYDFTDK RILVTGASQG IGKEICLSLA KAGAQVIAFA RNEANLLSLV
60 70 80 90 100
KETTSLRYTI IPIVGDVSAN EEVLFKLIVP HFPIHGLVNN AGIATNHAIG
110 120 130 140 150
QITQQSIDRT FAVNVRGPIL IAQLVARNFV DRQIKGSIVN ISSQAAIRPL
160 170 180 190 200
DNHTVYCASK AALDMVTRCL ANELGSQNIR VNSVNPTVVM TDMGRDNWSD
210 220 230 240 250
PDKKKKMLDR MPIKRFAEVD EVVNAVLFLL SDNASMTTGS TLPVDGGFSN
N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75547, Z75527 Genomic DNA Translation: CAA99897.2 |
PIRi | T24180 |
RefSeqi | NP_506182.2, NM_073781.6 |
Genome annotation databases
EnsemblMetazoai | R11D1.11.1; R11D1.11.1; WBGene00000984 |
GeneIDi | 179741 |
KEGGi | cel:CELE_R11D1.11 |
UCSCi | R11D1.11, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75547, Z75527 Genomic DNA Translation: CAA99897.2 |
PIRi | T24180 |
RefSeqi | NP_506182.2, NM_073781.6 |
3D structure databases
SMRi | Q21929 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 44761, 39 interactors |
STRINGi | 6239.R11D1.11 |
Proteomic databases
EPDi | Q21929 |
PaxDbi | Q21929 |
PeptideAtlasi | Q21929 |
Genome annotation databases
EnsemblMetazoai | R11D1.11.1; R11D1.11.1; WBGene00000984 |
GeneIDi | 179741 |
KEGGi | cel:CELE_R11D1.11 |
UCSCi | R11D1.11, c. elegans |
Organism-specific databases
CTDi | 179741 |
WormBasei | R11D1.11 ; CE39954 ; WBGene00000984 ; dhs-21 |
Phylogenomic databases
eggNOGi | KOG1207, Eukaryota |
GeneTreei | ENSGT00940000154873 |
HOGENOMi | CLU_010194_1_1_1 |
InParanoidi | Q21929 |
OMAi | VYLCCKA |
OrthoDBi | 1051625at2759 |
PhylomeDBi | Q21929 |
Enzyme and pathway databases
BRENDAi | 1.1.1.10, 1045 |
Reactomei | R-CEL-5661270, Formation of xylulose-5-phosphate |
SABIO-RKi | Q21929 |
Miscellaneous databases
PROi | PR:Q21929 |
Gene expression databases
Bgeei | WBGene00000984, Expressed in material anatomical entity and 4 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCXR_CAEEL | |
Accessioni | Q21929Primary (citable) accession number: Q21929 Secondary accession number(s): Q27GP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | September 5, 2006 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families