UniProtKB - Q21443 (LMN1_CAEEL)
Protein
Lamin-1
Gene
lmn-1
Organism
Caenorhabditis elegans
Status
Functioni
Major component of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:11071918, PubMed:25057012). Provides a framework for the nuclear envelope and probably also interacts with chromatin (PubMed:11071918, PubMed:25057012). Essential to maintain the shape and integrity of the nucleus, and for DNA replication (PubMed:11071918). Involved in spatial organization of nuclear pore complexes (PubMed:11071918). It is not a target for ced-3 during apoptosis, suggesting that lamin cleavage is not essential for apoptosis in C.elegans (PubMed:12064941).3 Publications
Miscellaneous
It is the only lamin protein in C.elegans.
GO - Molecular functioni
- histone binding Source: WormBase
- identical protein binding Source: IntAct
- structural molecule activity Source: WormBase
GO - Biological processi
- determination of adult lifespan Source: UniProtKB
- embryo development ending in birth or egg hatching Source: UniProtKB
- germ cell development Source: WormBase
- nuclear migration along microtubule Source: WormBase
- nuclear pore localization Source: WormBase
- nucleus organization Source: WormBase
- positive regulation of cell population proliferation Source: WormBase
- protein localization Source: WormBase
- regulation of chromosome segregation Source: WormBase
- regulation of mitotic cell cycle Source: WormBase
Enzyme and pathway databases
Reactomei | R-CEL-4419969, Depolymerisation of the Nuclear Lamina |
Names & Taxonomyi
Protein namesi | Recommended name: Lamin-1Alternative name(s): Ce-lamin CeLam-1 |
Gene namesi | ORF Names:DY3.2Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | DY3.2 ; CE15746 ; WBGene00003052 ; lmn-1 |
Subcellular locationi
Nucleus
- Nucleus envelope 1 Publication
- Nucleus inner membrane 5 Publications; Lipid-anchor 3 Publications; Nucleoplasmic side 3 Publications
Note: Remains in the nuclear envelope until late anaphase in early embryos. Depends on mel-28 for nuclear envelope localization after mitosis.2 Publications
Cytoskeleton
- lamin filament Source: WormBase
Nucleus
- lamin filament Source: WormBase
- nuclear envelope Source: WormBase
- nuclear inner membrane Source: UniProtKB-SubCell
- nuclear periphery Source: WormBase
Keywords - Cellular componenti
Intermediate filament, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063826 | 2 – 563 | Lamin-1Add BLAST | 562 | |
PropeptideiPRO_0000403473 | 564 – 566 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 563 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 563 | S-farnesyl cysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | Q21443 |
PaxDbi | Q21443 |
PeptideAtlasi | Q21443 |
Expressioni
Tissue specificityi
Ubiquitous. Expressed in all cells, except in cells undergoing spermatogenesis.1 Publication
Developmental stagei
Expressed throughout the development and in adults.1 Publication
Gene expression databases
Bgeei | WBGene00003052, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ21443
With | #Exp. | IntAct |
---|---|---|
lem-2 [Q9XTB5] | 3 | EBI-314110,EBI-2535391 |
itself | 3 | EBI-314110,EBI-314110 |
GO - Molecular functioni
- histone binding Source: WormBase
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 38120, 65 interactors |
DIPi | DIP-29640N |
IntActi | Q21443, 43 interactors |
STRINGi | 6239.DY3.2.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 387 | IF rodPROSITE-ProRule annotationAdd BLAST | 343 | |
Domaini | 435 – 550 | LTDPROSITE-ProRule annotationAdd BLAST | 116 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 47 | HeadAdd BLAST | 35 | |
Regioni | 48 – 82 | Coil 1AAdd BLAST | 35 | |
Regioni | 83 – 94 | Linker 1Add BLAST | 12 | |
Regioni | 95 – 228 | Coil 1BAdd BLAST | 134 | |
Regioni | 229 – 256 | Linker 2Add BLAST | 28 | |
Regioni | 257 – 385 | Coil 2Add BLAST | 129 | |
Regioni | 386 – 566 | TailAdd BLAST | 181 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000168319 |
HOGENOMi | CLU_012560_9_2_1 |
InParanoidi | Q21443 |
OMAi | CGQPAEK |
OrthoDBi | 701388at2759 |
PhylomeDBi | Q21443 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q21443-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSRKGTRSS RIVTLERSAN SSLSNNGGGD DSFGSTLLET SRLQEKDHLT
60 70 80 90 100
SLNSRLATYI DKVRQLEQEN NRLQVQIRDI EVVEKKEKSN LADRFEAEKA
110 120 130 140 150
RLRRALDSAQ DELAKYRIEY DAAKVEVKKL KPQVEKLERE LAGAEEQALH
160 170 180 190 200
AQSIADQSQA KQKTLQARND KLVVENDDLK KQNITLRDTV EGLKKAVEDE
210 220 230 240 250
TLLRTAANNK IKALEEDLAF ALQQHKGELE EVRHKRQVDM TTYAKQINDE
260 270 280 290 300
YQSKLQDQIE EMRAQFKNNL HQNKTAFEDA YKNKLNAARE RQEEAVSEAI
310 320 330 340 350
HLRARVRDLE TSSSGNASLI ERLRSELDTL KRSFQEKLDD KDARIAELNQ
360 370 380 390 400
EIERMMSEFH DLLDVKIQLD AELKTYQALL EGEEERLNLT QEAPQNTSVH
410 420 430 440 450
HVSFSSGGAS AQRGVKRRRV VDVNGEDQDI DYLNRRSKLN KETVGPVGID
460 470 480 490 500
EVDEEGKWVR VANNSEEEQS IGGYKLVVKA GNKEASFQFS SRMKLAPHAS
510 520 530 540 550
ATVWSADAGA VHHPPEVYVM KKQQWPIGDN PSARLEDSEG DTVSSITVEF
560
SESSDPSDPA DRCSIM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 499 | A → R in CAA52188 (PubMed:7925480).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74027 Genomic DNA Translation: CAA52188.1 BX284601 Genomic DNA Translation: CAB09411.1 |
PIRi | S42257 T20390 |
RefSeqi | NP_492371.1, NM_059970.4 |
Genome annotation databases
EnsemblMetazoai | DY3.2.1; DY3.2.1; WBGene00003052 DY3.2.2; DY3.2.2; WBGene00003052 |
GeneIDi | 172687 |
KEGGi | cel:CELE_DY3.2 |
UCSCi | DY3.2.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74027 Genomic DNA Translation: CAA52188.1 BX284601 Genomic DNA Translation: CAB09411.1 |
PIRi | S42257 T20390 |
RefSeqi | NP_492371.1, NM_059970.4 |
3D structure databases
SMRi | Q21443 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 38120, 65 interactors |
DIPi | DIP-29640N |
IntActi | Q21443, 43 interactors |
STRINGi | 6239.DY3.2.2 |
Proteomic databases
EPDi | Q21443 |
PaxDbi | Q21443 |
PeptideAtlasi | Q21443 |
Genome annotation databases
EnsemblMetazoai | DY3.2.1; DY3.2.1; WBGene00003052 DY3.2.2; DY3.2.2; WBGene00003052 |
GeneIDi | 172687 |
KEGGi | cel:CELE_DY3.2 |
UCSCi | DY3.2.1, c. elegans |
Organism-specific databases
CTDi | 172687 |
WormBasei | DY3.2 ; CE15746 ; WBGene00003052 ; lmn-1 |
Phylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000168319 |
HOGENOMi | CLU_012560_9_2_1 |
InParanoidi | Q21443 |
OMAi | CGQPAEK |
OrthoDBi | 701388at2759 |
PhylomeDBi | Q21443 |
Enzyme and pathway databases
Reactomei | R-CEL-4419969, Depolymerisation of the Nuclear Lamina |
Miscellaneous databases
PROi | PR:Q21443 |
Gene expression databases
Bgeei | WBGene00003052, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LMN1_CAEEL | |
Accessioni | Q21443Primary (citable) accession number: Q21443 Secondary accession number(s): O62127 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | April 26, 2004 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families