UniProtKB - Q21338 (SPT5H_CAEEL)
Transcription elongation factor SPT5
spt-5
Functioni
May function as a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. DSIF may enhance transcriptional pausing at sites proximal to the promoter, which may in turn facilitate the assembly of an elongation competent RNA polymerase II complex.
1 PublicationGO - Molecular functioni
- mRNA binding Source: GO_Central
GO - Biological processi
- regulation of DNA-templated transcription, elongation Source: InterPro
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription elongation from RNA polymerase II promoter Source: GO_Central
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-CEL-112382, Formation of RNA Pol II elongation complex R-CEL-113418, Formation of the Early Elongation Complex R-CEL-674695, RNA Polymerase II Pre-transcription Events R-CEL-6796648, TP53 Regulates Transcription of DNA Repair Genes R-CEL-72086, mRNA Capping R-CEL-75955, RNA Polymerase II Transcription Elongation R-CEL-77075, RNA Pol II CTD phosphorylation and interaction with CE |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor SPT5Alternative name(s): DRB sensitivity-inducing factor large subunit Short name: DSIF large subunit |
Gene namesi | Name:spt-5 ORF Names:K08E4.1 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | K08E4.1 ; CE06145 ; WBGene00005015 ; spt-5 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- DSIF complex Source: UniProtKB
Other locations
- euchromatin Source: WormBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208474 | 1 – 1208 | Transcription elongation factor SPT5Add BLAST | 1208 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 815 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 822 | Phosphothreonine; by CDK9By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q21338 |
PaxDbi | Q21338 |
PeptideAtlasi | Q21338 |
PRIDEi | Q21338 |
PTM databases
iPTMneti | Q21338 |
Expressioni
Gene expression databases
Bgeei | WBGene00005015, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Subunit structurei
Interacts with spt-4 to form DSIF. DSIF interacts with RNA polymerase II and with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of cdk-9 and cyclin-T (cit-1.1 or cit-1.2) (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 43237, 9 interactors |
STRINGi | 6239.K08E4.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 420 – 447 | KOW 1Add BLAST | 28 | |
Domaini | 473 – 506 | KOW 2Add BLAST | 34 | |
Domaini | 595 – 629 | KOW 3Add BLAST | 35 | |
Domaini | 730 – 763 | KOW 4Add BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 116 | DisorderedSequence analysisAdd BLAST | 116 | |
Regioni | 151 – 176 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 182 – 274 | Interaction with SPT4By similarityAdd BLAST | 93 | |
Regioni | 317 – 421 | Interaction with RNA polymerase IIBy similarityAdd BLAST | 105 | |
Regioni | 658 – 726 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 792 – 812 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 836 – 1004 | DisorderedSequence analysisAdd BLAST | 169 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 45 | Basic and acidic residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 46 – 60 | Acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 86 – 109 | Acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 151 – 171 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 658 – 706 | Polar residuesSequence analysisAdd BLAST | 49 | |
Compositional biasi | 865 – 881 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 900 – 914 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 922 – 951 | Polar residuesSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1999, Eukaryota |
GeneTreei | ENSGT00440000037640 |
HOGENOMi | CLU_003537_0_0_1 |
InParanoidi | Q21338 |
OMAi | FLAWDVE |
OrthoDBi | 828863at2759 |
PhylomeDBi | Q21338 |
Family and domain databases
CDDi | cd06081, KOW_Spt5_1, 1 hit cd06082, KOW_Spt5_2, 1 hit cd06083, KOW_Spt5_3, 1 hit cd06084, KOW_Spt5_4, 1 hit cd06085, KOW_Spt5_5, 1 hit cd06086, KOW_Spt5_6, 1 hit cd09888, NGN_Euk, 1 hit |
Gene3Di | 2.30.30.30, 3 hits 3.30.70.940, 1 hit |
InterProi | View protein in InterPro IPR005824, KOW IPR041973, KOW_Spt5_1 IPR041975, KOW_Spt5_2 IPR041976, KOW_Spt5_3 IPR041977, KOW_Spt5_4 IPR041978, KOW_Spt5_5 IPR041980, KOW_Spt5_6 IPR005100, NGN-domain IPR006645, NGN_dom IPR036735, NGN_dom_sf IPR039385, NGN_Euk IPR014722, Rib_L2_dom2 IPR039659, SPT5 IPR024945, Spt5_C_dom IPR022581, Spt5_N IPR017071, TF_Spt5_eukaryote IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR11125, PTHR11125, 1 hit |
Pfami | View protein in Pfam PF00467, KOW, 1 hit PF03439, Spt5-NGN, 1 hit PF11942, Spt5_N, 1 hit |
PIRSFi | PIRSF036945, Spt5, 1 hit |
SMARTi | View protein in SMART SM01104, CTD, 2 hits SM00739, KOW, 5 hits SM00738, NGN, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSDESDAAS NKEEDTTLSD DDGSDVEIKN SKKDRKRKLA SESEKSGSDD
60 70 80 90 100
DDDDDDDEDE NVTSKKSKKQ KRKNKAPSGR DFLAWDVEVD DEDEDDDNDY
110 120 130 140 150
DDEDDPSMNA MNEREEAERA MKEMELSQRN RDRYKFQNMT EDEVQKYFEN
160 170 180 190 200
KYKGDKNDSQ YDDEDSAMDD ISKNSHLPST KDPNLWIVKC RMGEEKLVAM
210 220 230 240 250
HLMRKCLAVE HTNEPFQIKS VVVKEGLKGM IYIEAFKQSH VMSAIEGFSA
260 270 280 290 300
LNQFTITMVP IKDMVDVLRV VKDIPQLKLG SYVRLKRTMY KDDLAVVDLV
310 320 330 340 350
DIAQNRVNLK LIPRVDYQKR RGAMRTDADK NYKLKRRPMP KLFDQDTIKE
360 370 380 390 400
VGGEIVTDGD FLVFEGNHFR RGFLYKYFPI NAIQADGVKP TLGELEKFQE
410 420 430 440 450
SSDDLKRELE TASLKDTENP FVPGDIVEVK AGELVNLRGK VMTVDGTKVV
460 470 480 490 500
MMPDQEDLKE AITLNAHELR KYFKEGDHAK VISGRYEGHT GLIVRVKDST
510 520 530 540 550
AIVLSDLGME ELKVRVRDLQ LCADVTTGVD SLGQFQYHDL VQLDHTGNVG
560 570 580 590 600
VIVRLEKEHL EVLNMHGVVN RIKPQAIIAK KDVRFAKVLD SQNNSIEAKD
610 620 630 640 650
LVKVIGGPNA KERETDEDPV GEVLYAFRGT VFVYSRKVTK NGGVLVCKPK
660 670 680 690 700
QLILQGAKKT TSTPMVSRMA SPNPMASPRH SSGGMTPRSP QDGMSSRGSS
710 720 730 740 750
GGQTPRQGGG GGRGGHFGTA NQQKVRRDLT LIGKNVRIIK GPMKGHFGIV
760 770 780 790 800
RDATEDTVRV ELHTQCRTIS VDRARVMVVG DTGITAGSGG GSSFYSSSKT
810 820 830 840 850
PMRDSGKTPM YGSKTPMYGA QTPMYGSMTP AYDGGRTPAY GEGGRTPAYG
860 870 880 890 900
SKTPAYGDLD EHSSSRTPAY GNDSSRTPAY GSADGARTPA YGSTEGGRTP
910 920 930 940 950
AYGSMDNSRT PAYDDSGRTP GYESMPSRTP NYDSSSKTPA YPESEHSART
960 970 980 990 1000
PAYNNDYDIP LSPAYEPDAP EAYDNAPART PAFVSRTPGY DTYENSSPTY
1010 1020 1030 1040 1050
EPDAATKVEE DIGDTSSPTY DSPPHSYVVP TPGAMLNPAT PGAYHVDTPG
1060 1070 1080 1090 1100
FAAPMTPGSG GAYDQYVAPS PFAGYDSNNY NNADGAIEQI PDHFLAQGVW
1110 1120 1130 1140 1150
IVQNLCVQIK DHEGRFNGRE AIIKDVTDGK VDVYMPDHKC NLEVDFDQLT
1160 1170 1180 1190 1200
PMKPQPGDDA RVIFGQDAGH SGQLVSMDGF EAVIRSQEDM SDMRVINIGL
CCKMHSET
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z68316, Z68752 Genomic DNA Translation: CAA92685.1 |
PIRi | T23467 |
RefSeqi | NP_502283.1, NM_069882.5 |
Genome annotation databases
EnsemblMetazoai | K08E4.1.1; K08E4.1.1; WBGene00005015 K08E4.1.2; K08E4.1.2; WBGene00005015 |
GeneIDi | 178143 |
KEGGi | cel:CELE_K08E4.1 |
UCSCi | K08E4.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z68316, Z68752 Genomic DNA Translation: CAA92685.1 |
PIRi | T23467 |
RefSeqi | NP_502283.1, NM_069882.5 |
3D structure databases
AlphaFoldDBi | Q21338 |
SMRi | Q21338 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 43237, 9 interactors |
STRINGi | 6239.K08E4.1 |
PTM databases
iPTMneti | Q21338 |
Proteomic databases
EPDi | Q21338 |
PaxDbi | Q21338 |
PeptideAtlasi | Q21338 |
PRIDEi | Q21338 |
Genome annotation databases
EnsemblMetazoai | K08E4.1.1; K08E4.1.1; WBGene00005015 K08E4.1.2; K08E4.1.2; WBGene00005015 |
GeneIDi | 178143 |
KEGGi | cel:CELE_K08E4.1 |
UCSCi | K08E4.1, c. elegans |
Organism-specific databases
CTDi | 178143 |
WormBasei | K08E4.1 ; CE06145 ; WBGene00005015 ; spt-5 |
Phylogenomic databases
eggNOGi | KOG1999, Eukaryota |
GeneTreei | ENSGT00440000037640 |
HOGENOMi | CLU_003537_0_0_1 |
InParanoidi | Q21338 |
OMAi | FLAWDVE |
OrthoDBi | 828863at2759 |
PhylomeDBi | Q21338 |
Enzyme and pathway databases
Reactomei | R-CEL-112382, Formation of RNA Pol II elongation complex R-CEL-113418, Formation of the Early Elongation Complex R-CEL-674695, RNA Polymerase II Pre-transcription Events R-CEL-6796648, TP53 Regulates Transcription of DNA Repair Genes R-CEL-72086, mRNA Capping R-CEL-75955, RNA Polymerase II Transcription Elongation R-CEL-77075, RNA Pol II CTD phosphorylation and interaction with CE |
Miscellaneous databases
PROi | PR:Q21338 |
Gene expression databases
Bgeei | WBGene00005015, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
CDDi | cd06081, KOW_Spt5_1, 1 hit cd06082, KOW_Spt5_2, 1 hit cd06083, KOW_Spt5_3, 1 hit cd06084, KOW_Spt5_4, 1 hit cd06085, KOW_Spt5_5, 1 hit cd06086, KOW_Spt5_6, 1 hit cd09888, NGN_Euk, 1 hit |
Gene3Di | 2.30.30.30, 3 hits 3.30.70.940, 1 hit |
InterProi | View protein in InterPro IPR005824, KOW IPR041973, KOW_Spt5_1 IPR041975, KOW_Spt5_2 IPR041976, KOW_Spt5_3 IPR041977, KOW_Spt5_4 IPR041978, KOW_Spt5_5 IPR041980, KOW_Spt5_6 IPR005100, NGN-domain IPR006645, NGN_dom IPR036735, NGN_dom_sf IPR039385, NGN_Euk IPR014722, Rib_L2_dom2 IPR039659, SPT5 IPR024945, Spt5_C_dom IPR022581, Spt5_N IPR017071, TF_Spt5_eukaryote IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR11125, PTHR11125, 1 hit |
Pfami | View protein in Pfam PF00467, KOW, 1 hit PF03439, Spt5-NGN, 1 hit PF11942, Spt5_N, 1 hit |
PIRSFi | PIRSF036945, Spt5, 1 hit |
SMARTi | View protein in SMART SM01104, CTD, 2 hits SM00739, KOW, 5 hits SM00738, NGN, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT5H_CAEEL | |
Accessioni | Q21338Primary (citable) accession number: Q21338 Secondary accession number(s): Q22440 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families