UniProtKB - Q20616 (SKPO1_CAEEL)
Protein
Peroxidase skpo-1
Gene
skpo-1
Organism
Caenorhabditis elegans
Status
Functioni
Involved in hypodermal immune response against some types of bacterial infection. Probably utilizes H2O2 produced by the NADPH oxidase bli-3. May play a role in cuticule biosynthesis.1 Publication1 Publication
Catalytic activityi
- EC:1.11.1.7By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 222 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Sitei | 332 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Metal bindingi | 428 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: UniProtKB
GO - Biological processi
- defense response to Gram-positive bacterium Source: UniProtKB
- determination of adult lifespan Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- negative regulation of gene expression Source: UniProtKB
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Immunity, Innate immunity |
Ligand | Heme, Iron, Metal-binding |
Protein family/group databases
PeroxiBasei | 4139, CelPxd03 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxidase skpo-11 Publication (EC:1.11.1.7By similarity)Alternative name(s): ShKT and peroxidase domain-containing protein 1Imported |
Gene namesi | Name:skpo-1Imported ORF Names:F49E12.1Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | F49E12.1 ; CE03378 ; WBGene00009897 ; skpo-1 |
Pathology & Biotechi
Disruption phenotypei
Knockouts show increased susceptibility to infection with E.faecalis but not with P.aeruginosa, increased levels of H2O2 upon infection and a slightly shorter life span. Fertile animals show a bagging phenotype due to embryos being retained in the body and an increase of clec-60 mRNA levels upon infection. 50% of knockouts also have a dumpy phenotype. RNAi-mediated knockdown of the protein also results in higher susceptibility to infection by E.faecalis but not in a decreased life span or morphological changes.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000431239 | 20 – 655 | Peroxidase skpo-1Sequence analysisAdd BLAST | 636 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 22 ↔ 56 | PROSITE-ProRule annotation | ||
Disulfide bondi | 29 ↔ 49 | PROSITE-ProRule annotation | ||
Disulfide bondi | 38 ↔ 53 | PROSITE-ProRule annotation | ||
Disulfide bondi | 133 ↔ 150 | PROSITE-ProRule annotation | ||
Disulfide bondi | 520 ↔ 576 | PROSITE-ProRule annotation | ||
Disulfide bondi | 617 ↔ 642 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | Q20616 |
PaxDbi | Q20616 |
PeptideAtlasi | Q20616 |
Expressioni
Tissue specificityi
Exclusively expressed in hypodermis.1 Publication
Gene expression databases
Bgeei | WBGene00009897, Expressed in adult organism and 5 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 39669, 2 interactors |
STRINGi | 6239.F49E12.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 56 | ShKTPROSITE-ProRule annotationAdd BLAST | 35 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2408, Eukaryota |
HOGENOMi | CLU_006087_4_0_1 |
InParanoidi | Q20616 |
OMAi | CQTGDNM |
OrthoDBi | 276568at2759 |
PhylomeDBi | Q20616 |
Family and domain databases
Gene3Di | 1.10.640.10, 1 hit |
InterProi | View protein in InterPro IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR003582, ShKT_dom |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF01549, ShK, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00254, ShKT, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS50292, PEROXIDASE_3, 1 hit PS51670, SHKT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q20616-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSLLFSILL IYLIQLVRSE ECTDKHIHCF FWSQEGECEV NPRWMKKHCQ
60 70 80 90 100
KACGTCSLTS PTPLTRQQDV PTARTFDDQQ DRQFLPSRPS SIPEGCNSVM
110 120 130 140 150
TVEAETRRIF SSGQLTARFR QQMCAEEQVA PDCSINQCFH KKYRSMDGTC
160 170 180 190 200
NNLQNPVKGA AFTAFTRLMP AAYDDGFNTL VSASRRNRPN PREVSVFLLS
210 220 230 240 250
SERSLPGHVN SLLMLFGQFV SHDITSNAAQ NFCGCQNSGP MCASIFAPPS
260 270 280 290 300
DRSRRCIPFT RSFPICGTGQ FGRVREQLNM NTAAIDASLI YGSEAITARS
310 320 330 340 350
LRFAAMLRTS MIGGRMFPPN TNPGSLTAGD GRAILFVGLA ALHTSFLRLH
360 370 380 390 400
NNVAARLQNM NRHWNADRIF QESRKIVGGI VQVITYQEFV PELIGDASKT
410 420 430 440 450
ILGAYNGYNP NVEIGVLNEF AAGAYRLHGM IQETYPLVNS QFQEVNRYRF
460 470 480 490 500
IDGVNNINHV LNNIDAIYRG MMTVPVRSPQ RLTTSVTERL FGGSVDMAAV
510 520 530 540 550
NIQRGRDHGL RSYNDYRRFC NLRPITSFND WPEVPDENVR QRIGQLYRTP
560 570 580 590 600
DDLDFYVGGI LEQPAAGSLL GATFACVIGK QFERLRDGDR FYYENPGVFT
610 620 630 640 650
SPQLAELKRT TLSWVLCQTG DNMVRVGRRA FDIENGSRAV PCSSITGLNL
EAWRE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z66520 Genomic DNA Translation: CAA91388.1 |
PIRi | T22448 |
RefSeqi | NP_495768.1, NM_063367.5 |
Genome annotation databases
EnsemblMetazoai | F49E12.1.1; F49E12.1.1; WBGene00009897 |
GeneIDi | 174340 |
KEGGi | cel:CELE_F49E12.1 |
UCSCi | F49E12.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z66520 Genomic DNA Translation: CAA91388.1 |
PIRi | T22448 |
RefSeqi | NP_495768.1, NM_063367.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 39669, 2 interactors |
STRINGi | 6239.F49E12.1 |
Protein family/group databases
PeroxiBasei | 4139, CelPxd03 |
Proteomic databases
EPDi | Q20616 |
PaxDbi | Q20616 |
PeptideAtlasi | Q20616 |
Genome annotation databases
EnsemblMetazoai | F49E12.1.1; F49E12.1.1; WBGene00009897 |
GeneIDi | 174340 |
KEGGi | cel:CELE_F49E12.1 |
UCSCi | F49E12.1, c. elegans |
Organism-specific databases
CTDi | 174340 |
WormBasei | F49E12.1 ; CE03378 ; WBGene00009897 ; skpo-1 |
Phylogenomic databases
eggNOGi | KOG2408, Eukaryota |
HOGENOMi | CLU_006087_4_0_1 |
InParanoidi | Q20616 |
OMAi | CQTGDNM |
OrthoDBi | 276568at2759 |
PhylomeDBi | Q20616 |
Miscellaneous databases
PROi | PR:Q20616 |
Gene expression databases
Bgeei | WBGene00009897, Expressed in adult organism and 5 other tissues |
Family and domain databases
Gene3Di | 1.10.640.10, 1 hit |
InterProi | View protein in InterPro IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR003582, ShKT_dom |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF01549, ShK, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00254, ShKT, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS50292, PEROXIDASE_3, 1 hit PS51670, SHKT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SKPO1_CAEEL | |
Accessioni | Q20616Primary (citable) accession number: Q20616 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 26, 2014 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families